Identification and Antifungal Activity of Compounds from the Mangrove Endophytic Fungus Aspergillus clavatus R7

Abstract

Two new coumarin derivatives, 4,4′-dimethoxy-5,5′-dimethyl-7,7′-oxydicoumarin (1), 7-(γ,γ-dimethylallyloxy)-5-methoxy-4-methylcoumarin (2), a new chromone derivative, (S)-5-hydroxy-2,6-dimethyl-4H-furo[3,4-g]benzopyran-4,8(6H)-dione (5), and a new sterone derivative, 24-hydroxylergosta-4,6,8(14),22-tetraen-3-one (6), along with two known bicoumarins, kotanin (3) and orlandin (4), were isolated from an endophytic fungus Aspergillus clavatus (collection No. R7), isolated from the root of Myoporum bontioides collected from Leizhou Peninsula, China. Their structures were elucidated using 1D- and 2D- NMR spectroscopy, and HRESIMS. The absolute configuration of compound 5 was determined by comparison of the experimental and calculated electronic circular dichroism (ECD) spectra. Compound 6 significantly inhibited the plant pathogenic fungi Fusarium oxysporum, Colletotrichum musae and Penicillium italicum, compound 5 significantly inhibited Colletotrichum musae, and compounds 1, 3 and 4 greatly inhibited Fusarium oxysporum, showing the antifungal activities higher than those of the positive control, triadimefon.

Marine natural products

“…286 An Ascomycota sp. yielded enantiomeric polyketide dimers 740 and 741, 287 whilst Aspergillus strains were the source of coumarin derivatives 742, 743, 288 chromone 744, 288 sterone 745, 288 anhydride derivative 746, 289 lipid amide 747, 289 xanthonoids 748-751 and derivative 752, 290 meroterpenoid 753 (ref. 291) and chromanone 754.…”

Produksi Pigmen dan Identifikasi Kapang Penghasilnya Menggunakan Pendekatan DNA Barcoding

“…Beberapa peneliti berhasil mengisolasi senyawa aktif dari kapang endofit laut yang diisolasi dari berbagai tumbuhan pesisir di antaranya vaccinols J dari Pestalotiopsis vaccinii dengan aktivitas sebagai anti-enterovirus (EV71), phochrodines D memiliki aktivitas sebagai antioksidan yang diisolasi dari Phomopsis sp. 33#, kontanin dan orlandin sebagai antijamur yang diisolasi kapang Aspergillus clavatus R7 Li et al 2017;Wang et al 2017). Hutan mangrove Indonesia menyimpan biodiversitas kapang endofit yang belum banyak dikaji.…”

Design, synthesis, antifungal activity, and 3D-QSAR of coumarin derivatives

“…They exhibit a broad spectrum of biological activities, including anticancer, antioxidant, antiplasmodial, antimalarial, antivirus, antibacterial, and antifungal. [1][2][3][4][5][6][7] The varied pharmacological activities and relatively simple structures of these compounds have spurred great interest in their possible therapeutic uses. In addition to coumarin's use as the lead compound for the preparation of potent pharmacological molecules, it has also received considerable research attention for its interesting insecticidal, nematicidal, allelopathic, and antimicrobial activities in agriculture.…”