Identification and characterization of heavy metal-resistant bacteria selected from different polluted sources

Mounaouer, Brahmi; Nesrine, Achour; Hassen, Abdennaceur

doi:10.1080/19443994.2013.823565

Cited by 24 publications

(12 citation statements)

References 32 publications

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“…Gram-positive bacteria have a thick layer of peptidoglycan (20-80 nm) in which they fix acids, proteins, and polysaccharides. This layer forms a barrier to avoid the entry of heavy metals into the cell, prevailing passive resistance mechanisms such as adsorption (Bruins et al 2000;Mounaouer et al 2014). In contrast, Gramnegative bacteria have thinner walls (5-10 nm) that allow the influx and efflux of heavy elements and force the bacteria to develop active mechanisms or flow systems and to have a higher protein synthesis in presence of Cd (Bruins et al 2000).…”

Section: Culturable Of Cd-rb Isolation Morphological Characterization and Molecular Identificationmentioning

confidence: 99%

Assessment of native cadmium-resistant bacteria in cacao (Theobroma cacao L.) - cultivated soils

Cordoba-Novoa

Cáceres-Zambrano

Torres‐Rojas

2021

Preprint

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Traces of cadmium (Cd) have been reported in some chocolate products due to soils with Cd and the high ability of cacao plants to extract, transport, and accumulate it in their tissues. An agronomic strategy to minimize the uptake of Cd by plants is the use of cadmium-resistant bacteria (Cd-RB). However, knowledge about Cd-RB associated with cacao soils is scarce. This study was aimed to isolate and characterize Cd-RB associated with cacao-cultivated soils in Colombia that may be used in the bioremediation of Cd-polluted soils. Diversity of culturable Cd-RB, qualitative functional analysis related to nitrogen, phosphorous, carbon, and Cd were performed. Thirty different Cd-RB morphotypes were isolated from soils with medium (NC, Y1, Y2) and high (Y3) Cd concentrations using culture media with 6 mg Kg-1 Cd. Cd-RB were identified based on morphological and molecular analyses. The most abundant morphotypes (90%) were gram-negative belong to Phylum Proteobacteria and almost half of them showed the capacity to fix nitrogen, solubilize phosphates and degrade cellulose. Unique morphotypes were isolated from Y3 soils where Burkholderia and Pseudomonas were the dominant genera indicating their capacity to resist high Cd concentrations. P. putida GB78, P. aeruginosa NB2, and Burkholderia sp. NB10 were the only morphotypes that grew on 18 up to 90 (GB78) and 140 mg Kg-1 Cd (NB2-NB10); however, GB78 showed the highest Cd bioaccumulation (5.92 mg g-1). This study provides novel information about culturable Cd-RB soil diversity with the potential to develop biotechnology-based strategies.

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Section: Culturable Of Cd-rb Isolation Morphological Characterization and Molecular Identificationmentioning

confidence: 99%

Assessment of native cadmium-resistant bacteria in cacao (Theobroma cacao L.) - cultivated soils

Cordoba-Novoa

Cáceres-Zambrano

Torres‐Rojas

2021

Preprint

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“…Bacteria have developed coping mechanisms for toxic heavy metal levels. Gram negative bacteria can be advantageous against heavy metals (Mounaouer et al 2014). The more positive correlation of the metals with Gram positive bacteria in this study can be explained by the cell wall structure difference.…”

Section: Bacteriummentioning

confidence: 56%

The Correlation of Some Nutrient Elements and Antibacterial Activity of the Basil (Ocimum basilicum)

