Identification and Characterization of a Garlic Virus E Genome in Garlic (Allium sativum L.) Using High-Throughput Sequencing from India

Prajapati, Malyaj R.; Manav, Aakansha; Singh, Jitender; Kumar, Pankaj; Kumar, Amit; Kumar, Ravindra; Prakash, Satya

doi:10.3390/plants11020224

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…The use of new massively parallel sequencing technologies facilitates the identification of viruses in plants showing symptoms of virosis. The Illumina technology, for example, has been used to detect Garlic virus E [ 144 ].…”

Section: Pathogens Of Garlic: Threats Treatments and Perspectivesmentioning

confidence: 99%

Turning Garlic into a Modern Crop: State of the Art and Perspectives

Parreño

Rodríguez-Alcocer

Martínez-Guardiola

et al. 2023

Plants

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Garlic is cultivated worldwide for the value of its bulbs, but its cultivation is challenged by the infertility of commercial cultivars and the accumulation of pathogens over time, which occurs as a consequence of vegetative (clonal) propagation. In this review, we summarize the state of the art of garlic genetics and genomics, highlighting recent developments that will lead to its development as a modern crop, including the restoration of sexual reproduction in some garlic strains. The set of tools available to the breeder currently includes a chromosome-scale assembly of the garlic genome and multiple transcriptome assemblies that are furthering our understanding of the molecular processes underlying important traits like the infertility, the induction of flowering and bulbing, the organoleptic properties and resistance to various pathogens.

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Section: Pathogens Of Garlic: Threats Treatments and Perspectivesmentioning

confidence: 99%

Turning Garlic into a Modern Crop: State of the Art and Perspectives

Parreño

Rodríguez-Alcocer

Martínez-Guardiola

et al. 2023

Plants

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“…It is a pungent bulbous crop that originates from central Asia. Its applicability extends to culinary and therapeutic purposes where it has been utilized as a traditional medicine for the treatment of various ailments (Prajapati et al, 2022). In the present day, the cultivation of garlic continues to thrive, notably in the regions of Asia, the Mediterranean, and North and South America.…”

Section: Allium Sativum Lmentioning

confidence: 99%

Kitchen Spices as Complementary and Alternative Medicine: Pharmacothera¬peutic Potential and Challenges

Iqbal,

Aslam,

Sahar

et al. 2023

Complementary and Alternative Medicine: One Health Perspective

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Complementary and alternative medicine (CAM) recognizes the health advantages of a variety of kitchen spices, including celery, lemongrass, cardamom, saffron, cloves, garlic, cumin, cinnamon, turmeric, ginger, fenugreek, and cloves. They endow them with humor. Garlic's antimicrobial qualities are well-known for supporting heart health and thwarting illness. Because clove is an analgesic, anti-inflammatory, and nerve-healthy herb, it helps reduce pain. The antioxidant potential of Cumin can also aid in weight management and better digestion. Cinnamon which possesses anti-inflammatory and anti-diabetic qualities could support heart health and blood sugar regulation. Additionally, it treats a number of illnesses and eases joint discomfort. Ginger has anti-inflammatory and digestive properties which aid in the relief of constipation. Besides helping with blood sugar regulation, fenugreek is beneficial for nursing moms. Among the many health benefits of celery is its ability to lower inflammation and indigestion. Lemongrass is well known for its antibacterial properties, which can also reduce tension and aid with digestion. Cardamom can help with digestion and improve oral health. Saffron is widely recognized for enhancing mental health, elevating mood, and reducing symptoms of depression. These spices have many medical properties that enhance the therapeutic role of food in rituals. When combined with a balanced diet, they can provide a delicious and comprehensive way to preserve health and well-being. Before using it medicinally, you should speak with a doctor, especially if you have any specific health issues or plan to use it in conjunction with other prescriptions.

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“…Reads with low quality, indicated by a Phred score of 30 or lower, and reads containing ambiguous bases "N" were eliminated. Adaptor sequences with low-quality bases at the 5' and 3' ends were trimmed to enhance data quality [7]. Subsequently, the ltered and clean data were subjected to de novo assembly using CLC Workbench 20.0.4 software, followed by quality control (QC) to ensure the integrity and reliability of the results.…”

Section: Data Processing and De Novo Transcripts Assemblymentioning

confidence: 99%

De novo assembly and annotation of Hellula undalis (Fabr.) (Lepidoptera: Pyralidae) transcriptome

Prajapati

Kumar

Shanker

et al. 2023

Preprint

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Background The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a significant pest of brassicas and other cruciferous plants in warm regions worldwide. Transcriptome analysis is valuable for investigation of molecular mechanisms underlying the insect development and reproduction. De novo assembly is particularly useful for acquiring complete transcriptome information of insect species when there is no reference genome available. In case of Hellula undalis, only 17 nucleotide records are currently available throughout NCBI nucleotide database. Genes associated with metabolic processes, general development, reproduction, defense and functional genomics were not previously predicted in the Hellula undalis at the genomic level. Methods & Results To address this issue, we constructed Hellula undalis transcriptome using Illumina NovaSeq6000 technology. Approximately 48 million 125 bp paired-end reads were obtained from sequencing. A total of 30,451 contigs were generated by de novo assembly of sample and were compared with the sequences in the NCBI non-redundant protein database (Nr). In total, 71% of contigs were matched to known proteins in public databases including Nr, Gene Ontology (GO), and Cluster Orthologous Gene Database (COG), and then, contigs were mapped to 123 via functional annotation against the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG). In addition, we compared the ortholog gene family of the Hullula undalis, transcriptome to Spodoptera frugiperda, spodotera litura and spodoptera littoralis and found that 2,749 orthologous gene families are specific to Hullula undalis. Conclusions This study is the first transcriptome data for Hullula undalis. Additionally, it serves as a valuable resource for identifying target genes and developing effective and environmentally friendly strategies for pest control.

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Identification and Characterization of a Garlic Virus E Genome in Garlic (Allium sativum L.) Using High-Throughput Sequencing from India

Cited by 8 publications

References 27 publications

Turning Garlic into a Modern Crop: State of the Art and Perspectives

Turning Garlic into a Modern Crop: State of the Art and Perspectives

Kitchen Spices as Complementary and Alternative Medicine: Pharmacothera¬peutic Potential and Challenges

De novo assembly and annotation of Hellula undalis (Fabr.) (Lepidoptera: Pyralidae) transcriptome

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