Identification and characterization ofSeptoria steviaeas the causal agent of Septoria leaf spot disease of stevia in North Carolina

First report of septoria steviae causing stevia leaf spot in Thailand

“…Koehler et al. (2019) was the first report which used morphology support coupled with multi‐local phylogenetic characters of seven loci including actin (ACT), β‐tubulin (BT), calmodulin (CAL), rDNA internal transcribed spacers (ITS1‐5.8S‐ITS2 = ITS), rDNA 28S subunit (LSU), RNA polymerase II second largest subunit (RPB2) and translation elongation factor‐1α (TEF1) to molecularly confirm species placement of Septoria isolated from stevia. S. steviae clustered as a well‐supported monophyletic group based on molecular characterization and North Carolina isolates were confirmed as S. steviae .…”

“…In 1995, S. steviae was reported in North America based on morphology descriptions of previous reports (Lovering & Reeleder, 1996; Reeleder, 1999). In 2019, Koehler et al identified S. steviae to be the causal agent of stevia leaf spot in the southeastern United States by comparing the morphological and molecular characters of isolates with the ex‐type culture (CBS120132, NBRC31181 and NBRC31190) obtained from the Westerdijk Fungal Biodiversity Institute in the Netherlands and the National Institute of Technology and Evaluation Culture Collection in Japan (Koehler et al., 2019).…”

Identification of three Diaporthe species from Selenicereus sp. explants and their pathogenicity to three dragon fruit species

“…The phylogenetic tree was constructed using the Tamura‐Nei model (Tamura & Nei, 1993) based on the concatenated ITS, TUB2 and TEF1‐α sequences with 1000 bootstraps replicate. Septoria steviae (CBS 120132) was used as the outgroup (Koehler et al, 2019).…”

T-BAS Version 2.1: Tree-Based Alignment Selector Toolkit for Evolutionary Placement of DNA Sequences and Viewing Alignments and Specimen Metadata on Curated and Custom Trees

“…1). Phylogeny-based placement of DNA sequences can be performed on trees and multilocus alignments (e.g., those available in TreeBase [www.treebase.org] [4, 5] or custom trees uploaded by users), greatly expanding the utility of T-BAS across biological disciplines. Trees, alignments, and specimen metadata also can be uploaded and viewed without performing placements (6, 7), making it a versatile phylogenetic tool across diverse taxonomic groups.…”