Iberian allele frequency database for 10 X-STRs

Baeta, Miriam; Illescas, María José; García, Luis Fernando; Núñez, Carolina; Prieto-Fernández, Endika; Jiménez-Moreno, Susana; Pancorbo, Marian M. de

doi:10.1016/j.fsigen.2015.06.009

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…A total of 101 European Caucasoid profiles were compared with the previously obtained genotypes by using the decaplex of the GHEP‐ISFG . A complete concordance was observed.…”

Section: Resultsmentioning

confidence: 77%

“…The concordance of the X‐STR loci DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08, and DXS7423 included in the 17 X‐STR panel was established by comparison to the European Caucasoid population profiles ( N = 101) obtained with the decaplex of the GHEP‐ISFG in a previous study carried out in our laboratory . Additionally, positive controls (2800M, 9947A, and K562) were used to test the concordance of the other seven markers (DXS6799, DXS6800, DXS6801, DXS6803, DX6807, DXS10075, and DXS10079).…”

Section: Methodsmentioning

confidence: 99%

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Development of a new highly efficient 17 X‐STR multiplex for forensic purposes

et al. 2016

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Currently, two of the most widely used X-chromosome STR (X-STR) multiplexes are composed by ten (GHEP-ISFG decaplex) and 12 markers (Investigator Argus X-12 Kit). The number of markers included is a drawback for complex relative testing cases, likewise the large size of some amplicons difficult their application to degraded samples. Here, we present a new multiplex of 17 X-STRs with the aim of increasing both the resolution power and forensic applicability. This newly proposed set includes the X-STRs of the GHEP-ISFG decaplex, four X-STRs from the Investigator Argus X-12 Kit, three of them also included in the decaplex, and six additional more. In order to ensure the allele designation, an allelic ladder was developed. The validation of the present multiplex was carried out according to the revised guidelines by the SWGDAM (Scientific Working Group on DNA Analysis Methods). A total of 488 unrelated individuals from four different continents were analyzed. The forensic efficiency evaluation showed high values of combined power of discrimination in males (≥0.999999996) and females (≥0.999999999999995) as well as combined paternity exclusion probabilities in trios (≥0.99999998) and duos (≥0.999996). The results presented herein have demonstrated that the new 17 X-STR set constitutes a high-resolution alternative to the current X-STR multiplexes.

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“…A total of 101 European Caucasoid profiles were compared with the previously obtained genotypes by using the decaplex of the GHEP‐ISFG . A complete concordance was observed.…”

Section: Resultsmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Development of a new highly efficient 17 X‐STR multiplex for forensic purposes

et al. 2016

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“…Statistical kinship calculations based on Likelihood ratios (LRs) for autosomal STRs were carried out using GFF v.2.55 [9], using allele frequencies of the Spanish population (GHEP Collaborative Exercise, 2014), and for X-STRs an Iberian frequency database was applied [10]. Y chromosome and mtDNA LRs were calculated based on haplotype frequencies obtained in the YHRD and EMPOP databases.…”

Section: Methodsmentioning

confidence: 99%

A grave in my garden. Genetic identification of Spanish civil war victims buried in two mass graves in Espinosa de los Monteros (Burgos, Spain)

Núñez

Baeta

Palencia-Madrid

et al. 2015

Forensic Science International: Genetics Supplement Series

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A B S T R A C TWe report the genetic study of 13 victims of the Spanish Civil war buried in two mass graves in Espinosa de los Monteros (Burgos, Spain). The grave with the highest number of individuals (grave 1; N = 9) was found in the garden of a private house of the village. The other grave (grave 2) was only 200 m away and the remains of four women were unexpectedly found. Skeletal remains, mainly teeth, were better preserved in graves 1 than 2. Mean DNA extraction yield was 0.17 ng/ml. In order to carry out the genetic identification of the remains, buccal swabs from 11 family relatives were available. The choice of genetic markers analyzed relied on the different levels of biological kinship to be elucidated. For 75% of the postmortem samples, !12 autosomal STR profiles were obtained. Y chromosome STRs, as well as mitochondrial DNA control region, were also analyzed in order to study paternal and maternal lineages, respectively. Besides, X chromosome STRs were typed in one case to verify a paternal grandmothergranddaughter relationship. We successfully identified 9 of 13 individuals buried in the two mass graves of Espinosa de los Monteros, 6 in grave 1 and 3 in grave 2. The search of further relatives' samples would be of interest to perform more comparative analyses, and eventually do not leave any of the victims without a name.

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