2009
DOI: 10.1242/dev.034637
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Abstract: In the nematode Caenorhabditis elegans, sex is determined by the ratio of X chromosomes to sets of autosomes: XX animals (2X:2A1.0) develop as hermaphrodites and XO animals (1X:2A0.5) develop as males. TRA-1, the worm ortholog of Drosophila Cubitus interruptus and mammalian Gli (Glioma-associated homolog) proteins, is the terminal transcription factor of the C. elegans sex-determination pathway, which specifies hermaphrodite fate by repressing male-specific genes. Here we identify a consensus TRA-1 binding s… Show more

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Cited by 20 publications
(25 citation statements)
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“…One of these consensus TRA‐1‐binding sites is located at 3‐kilobase (kb) upstream of the daf‐16d/f isoforms, while the other is located within the first exon of the daf‐16a isoform (exonic sequences often serve as binding elements for transcriptions factors; Stergachis et al., 2013). The ChIP‐seq analysis provided by Berkseth and colleagues also identified two potential TRA‐1‐binding sites in the daf‐16 coding region (Berkseth et al., 2013) which are however slightly diverged from the canonical one (Conradt & Horvitz, 1999; Hargitai et al., 2009) and the daf‐16 ‐specific TRA‐1‐binding sites identified by our present study (Figure 2a). To assess the functionality of these potential TRA‐1‐binding sites, we generated two transgenic strains expressing isoform‐specific gfp ‐ (green fluorescent protein) tagged daf‐16 reporter constructs, daf‐16d/f::gfp and daf‐16a::gfp (Figure 2a).…”
Section: Resultssupporting
confidence: 63%
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“…One of these consensus TRA‐1‐binding sites is located at 3‐kilobase (kb) upstream of the daf‐16d/f isoforms, while the other is located within the first exon of the daf‐16a isoform (exonic sequences often serve as binding elements for transcriptions factors; Stergachis et al., 2013). The ChIP‐seq analysis provided by Berkseth and colleagues also identified two potential TRA‐1‐binding sites in the daf‐16 coding region (Berkseth et al., 2013) which are however slightly diverged from the canonical one (Conradt & Horvitz, 1999; Hargitai et al., 2009) and the daf‐16 ‐specific TRA‐1‐binding sites identified by our present study (Figure 2a). To assess the functionality of these potential TRA‐1‐binding sites, we generated two transgenic strains expressing isoform‐specific gfp ‐ (green fluorescent protein) tagged daf‐16 reporter constructs, daf‐16d/f::gfp and daf‐16a::gfp (Figure 2a).…”
Section: Resultssupporting
confidence: 63%
“…In other words, TRA‐1 strengthens the function of daf‐16 in aging control, explaining why hermaphrodites live significantly longer than males in populations containing both sexes. At first sight, these results were somewhat unexpected as TRA‐1 had previously been known as a transcriptional repressor rather than an activator (Berkseth et al., 2013; Chen & Ellis, 2000; Conradt & Horvitz, 1999; Hargitai et al., 2009; Mason et al., 2008; Schwartz & Horvitz, 2007; Szabó et al., 2009; Yi et al., 2000). In case of mammalian GLI proteins, however, both activation and inhibition functions were observed (Hui & Angers, 2011).…”
Section: Resultsmentioning
confidence: 99%
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“…Second, we observed apparent feedback on upstream sex determination genes. TRA-1 can bind to a site in the xol-1 promoter in vitro and regulates xol-1 reporter transgenes via this site (27). ChIP-seq confirmed in vivo TRA-1 binding to this site (Fig.…”
Section: Tra-1 Feeds Back Onto the Sex Determination Pathway At Multiplementioning
confidence: 66%