Eser

Adiloğlu

2020

Tekirdağ Ziraat Fakültesi Dergisi

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This study aimed to analyze the correlation of some macro and micro nutritional elements of the basil plant (Ocimum basilicum) with the antibacterial activity of the basil extract on two Gram positive and two Gram negative bacteria. The extract of the basil plants grown in laboratory conditions was obtained by solid-liquid extraction with ethanol after drying and burning of the freshly harvested above ground parts. The macro (P, K, Ca, and Mg), micro (Fe, Cu, Zn, Mn, and B) nutrients and a functional element (Al) amounts were detected by ICP-OES. The results revealed that the basil extract was compatible with herbaceous perennial plants in terms of macro nutrient elements P, K, Ca and Mg. Moreover, some micronutrients (Fe, Mn, Zn, B) and the functional element Al was found to be significantly high. The basil extract was significantly effective on inhibiting the Gram negative E. coli, P. aeruginosa, and Gram positive S. aureus and E. faecium. The element B was found to be positively correlated with the antibacterial activity of the basil plant suggesting as the B content increases, the antibacterial activity of basil extract is supposed to increase, too. The P and K contents were found to be positively correlated with antibacterial activity, while Ca and Mg contents were negatively correlated. The correlation analysis of the nutritional elements, this extract can be suggested to be more preferrable against Gram positive bacteria, especially when the Fe, Cu, Zn, Mn levels are high. These results revealed the importance of the macro and micro nutrient contents of medicinal plants which can be used traditionally against various diseases. In basil cultivation the soils low in lime may convert the negative correlation of Ca and Mg into a positive correlation in terms of antibacterial effect. Conscious and proper fertilization therefore is suggested to be effective on antibacterial activity of the basil plant.

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“…Mounaouer et al . [ 49 ] demonstrated that among the heavy-metal-tolerant Gram-positive ( S. aureus ) and Gram-negative ( P. aeruginosa ) bacterial isolates from wastewater samples, P. aeruginosa was found to be more resistant to a number of heavy metals (MIC based order of toxicity: Cu >Cr> Cd>Ni> Zn>Co), while the Gram-negative isolates harboured a single plasmid. Antibiogram analysis, as demonstrated by Kaur et al .…”

Section: Discussionmentioning

confidence: 99%

Principal component analysis exploring the association between antibiotic resistance and heavy metal tolerance of plasmid-bearing sewage wastewater bacteria of clinical relevance

Mandal

Das

Mandal

2020

Access Microbiology

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This paper unravels the occurrence of plasmid-mediated antibiotic resistance in association with tolerance to heavy metals among clinically relevant bacteria isolated from sewage wastewater. The bacteria isolated were identified following conventional phenotypic and/or molecular methods, and were subjected to multiple-antibiotic resistance (MAR) profiling. The isolates were tested against the heavy metals Hg 2+ , Cd 2+ , Cr 2+ and Cu 2+ . SDS-PAGE and agarose gel electrophoretic analyses were performed, respectively, for the characterization of heavy metal stress protein and R-plasmid among the isolated bacteria. Principal component analysis was applied in determining bacterial resistance to antibiotics and heavy metals. Both lactose-fermenting ( Escherichia coli ) and non-fermenting ( Acinetobacter baumannii and Pseudomonas putida ) Gram-negative bacterial strains were procured, and showed MAR phenotypes with respect to three or more antibiotics, along with resistance to the heavy metals Hg 2+ , Cd 2+ , Cr 2+ and Cu 2+ . The Gram-positive bacteria, Enterococcus faecalis , isolated had ‘ampicillin–kanamycin–nalidixic acid’ resistance. The bacterial isolates had MAR indices of 0.3–0.9, indicating their ( E. faecalis , E. coli , A. baumannii and P. putida ) origin from niches with high antibiotic pollution and human faecal contamination. The Gram-negative bacteria isolated contained a single plasmid (≈54 kb) conferring multiple antibiotic resistance, which was linked to heavy metal tolerance; the SDS-PAGE analysis demonstrated the expression of heavy metal stress proteins (≈59 and ≈10 kDa) in wastewater bacteria with a Cd 2+ stressor. The study results grant an insight into the co-occurrence of antibiotic resistance and heavy metal tolerance among clinically relevant bacteria in sewage wastewater, prompting an intense health impact over antibiotic usage.

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Identification and characterization of heavy metal-resistant bacteria selected from different polluted sources

Cited by 24 publications

References 32 publications

Assessment of native cadmium-resistant bacteria in cacao (Theobroma cacao L.) - cultivated soils

Assessment of native cadmium-resistant bacteria in cacao (Theobroma cacao L.) - cultivated soils

The Correlation of Some Nutrient Elements and Antibacterial Activity of the Basil (Ocimum basilicum)

Principal component analysis exploring the association between antibiotic resistance and heavy metal tolerance of plasmid-bearing sewage wastewater bacteria of clinical relevance

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