Identification and characterization of microRNAs expressed in chicken skeletal muscle

Andreote, Ana Paula Dini; Rosário, Millor Fernandes do; Ledur, Mônica Corrêa; Jorge, Érika Cristina; Sonstegard, Tad S.; Matukumalli, Lakshmi K.; Coutinho, Luiz Lehmann

doi:10.4238/2014.march.6.5

Cited by 9 publications

(7 citation statements)

References 39 publications

(42 reference statements)

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“…Muscle specific miRNAs, including miR-1/206 and miR-133b, are well characterized to play crucial roles in muscle development in human and mouse [ 20 , 21 , 22 , 23 , 24 ]. A previous study demonstrated that miR-206 was involved in the muscling phenotype in growth-selected chicken lines [ 25 ]. However, little is known about miR-206 promoter and its effects on miRNA expression at the transcriptional level in birds.…”

Section: Discussionmentioning

confidence: 99%

Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken

Jia

Lin²,

Abdalla

et al. 2016

IJMS

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miRNAs have been widely investigated in terms of cell proliferation and differentiation. However, little is known about their effects on bird growth. Here we characterized the promoter of miR-206 in chicken and found that the preferable promoter was located in 1200 bp upstream of pri-miR-206. In this region, many key transcription factors, including MyoD, c-Myb, CEBPα/β, AP-4, RAP1, Brn2, GATA-1/2/3, E47, Sn, upstream stimulatory factor (USF) and CdxA, were predicted to bind and interact with miR-206 promoter. Overexpression of MyoD sharply increased miR-206 expression in both fibroblast and myoblast cells, and also the regulation in the myoblast cells was much stronger, indicating that miR-206 was regulated by MyoD combined with other muscle specific transcriptional factors. Aiming to further investigate the relationship between miR-206 mutation and transcriptional expression, total of 23 SNPs were identified in the two distinct bird lines by sequencing. Interestingly, the motif bound by MyoD was individually destroyed by G-to-C mutation located at 419 bp upstream of miR-206 precursor. Co-transfecting MyoD and miR-206 promoter in DF-1 cells, the luciferase activity of promoter containing homozygous GG types was significantly higher than CC ones (p < 0.05). Thus, this mutation caused low expression of miR-206. Consistently, eight variants including G-419C mutation exhibited a great effect on birthweight through maker-trait association analysis in F2 population (p < 0.05). Additionally, the regulation of miR-206 on embryo muscle mass mainly by increasing MyoG and muscle creatine kinase (MCK) expression (p < 0.05) with little change in MyoD, TMEM8C and myosin heavy chain (MHC). In conclusion, our findings provide a novel mutation destroying the promoter activity of miR-206 in birds and shed new light to understand the regulation mechanism of miR-206 on the embryonic muscle growth.

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“…To date, the major progress on the way of clinical utility of miR-221/222 in cardiovascular medicine was achieved in the field of stem cell therapy for cardiovascular diseases. The miR-221/222 cluster is critically involved in the regulation of myogenesis and function of myocardium [ 173 , 182 – 184 ]. Both in myoblasts and myotubules, miR221/222 expression was shown to be under control of the Ras-MAPK pathway and inversely correlated with levels of p27 Kip1 , a common target for these miRNAs [ 182 ].…”

Section: Clinical Potential Of Mir-221/222mentioning

confidence: 99%

Human miR-221/222 in Physiological and Atherosclerotic Vascular Remodeling

Chistiakov

Sobenin

Orekhov

et al. 2015

BioMed Research International

136

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A cluster of miR-221/222 is a key player in vascular biology through exhibiting its effects on vascular smooth muscle cells (VSMCs) and endothelial cells (ECs). These miRNAs contribute to vascular remodeling, an adaptive process involving phenotypic and behavioral changes in vascular cells in response to vascular injury. In proliferative vascular diseases such as atherosclerosis, pathological vascular remodeling plays a prominent role. The miR-221/222 cluster controls development and differentiation of ECs but inhibits their proangiogenic activation, proliferation, and migration. miR-221/222 are primarily implicated in maintaining endothelial integrity and supporting quiescent EC phenotype. Vascular expression of miR-221/222 is upregulated in initial atherogenic stages causing inhibition of angiogenic recruitment of ECs and increasing endothelial dysfunction and EC apoptosis. In contrast, these miRNAs stimulate VSMCs and switching from the VSMC “contractile” phenotype to the “synthetic” phenotype associated with induction of proliferation and motility. In atherosclerotic vessels, miR-221/222 drive neointima formation. Both miRNAs contribute to atherogenic calcification of VSMCs. In advanced plaques, chronic inflammation downregulates miR-221/222 expression in ECs that in turn could activate intralesion neoangiogenesis. In addition, both miRNAs could contribute to cardiovascular pathology through their effects on fat and glucose metabolism in nonvascular tissues such as adipose tissue, liver, and skeletal muscles.

