Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil

Abstract

The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candiduria admitted to the University Hospital of the Federal University of Mato Grosso do Sul were evaluated. Both, species identification and susceptibility testing with fluconazole-FLC, voriconazole-VRC, and amphotericin B-AmB were carried out using the Vitek 2. To discriminate species of the C. parapsilosis complex, a RAPD-PCR technique using the RPO2 primer was performed. From the total of 106 isolates, 42 (39.6%) C. albicans and 64 (60.4%) Candida non-albicans (CNA) - 33 C. tropicalis, 18 C. glabrata, 5 C. krusei, 4 C. parapsilosis sensu stricto, 2 C. kefyr, 1 C. lusitaniae, and 1 C. guilliermondii were identified. All isolates were susceptible to AmB and VRC, whereas all C. glabrata isolates presented either resistance (5.6%) or dose-dependent susceptibility (94.4%) to FLC. The study of Candida spp. and their resistance profiles may help in tailoring more efficient therapeutic strategies for candiduria.

Candida Urinary Tract Infection Among ICU Patients in Isfahan, Iran

“…We followed up all patients especially diabetic patients (44%) and fortunately nobody presented systemic candidiasis in the present investigation. Similar to the present study, females usually have a higher chance for candiduria and UTI (24) in connection with vulvovaginal colonization by Candida species and their anatomy (6,25), however, in many investigations males are the predominant population among UTI patients (11,26). Candida species are isolated from 20-60% of UTI in neonatal intensive care units (NICU) and pediatric intensive care units (PICU) (27), but in the present study, the frequency of UTI among infants was only 1%.…”

“…Since older individuals reveal natural modifications of the immune system, so longer hospitalization in intensive care units and use of urinary catheters was seen more commonly in this population (28,29). Similar to most surveys in this field (6,27), most patients in the present investigation were over 65 years old. The median absolute neutrophil count (ANC) less than 1.5 × 10 9 /L is considered as neutropenia (30,31).…”

“…Candida species present in the urinary tract via the climbing route, from the urethra to the bladder, and hematogenous dissemination by filtration of Candida spp. in kidneys and excretion to the urine (6). Urinary tract instrumentation, prolonged hospitalized stay, widespread antibiotic consumption, extremes of age, corticosteroid therapy, organ transplantation, female gender, diabetes mellitus, and use of immunosuppressive agents are the most predisposing factors (7).…”

Antifungal Resistance of Candida Species in Bacolod City, Philippines

“…Frequency on Candida infection was most notably observed on respiratory specimens and predominantly in older adults. Aside from the environmental and cultural factors [17], the most probable reasons for this condition are host-related factors such as underlying malignancies, immunosuppressive diseases, hematopoietic stem cell or organ transplantation, frequent use of wide-spectrum antibiotics, invasive interventions, chemotherapy, parenteral alimentation, use of internal prosthetic devices, natural modification of the immune system [3], age, neutropenia, and deteriorating clinical conditions due to underlying disease [1,18].…”

“…The study was based on the paper of Tan, et al [8], Lima, et al [3], and Bhattacharjee [9]. This retrospective observational study was performed in the microbiology section of a tertiary hospital in Bacolod City from July 2017 to July 2018.…”