α-Amylase andα-Glucosidase Inhibitory Saponins fromPolyscias fruticosaLeaves

Hạnh, Trần Thị Hồng; Dang, Nguyen Hai; Dat, Nguyen Tien

doi:10.1155/2016/2082946

Cited by 29 publications

(21 citation statements)

References 12 publications

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“…The yeast -glucosidase (G0660, Sigma-Aldrich) inhibition assay was performed using the substrate p-nitrophenyl--D-glucopyranoside according to the previously described method [12]. Briefly, the sample solution (2 L dissolved in DMSO) and 0.5 U/mL -glucosidase (40 L) were mixed in 120 L of 0.1 M phosphate buffer (pH 7.0).…”

Section: Assay For -Glucosidase Inhibitionmentioning

confidence: 99%

Chemical Composition andα-Glucosidase Inhibitory Activity of VietnameseCitrusPeels Essential Oils

Dang

Nhung

Anh

et al. 2016

Journal of Chemistry

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Background. Inhibition ofα-glucosidase is an important factor to control postprandial hyperglycemia in type 2 diabetes mellitus.Citrusessential oils (CEO) are among the most widely used essential oils, and some of them exhibited promising antidiabetic effect. However, theα-glucosidase inhibition of CEO has not been investigated so far. The present work aims to evaluate theα-glucosidase inhibition of essential oils from six VietnameseCitruspeels.Methods. The chemical composition of essential oils obtained by hydrodistillation from sixCitruspeels was analyzed by GC-MS. All essential oils were tested for their inhibitory activity onα-glucosidase usingp-nitrophenyl-α-D-glucopyranoside as substrate.Results. In Buddha’s hand and lime peels, the major components were limonene (59.0–61.31%) andγ-terpinene (13.98–23.84%) while limonene (90.95–95.74%) was most abundant in pomelo, orange, tangerine, and calamondin peels. Among the essential oils, the Buddha’s hand oil showed the most significantα-glucosidase inhibitory effect with the IC50value of 412.2 μg/mL. The combination of the Buddha’s hand essential oil and the antidiabetic drug acarbose increased the inhibitory effect.Conclusions. The results suggested the potential use of Buddha’s hand essential oil as an alternative in treatment of type 2 diabetes mellitus.

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Section: Assay For -Glucosidase Inhibitionmentioning

confidence: 99%

Chemical Composition andα-Glucosidase Inhibitory Activity of VietnameseCitrusPeels Essential Oils

Dang

Nhung

Anh

et al. 2016

Journal of Chemistry

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“…Especially important, given the high NCD (particularly diabetes) rates in the Pacific and northern Australia, are the anti-diabetes and anti-cardiovascular disease effects of most of the plants featured in the factsheets, demonstrated in scientific studies. Studies with evidence for this include the following: drumstick [ 36 , 37 , 38 , 50 , 60 ], amaranth [ 61 , 62 , 63 ], bele [ 64 , 65 , 66 ], chilli [ 67 , 68 , 69 ], purslane [ 70 , 71 , 72 ], kangkong [ 73 , 74 , 75 ], ofenga [ 76 , 77 , 78 ], hedge panax [ 79 , 80 ], chaya [ 39 , 40 ], pumpkin [ 81 , 82 ], and choko [ 83 , 84 ]. Their inclusion in the diet in sufficient quantity is likely to reduce the risk of diabetes and cardiovascular disease, not only by reducing glycemic load when they are included with high-carbohydrate meals, but also because of specific anti-diabetes effects.…”

Section: Resultsmentioning

confidence: 99%

Macro- and Micronutrients from Traditional Food Plants Could Improve Nutrition and Reduce Non-Communicable Diseases of Islanders on Atolls in the South Pacific

