<i>Wax-deficient anther1</i> Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development

Jung, Ki Hong; Han, Min Young; Lee, Dong Yeun; Lee, Yang-Seok; Schreiber, Lukas; Franke, Rochus; Faust, Andréa; Yephremov, Alexander; Saedler, Heinz; Kim, Young Kook; Hwang, Inhwan; An, Gynheung

doi:10.1105/tpc.106.042044

Cited by 241 publications

(244 citation statements)

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“…One such quantitative trait locus, panicle apical abortion8, affects the formation of anther and pollen, and level of reactive oxygen species (ROS) in spikelets (Cheng et al, 2011). Several reports also indicate that cuticle deficiency can cause degenerated anthers and abnormal pollen grains (Jung et al, 2006;Li et al, 2010). Cuticles, the hydrophobic, waxy structures produced by the epidermal cells in most land plants, play an important role in plant development and in the tolerance to environmental stresses.…”

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confidence: 99%

Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

et al. 2015

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Panicle development, a key event in rice (Oryza sativa) reproduction and a critical determinant of grain yield, forms a branched structure containing multiple spikelets. Genetic and environmental factors can perturb panicle development, causing panicles to degenerate and producing characteristic whitish, small spikelets with severely reduced fertility and yield; however, little is known about the molecular basis of the formation of degenerating panicles in rice. Here, we report the identification and characterization of the rice panicle degenerative mutant tutou1 (tut1), which shows severe defects in panicle development. The tut1 also shows a pleiotropic phenotype, characterized by short roots, reduced plant height, and abnormal development of anthers and pollen grains. Molecular genetic studies revealed that TUT1 encodes a suppressor of cAMP receptor/Wiskott-Aldrich syndrome protein family verprolin-homologous (SCAR/WAVE)-like protein. We found that TUT1 contains conserved functional domains found in eukaryotic SCAR/WAVE proteins, and was able to activate Actin-related protein2/3 to promote actin nucleation and polymerization in vitro. Consistently, tut1 mutants show defects in the arrangement of actin filaments in trichome. These results indicate that TUT1 is a functional SCAR/WAVE protein and plays an important role in panicle development.

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confidence: 99%

Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

et al. 2015

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“…The primers used for RT-PCR are described in Table 2. Actin 1 (Os03g50890; NCBI accession number OSJNBa0078A17.12) and Ubq1 (Os03g13170; NCBI accession number OJ1175C11.2) were used as controls for RT-PCR [11].…”

Section: Analysis Of Alternatively Spliced Transcriptmentioning

confidence: 99%

Analysis of Alternatively Spliced Rice Transcripts Using Microarray Data

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Bartley

Cao

et al. 2008

Rice

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Alternative splicing creates a diversity of gene products in higher eukaryotes. Twenty-five percent (1,583/ 6,371) of predicted alternatively spliced transcripts can be detected using the NSF45K rice whole-genome oligonucleotide array. We used the NSF45K array to assess differential expression patterns of 507 loci showing at least a twofold change in expression between light-and darkgrown seedlings. At least 42% of these loci show evidence of alternative splicing in aerial seedling tissue of Oryza sativa ssp. japonica cv. Nipponbare. Most alternative splice forms display the same pattern of regulation as the primary, or most highly expressed, transcript; however, splice forms for ten loci, represented by 35 oligos, display opposite expression patterns in the light vs. dark. We found similar evidence of alternative splicing events in Affymetrix microarray data for Nipponbare rice treated with the causative agent of fungal rice blast, Magnaporthe grisea. This strategy for analyzing alternative splicing in microarray data will enable delineation of the diversity of splicing in rice.

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“…This may be due to the fact that amylose constitutes only up to ∼20% of the total starch, and mutants with altered amylose may not necessarily result in significant changes in total starch. Some mutations are found in genes involved in the individual metabolism pathway, suggesting that seed quality can be regulated by directly modifying the genes involved in the synthesis or metabolism pathways of starch, fat and so on [41][42][43]. Additionally, other genes fall into many other diverse molecular function categories and some genes are associated with more than one changed constituents of seeds, indicating the close relationship between the regulation of synthesis and metabolism of these constituents.…”

Section: Genes Associated With Seed Quality Control Are Involved In Dmentioning

confidence: 99%

“…go.jp/~miyao/pub/tos17/index.html.en) [35,36], Korea (http://141.223.132.44/pfg/) [37,38] and USA (http:// sundarlab.ucdavis.edu/) [39]. These resources helped to identify and study genes involved in rice development [40,41]. Some mutants with marked changes of seed quality were also identified [42,43].…”

Section: Fang-fang Fu Et Al 381mentioning

confidence: 99%

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Studies on rice seed quality through analysis of a large-scale T-DNA insertion population

et al. 2009

Cell Res

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A rice (Oryza sativa) T-DNA insertion population, which included more than 63 000 independent transgenic lines and 8 840 identified flanking sequence tags (FSTs) that were mapped onto the rice genome, was developed to systemically study the rice seed quality control. Genome-wide analysis of the FST distribution showed that T-DNA insertions were positively correlated with expressed genes, but negatively with transposable elements and small RNAs. In addition, the recovered T-DNAs were preferentially located at the untranslated region of the expressed genes. More than 11 000 putative homozygous lines were obtained through multi-generations of planting and resistance screening, and measurement of seed quality of around half of them, including the contents of starch, amylose, protein and fat, with a nondestructive near-infrared spectroscopy method, identified 551 mutants with unique or multiple altered parameters of seed quality. Analysis of the corresponding FSTs showed that genes participating in diverse functions, including metabolic processes and transcriptional regulation, were involved, indicating that seed quality is regulated by a complex network. IntroductionRice (Oryza sativa), as a wholesome and nutritious cereal grain, provides the staple food for more than half of the world's population [1]. Along with the growth of economies and population throughout the world, there is an increasing demand for high-yield and high-quality rice. The grain quality of rice, including that of milling, appearance, cooking and eating, and nutrition, is determined by multiple physicochemical properties [2], and studies on the involved key genes and relevant regulatory mechanisms will help to illustrate the regulatory networks of rice quality control and benefit for the breeding efforts.Starch, which is composed of amylose and amylopectin, comprises ∼90% of dry endosperm of rice seed.The apparent amylose content (AAC) is recognized as an important determinant of the appearance and texture of grain [3]. Thai jasmine rice (KDML 105), one of the best-quality rice with good cooking and eating qualities, has low amylose content (AC) and medium gel consistency (GC) [4].The nutritional quality includes the proportions of protein, fat, mineral and other substances [5]. The rice protein is rich in methionine and cysteine, and compares favorably with proteins of other cereals, but is poor in lysine and threonine, making it an incomplete protein source for human infants [6]. Storage triacylglycerols are stored in oil bodies containing phospholipids and oleosins at the surface [7], and oil bodies are abundant in embryo and aleurone layer of rice seed. However, the aleurone layer is usually removed by milling because it turns rancid upon storage [8]. In addition, oil also influences other aspects of seed quality, and a recent report showed that the quality and viability of Arabidopsis seeds can be enhanced by suppressing phospholipase D [9].The near-infrared spectroscopy (NIRS) technology, which is based on the fact that several natural pr...

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Wax-deficient anther1 Is Involved in Cuticle and Wax Production in Rice Anther Walls and Is Required for Pollen Development

Cited by 241 publications

References 59 publications

Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

Analysis of Alternatively Spliced Rice Transcripts Using Microarray Data

Studies on rice seed quality through analysis of a large-scale T-DNA insertion population

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