Identification and Characterization of Antagonism Band of Secondary Metabolite from T. asperellum MK045610 against F. oxysporum f. sp. ciceri and F. oxysporum f. sp. lycopersici based on HPTLC and GC-MS

Abstract

<jats:p>The present investigation was carried out on identification and characterization of an antagonism band of extract secondary metabolites from Trichoderma asperellum MK045610 against Fusarium oxysporum f. sp. ciceri and Fusarium oxysporum f. sp. lycopersici. In this experiment, analysis was performed by High Performance Thin Layer Chromatography (HPTLC), paper disc assay, gas chromatography-Mass spectrometry (GC-MS). Firstly, extract crude secondary metabolites were used for partial purification based on HPTLC. Secondly, the paper disc assay method was used for the determination of antifungal property on PDA plate from the partial purified compound. Thirdly, GC-MS was used for identification of partial purified compound based on peaks. Identified compounds are named as Phenol, 3, 5-bis (1,1-dimethylethyl), Pentadecanoic Acid, 14-methyl, methyl ester, Benzenepropanoic acid, 3,5,-bis (1,1-dimethyl ethyl)-4-hydroxy-methyl ester and represented to antifungal property . Conclusively, Secondary metabolite of Trichoderma asperellum MK045610 has a significant role in radial growth inhibition of Fusarium oxysporum f. sp. ciceri and Fusarium oxysporum f. sp. lycopersici.</jats:p>