Identification and characterisation of an early/late bi-directional promoter of the capripoxvirus, lumpy skin disease virus

Fick, W; Viljoen, G. J.

doi:10.1007/s007050050582

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…Current molecular data on the LSDV genome consists of restriction endonuclease analysis, cross-hybridization studies, and limited transcriptional and DNA sequence analysis (5,19,20,26,27,33). Given the economic significance of LSD, its potential for spread into nonenzootic regions, and the interest in developing more effective LSDV-based vaccines and expression vectors, we have sequenced and analyzed the genome of a pathogenic LSDV.…”

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confidence: 99%

Genome of Lumpy Skin Disease Virus

Tulman

Afonso

et al. 2001

J Virol

322

250

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Lumpy skin disease virus (LSDV), a member of the capripoxvirus genus of the Poxviridae, is the etiologic agent of an important disease of cattle in Africa. Here we report the genomic sequence of LSDV. The 151-kbp LSDV genome consists of a central coding region bounded by identical 2.4 kbp-inverted terminal repeats and contains 156 putative genes. Comparison of LSDV with chordopoxviruses of other genera reveals 146 conserved genes which encode proteins involved in transcription and mRNA biogenesis, nucleotide metabolism, DNA replication, protein processing, virion structure and assembly, and viral virulence and host range. In the central genomic region, LSDV genes share a high degree of colinearity and amino acid identity (average of 65%) with genes of other known mammalian poxviruses, particularly suipoxvirus, yatapoxvirus, and leporipoxviruses. In the terminal regions, colinearity is disrupted and poxvirus homologues are either absent or share a lower percentage of amino acid identity (average of 43%). Most of these differences involve genes and gene families with likely functions involving viral virulence and host range. Although LSDV resembles leporipoxviruses in gene content and organization, it also contains homologues of interleukin-10 (IL-10), IL-1 binding proteins, G protein-coupled CC chemokine receptor, and epidermal growth factor-like protein which are found in other poxvirus genera. These data show that although LSDV is closely related to other members of the Chordopoxvirinae, it contains a unique complement of genes responsible for viral host range and virulence.

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confidence: 99%

Genome of Lumpy Skin Disease Virus

Tulman

Afonso

et al. 2001

J Virol

322

250

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“…Previously, the use of GTPV as a vaccine vector has been reported in China and abroad, and numerous ruminant mammal protective antigens have been expressed in the GTPV vaccine vector expression system, which have demonstrated promising results (17,18). The present study selected VVI1L promoter-expressed exogenous genes as VVI1L is a strong promoter, as its activity is 10-fold greater than that of VVP7.5 (10,12).…”

Section: Discussionmentioning

confidence: 99%

Expression and functional analysis of Brucella outer membrane protein 25 in recombinant goat pox virus

et al. 2019

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The Capripoxvir us (CaPV) has a large double-stranded DNA genome and a restricted host-range. At present, it is being investigated as an ideal vaccine vector. In the present study, a novel recombinant goat pox virus (rGTPV) was constructed to express Brucella outer membrane protein (OMP)25, and was validated by in vitro and in vivo immunization assays. A novel rGTPV vector was created, in which the thymidine kinase gene was used as a flanking sequence, I1L was inserted as a promoter element to enhance Brucella OMP25 expression, and p7.5 as another promoter element was used to regulate guanine phosphoribosyl-transferase as a selection maker. The rGTPV vector was transfected into sheep fetal fibroblast/lamb testis cells pre-infected with GTPV G14-STV44-55 to recombine. Brucella OMP25 protein was expressed in cells by rGTPV, and activated immune reactivity to Brucella OMP25 protein, as detected by western blotting. Furthermore, rGTPV elicited, anti-Brucella-specific immunoglobulin G responses, as determined by ELISA. Mice vaccinated with rGTPV did not exhibit pathology alterations in the kidney and liver. These results suggested that the novel rGTPV was able to efficiently drive Brucella OMP25 protein expression and activate immune reactivity, and may have applications in CaPV live vector vaccines and associated research.

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“…While sequencing the genome, a 42 nucleotide (nt) sequence was identified that seemed unusually well conserved; unusual in both its length and the fact that it was almost perfectly conserved between members of four different poxvirus genera. Although subsequent experiments on the CSE ultimately led to its classification as a promoter element in poxviruses [10], the CSE remains unusual because it is remarkably well conserved for a promoter, it is longer than the average poxvirus promoter (which is normally in the range of ∼30 nts) [11], and it actually contains both early and late promoter elements that are believed to act on an early/late gene [11,12]. The presence of both early and late promoter elements, as well as the very high conservation of the CSE, makes it more complex than other characterized promoters [10,13–14].…”

Section: Introductionmentioning

confidence: 99%

JaPaFi: A Novel Program for the Identification of Highly Conserved DNA Sequences

Sadeque

Barsky²,

Marass³

et al. 2010

Viruses

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We describe the use of Java Pattern Finder (JaPaFi) to identify short (<100 nt) highly conserved sequences in a series of poxvirus genomes. The algorithm utilizes pattern matching to identify approximate matches appearing at least once in each member of a set of genomes; a key feature is that the genomes do not need to be aligned. The user simply specifies the genomes to search, minimum length of sequences to find and the maximum number of mismatches and indels allowed. Many of the most highly conserved segments contain poxvirus promoter elements.

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Identification and characterisation of an early/late bi-directional promoter of the capripoxvirus, lumpy skin disease virus

Cited by 5 publications

References 29 publications

Genome of Lumpy Skin Disease Virus

Genome of Lumpy Skin Disease Virus

Expression and functional analysis of Brucella outer membrane protein 25 in recombinant goat pox virus

JaPaFi: A Novel Program for the Identification of Highly Conserved DNA Sequences

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