Identification and antimicrobial susceptibility of Corynebacterium pseudotuberculosis isolated from sheep

Caseous lymphadenitis is still a serious zoonotic problem in Turkey. Sheep suffer from the disease with yield loss in wool and meat production. Moreover, with inexperienced laboratory staff, biochemical identification may go unrevealed. The scope of this study was to demonstrate the presence of Corynebacterium pseudotuberculosis in sheep by PCR. The sampling was conducted via collecting lymph fluids from the lymph node internal pouch wall of 100 sheep that were examined for the presence of Corynebacterium pseudotuberculosis. Molecular identification of the Corynebacterium pseudotuberculosis isolates was carried out by establishing the presence of the proline iminopeptidase gene. All isolates were confirmed to be Corynebacterium pseudotuberculosis by polymerase chain reaction. The polymerase chain reaction procedure conducted in this research was observed to be reliable and fast, and could be utilized for confirmation of caseous lymphadenitis in sheep as an optional technique to timeconsuming biochemical identification methods. Pleomorphic bacteria, caseous lymphadenitis, proline iminopeptidase, PCR

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Molecular identification of Corynebacterium pseudotuberculosis in sheep

Parın

Kırkan

Ural

et al. 2018

Acta Vet. Brno

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Açlık Stresine Maruz Kalan Corynebacterium pseudotuberculosis’in SDS-PAGE İle Proteinlerinin Analizi

Demir,

Erdenlig Gürbilek

2023

Harran Üniversitesi Veteriner Fakültesi Dergisi

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Corynebacterium pseudotuberculosis, hücre içi bir patojen olup küçük ruminantlarda kazeöz lenfadenit (KLA) hastalığına sebep olan, Gram-pozitif fakültatif bir bakteridir. Corynebacterium pseudotuberculosis, insanlarda ve hayvanlarda hastalık oluşturan bir bakteri türüdür. Bu bakteri, açlık stresine maruz kaldığında, hücresel yapısını ve metabolizmasını değiştirerek adaptasyon gösterir. Bu değişiklikler, bakterinin protein ekspresyonunda da değişikliklere neden olabilir. Bu çalışmada da, Corynebacterium pseudotuberculosis’in stres faktörlerine maruz bırakıldığında ve genel besiyerlerinde üretildiğinde protein profillerinde nasıl bir farklılık olacağı araştırılmıştır. SDS-PAGE analizi, açlık stresine maruz kalan Corynebacterium pseudotuberculosis'teki protein ekspresyonundaki değişiklikleri incelemek için kullanılabilir. Bu analiz, bakterinin strese karşı adaptasyonunu ve bu adaptasyonun enfeksiyonun yayılmasına nasıl katkıda bulunduğunu daha iyi anlamamıza yardımcı olabilir. Bu araştırmada SDS-PAGE analizi ile izole edilen ve referans olarak kullanılan Corynebacterium pseudotuberculosis suşlarının Tryptic Soy Broth ve RPMI 1640 (Roswell Park Memorial Institute) besiyerlerinde üretilip strese maruz bırakılan suşun protein profilleri incelenmiştir. Sonuç olarak, strese maruz bırakılan Corynebacterium suşları arasında protein yapıları arasında profil farklılıkları olduğu tespit edilmiştir.

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The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye

Tarhane,

Büyük

2024

Life

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Sheep caseous lymphadenitis (CLA) causes significant economic losses in the livestock sector by causing a loss in the quantity and quality of animal products and a loss in the breeding value of animals. Although the primary agent in CLA’s etiology is Corynebacterium pseudotuberculosis, some other opportunistic microorganisms also play a role. Therefore, the control and treatment of CLA necessitates the identification of the relevant etiological agents. This study aimed to conduct an in vitro culture and molecular characterization (PCR analysis and 16S rRNA sequencing) of the bacteria involved in sheep CLA cases reported in the Çankırı province of Türkiye and determine the antibiotic susceptibility of the case isolates. In total, 82 (16.4%) of 500 sheep in five farms were diagnosed with CLA. Following the culture of the superficial abscesses samples, C. pseudotuberculosis was identified in 30 (36.59%) as a result of PCR, Pseudomonas spp. in 8 (9.76%), and Enterobacter cancerogenus in 1 (1.22%), as a result of 16S rRNA sequencing. These data revealed extensive heterogeneity among the Pseudomonas isolates, with hints of derivation from a common ancestry for some and phylogenetic similarity to isolates from Germany, Malaysia, and India. In contrast to the high susceptibility to cefoperazone and lincomycin, the high resistances of C. pseudotuberculosis and Pseudomonas spp. isolates to cephalothin, ceftiofur, cloxacillin, amoxicillin, and bacitracin were remarkable. Based on these findings, it was concluded that for an effective treatment and control of ovine CLA cases, there is a need to consider the possible involvement of opportunistic bacteria other than the primary causative agent, C. pseudotuberculosis. It also contributed to increasing the country-specific sequence data and establishing new taxa from a universal perspective.

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Identification and antimicrobial susceptibility of Corynebacterium pseudotuberculosis isolated from sheep

Cited by 3 publications

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Molecular identification of Corynebacterium pseudotuberculosis in sheep

Molecular identification of Corynebacterium pseudotuberculosis in sheep

Açlık Stresine Maruz Kalan Corynebacterium pseudotuberculosis’in SDS-PAGE İle Proteinlerinin Analizi

The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye

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