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“…In organisms, a miRNA may act on multiple target genes or multiple miRNAs can regulate a target gene, where these miRNAs form a network that regulates biological activities. Hence, recent research into the functions of miRNAs has focused on the miRNA regulatory networks associated with the formation of animal traits, although most studies have been limited to the identification and expression profiling of miRNAs as well as determining the functions of individual miRNAs . So, we lack an overall understanding of how miRNAs regulate muscle development and fat deposition in chickens.…”

Section: Introductionmentioning

confidence: 99%

Characterization of miRNA transcriptome profiles related to breast muscle development and intramuscular fat deposition in chickens

Zhao

et al. 2018

J of Cellular Biochemistry

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Studies of the miRNA expression profiles associated with the postnatal late development of skeletal muscle and IMF deposition are lacking in chicken. Here, we evaluated the patterns of muscle fiber growth and IMF deposition in breast muscle in the Chinese domestic breed called Gushi chicken, where we constructed four small RNA libraries from breast muscle tissues at 6, 14, 22, and 30 weeks. A total of 388 known miRNAs and 31 novel miRNAs were identified based on four small RNA libraries. Comparative analysis identified 92 significant differentially expressed (SDE) miRNAs based on six combinations. KEGG pathway analysis for the SDE miRNAs showed that metabolic pathways such as glycolysis and biosynthesis of amino acids were significantly enriched before 22 weeks, and pathways such as biosynthesis of unsaturated fatty acids and fatty acid elongation were significantly enriched after 22 weeks. This trend was consistent with the patterns of breast muscle fiber growth and IMF deposition in Gushi chickens. We also constructed miRNA-mRNA interaction networks related to breast muscle development and IMF deposition. The results showed that miRNAs such as gga-miR-1a-3p, and gga-miR-133a-5p may play important roles in breast muscle development, and miRNAs such as gga-miR-103-3p, and gga-miR-138-2-3p may have key roles in IMF deposition. This study determined the dynamic miRNA transcriptome in breast muscle tissue for the first time in Gushi chickens. The results provide a valuable resource for investigating the post-transcriptional regulation mechanisms during postnatal late development of breast muscle and IMF deposition and for evaluating the muscular disease.

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“…However, there have long been controversies about the meat quality and the nutritional value between juvenile and old-aged hens3637. Over the past few years, most studies have focused on the skeletal muscle of chicken before sexual maturation383940. However, until now, the epigenetic mechanisms responsible for breast meat quality between the juvenile and later laying-period hens remained poorly understood.…”

mentioning

confidence: 99%

Genome-wide DNA methylation profiles reveal novel candidate genes associated with meat quality at different age stages in hens

Zhang

Yan

et al. 2017

Sci Rep

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Poultry meat quality is associated with breed, age, tissue and other factors. Many previous studies have focused on distinct breeds; however, little is known regarding the epigenetic regulatory mechanisms in different age stages, such as DNA methylation. Here, we compared the global DNA methylation profiles between juvenile (20 weeks old) and later laying-period (55 weeks old) hens and identified candidate genes related to the development and meat quality of breast muscle using whole-genome bisulfite sequencing. The results showed that the later laying-period hens, which had a higher intramuscular fat (IMF) deposition capacity and water holding capacity (WHC) and less tenderness, exhibited higher global DNA methylation levels than the juvenile hens. A total of 2,714 differentially methylated regions were identified in the present study, which corresponded to 378 differentially methylated genes, mainly affecting muscle development, lipid metabolism, and the ageing process. Hypermethylation of the promoters of the genes ABCA1, COL6A1 and GSTT1L and the resulting transcriptional down-regulation in the later laying-period hens may be the reason for the significant difference in the meat quality between the juvenile and later laying-period hens. These findings contribute to a better understanding of epigenetic regulation in the skeletal muscle development and meat quality of chicken.

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“…miRNAs are a class of small ncRNAs that often negatively regulate gene expression, and some play essential roles in chicken skeletal muscle, e.g., miR-125b, miR-221, and miR-206, miRNA-133a, and miR-1a (Andreote et al 2014;Wang et al 2012). The function of lncRNAs is generally less conserved than that of miRNAs, and lncRNAs act via different mechanisms to play important roles during muscle differentiation.…”

Section: Introductionmentioning

confidence: 99%

Sequencing and characterization of lncRNAs in the breast muscle of Gushi and Arbor Acres chickens

Ren

et al. 2018

Genome

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Chicken muscle quality is one of the most important factors determining the economic value of poultry, and muscle development and growth are affected by genetics, environment, and nutrition. However, little is known about the molecular regulatory mechanisms of long non-coding RNAs (lncRNAs) in chicken skeletal muscle development. Our study aimed to better understand muscle development in chickens and thereby improve meat quality. In this study, Ribo-Zero RNA-Seq was used to investigate differences in the expression profiles of muscle development related genes and associated pathways between Gushi (GS) and Arbor Acres (AA) chickens. We identified two muscle tissue specific expression lncRNAs. In addition, the target genes of these lncRNAs were significantly enriched in certain biological processes and molecular functions, as demonstrated by Gene Ontology (GO) analysis, and these target genes participate in five signaling pathway, as revealed by an analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Taken together, these data suggest that different lncRNAs might be involved in regulating chicken muscle development and growth and provide new insight into the molecular mechanisms of lncRNAs.

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Identification and characterization of microRNAs expressed in chicken skeletal muscle

Cited by 9 publications

References 39 publications

Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken

Human miR-221/222 in Physiological and Atherosclerotic Vascular Remodeling

Characterization of miRNA transcriptome profiles related to breast muscle development and intramuscular fat deposition in chickens

Genome-wide DNA methylation profiles reveal novel candidate genes associated with meat quality at different age stages in hens

Sequencing and characterization of lncRNAs in the breast muscle of Gushi and Arbor Acres chickens

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