Lyons

Dean

Tongaiaba³

et al. 2020

Plants

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Pacific Islanders have paid dearly for abandoning traditional diets, with diabetes and other non-communicable diseases (NCD) widespread. Starchy root crops like sweet potato, taro, and cassava are difficult to grow on the potassium-deficient soils of atolls, and high energy, low nutrient imported foods and drinks are popular. Nutritious, leafy food plants adapted to alkaline, salty, coral soils could form part of a food system strategy to reduce NCD rates. This project targeted four atolls south of Tarawa, Kiribati, and was later extended to Tuvalu. Mineral levels in diverse, local leafy food plants were compared to reveal genotype–environment interactions. Food plants varied in ability to accumulate minerals in leaves and in tolerance of mineral-deficient soils. Awareness activities which included agriculture, health, and education officers targeted atoll communities. Agriculture staff grew planting material in nurseries and provided it to farmers. Rejuvenation of abandoned giant swamp taro pits to form diversified nutritious food gardens was encouraged. Factsheets promoted the most suitable species from 24 analyzed, with multiple samples of each. These included Cnidoscolus aconitifolius (chaya), Pseuderanthemum whartonianum (ofenga), Polyscias scutellaria (hedge panax), and Portulaca oleracea (purslane). The promoted plants have been shown in other studies to have anti-NCD effects. Inclusion of the findings in school curricula and practical application in the form of demonstration school food gardens, as well as increased uptake by farmers, are needed. Further research is needed on bioavailability of minerals in plants containing phytates and tannins.

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“…The ethanol extract of N. fruticosus leaves also had AChE inhibitor activity with 22.8% ± 0.3% inhibition at 1,000 µg/ml. Three bisdesmosidic saponins were isolated from the methanol extract of N. fruticosus leaves (Hanh et al, 2016). Other studies in vivo reported that saponin compounds which were extracted from Fenugreek seeds (Trigonella foenum-graecum) increased the AChE inhibitor in Alzheimer's-induced rats (Khalil et al, 2016).…”

Section: Total Phenolic and Flavonoid Contentsmentioning

confidence: 99%

Acetylcholinesterase: Inhibitory activity of some Indonesian vegetables and fraction of selected plants

2020

J app pharm sci

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Dementia is a global health problem that requires severe treatment. People with dementia who consume vegetables every day can reduce the risk of dementia. Alzheimer's is one of the diseases that are characterized by dementia, which results in decreased neurotransmitter acetylcholine. This study was to investigate the acetylcholinesterase (AChE) enzyme inhibitory activity of various vegetables that are widely used by Indonesian Sundanese and Javanese ethnics. In this study, total phenolic and flavonoid content were analyzed for the determination of active compounds of the extracts. Ethanol extracts of 13 vegetables have been tested for AChE inhibitory activity using Ellman's colorimetric method in 96-well plate. Four types of extracts (Cosmos caudatus, Nasturtium officinale, Nothopanax fruticosus, and Ocimum americanum) had higher total phenolic and flavonoid content than other extracts. Vegetable extracts that had IC 50 values of less than 1,000 µg/ml were C. caudatus and O. americanum. Both of these extracts were partitioned with n-hexane and water. The IC 50 values of water fraction of C. caudatus and n-hexane fraction of O. americanum were 325.0 ± 18.3 and 374.4 ± 42.1 µg/ml, respectively. These results showed that fractions had a potential inhibitor of AChE, and the chemical components (phenolic, flavonoid, and terpenoid) can be isolated to find the active compound.

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α-Amylase andα-Glucosidase Inhibitory Saponins fromPolyscias fruticosaLeaves

Cited by 29 publications

References 12 publications

Chemical Composition andα-Glucosidase Inhibitory Activity of VietnameseCitrusPeels Essential Oils

Chemical Composition andα-Glucosidase Inhibitory Activity of VietnameseCitrusPeels Essential Oils

Macro- and Micronutrients from Traditional Food Plants Could Improve Nutrition and Reduce Non-Communicable Diseases of Islanders on Atolls in the South Pacific

Acetylcholinesterase: Inhibitory activity of some Indonesian vegetables and fraction of selected plants

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