<i>Trichoderma atroviride</i>‐emitted volatiles improve growth of <i>Arabidopsis</i> seedlings through modulation of sucrose transport and metabolism

Esparza‐Reynoso, Saraí; Ruíz-Herrera, León Francisco; Pelagio‐Flores, Ramón; Macías‐Rodríguez, Lourdes; Martı́nez-Trujillo, Miguel; López-Coria, Montserrat; Sánchez‐Nieto, Sobeida; Herrera-Estrella, Alfredo; López‐Bucio, José

doi:10.1111/pce.14014

Cited by 38 publications

(23 citation statements)

References 89 publications

(165 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Especially, increasing evidences showed that endophyte can directly and indirectly promote the growth and secondary metabolites of its host plants through various ways [ 12 – 14 ]. For example, endophyte can induced the development of its host plants by directly producing plant growth hormone themselves [ 12 ], or indirectly by promoting its host plants capacity of nutrients absorption and stress resistance [ 13 ]. Furthermore, endophyte can also produce bioactive compounds which are the same or similar to the secondary metabolites in its hosts [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Endophytic fungus Pseudodidymocyrtis lobariellae KL27 promotes taxol biosynthesis and accumulation in Taxus chinensis

Cao

Wang

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

Background Taxol from Taxus species is a precious drug used for the treatment of cancer and can effectively inhibit the proliferation of cancer cells. However, the growth of Taxus plants is very slow and the content of taxol is quite low. Therefore, it is of great significance to improve the yield of taxol by modern biotechnology without destroying the wild forest resources. Endophytic fungus which symbiosis with their host plants can promote the growth and secondary metabolism of medicinal plants. Results Here, an endophytic fungus KL27 was isolated from T. chinensis, and identified as Pseudodidymocyrtis lobariellae. The fermentation broth of KL27 (KL27-FB) could significantly promote the accumulation of taxol in needles of T. chinensis, reaching 0.361 ± 0.082 mg/g·DW (dry weight) at 7 days after KL27-FB treatment, which is 3.26-fold increase as compared to the control. The RNA-seq and qRT-PCR showed that KL27-FB could significantly increase the expression of key genes involved in the upstream pathway of terpene synthesis (such as DXS and DXR) and those in the taxol biosynthesis pathway (such as GGPPS, TS, T5OH, TAT, T10OH, T14OH, T2OH, TBT, DBAT and PAM), especially at the early stage of the stimulation. Moreover, the activation of jasmonic acid (JA) biosynthesis and JA signal transduction, and its crosstalk with other hormones, such as gibberellin acid (GA), ethylene (ET) and salicylic acid (SA), explained the elevation of most of the differential expressed genes related to taxol biosynthesis pathway. Moreover, TF (transcriptional factor)-encoding genes, including MYBs, ethylene-responsive transcription factors (ERFs) and basic/helix-loop-helix (bHLH), were detected as differential expressed genes after KL27-FB treatment, further suggested that the regulation of hormone signaling on genes of taxol biosynthesis was mediated by TFs. Conclusions Our results indicated that fermentation broth of endophytic fungus KL27-FB could effectively enhance the accumulation of taxol in T. chinensis needles by regulating the phytohormone metabolism and signal transduction and further up-regulating the expression of multiple key genes involved in taxol biosynthesis. This study provides new insight into the regulatory mechanism of how endophytic fungus promotes the production and accumulation of taxol in Taxus sp.

show abstract

Section: Introductionmentioning

confidence: 99%

Endophytic fungus Pseudodidymocyrtis lobariellae KL27 promotes taxol biosynthesis and accumulation in Taxus chinensis

Cao

Wang

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…The many secondary metabolites produced by Trichoderma species are responsible for their highly antagonistic effects, contributing to the success of their application [ 12 ]. Additionally, the application of Trichoderma species successfully regulates root architecture, increasing the length of lateral and primary roots, leading to the more efficient uptake of nutrients by the plant, resulting in biostimulation [ 13 – 15 ]. The mechanism through which Trichoderma exerts biostimulatory effects involves multilevel communication with root and shoot systems, including releasing many active metabolites into the rhizosphere, thereby increasing plant growth and yield [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…These findings indicate that VOCs may play a role in the biostimulatory and biocontrol activities of Trichoderma spp. [ 13 , 17 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma atroviride LZ42 releases volatile organic compounds promoting plant growth and suppressing Fusarium wilt disease in tomato seedlings

Rao¹,

Zeng²,

Jiang³

et al. 2022

BMC Microbiol

View full text Add to dashboard Cite

Background The promotion of plant growth and suppression of plant disease using beneficial microorganisms is considered an alternative to the application of chemical fertilizers or pesticides in the field. Results A coconut-scented antagonistic Trichoderma strain LZ42, previously isolated from Ganoderma lucidum-cultivated soil, was investigated for biostimulatory and biocontrol functions in tomato seedlings. Morphological and phylogenetic analyses suggested that strain LZ42 is closely related to T. atroviride. Tomato seedlings showed increased aerial and root dry weights in greenhouse trials after treatment with T. atroviride LZ42 formulated in talc, indicating the biostimulatory function of this fungus. T. atroviride LZ42 effectively suppressed Fusarium wilt disease in tomato seedlings, with an 82.69% control efficiency, which is similar to that of the carbendazim treatment. The volatile organic compounds (VOCs) emitted by T. atroviride LZ42 were found to affect the primary root growth direction and promote the root growth of tomato seedlings in root Y-tube olfactometer assays. The fungal VOCs from T. atroviride LZ42 were observed to significantly inhibit F. oxysporum in a sandwiched Petri dish assay. SPME–GC–MS analysis revealed several VOCs emitted by T. atroviride LZ42; the dominant compound was tentatively identified as 6-pentyl-2H-pyran-2-one (6-PP). The VOC 6-PP exhibited a stronger ability to influence the direction of the primary roots of tomato seedlings but not the length of the primary roots. The inhibitory effect of 6-PP on F. oxysporum was the highest among the tested pure VOCs, showing a 50% effective concentration (EC50) of 5.76 μL mL−1 headspace. Conclusions Trichoderma atroviride LZ42, which emits VOCs with multiple functions, is a promising agent for the biostimulation of vegetable plants and integrated management of Fusarium wilt disease.

show abstract

“…The many secondary metabolites produced by Trichoderma species are responsible for their highly antagonistic effects, contributing to the success of their application [11]. Additionally, the application of Trichoderma species successfully regulates root architecture, increasing the length of lateral and primary roots, leading to the more efficient uptake of nutrients by the plant, resulting in biostimulation [12][13][14]. The mechanism through which Trichoderma exerts biostimulatory effects involves multilevel communication with root and shoot systems, including releasing many active metabolites into the rhizosphere, thereby increasing plant growth and yield [2].…”

Section: Introductionmentioning

confidence: 99%

“…These findings indicate that VOCs may play a role in the biostimulatory and biocontrol activities of Trichoderma spp. [12,16,25].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma atroviride LZ42 Releases Volatile Organic Compounds Promoting Plant Growth and Resisting Fusarium wilt Disease in Tomatoes

Jiang¹,

Rao²,

Zeng³

et al. 2021

Preprint

View full text Add to dashboard Cite

The promotion of plant growth and suppression of plant disease using beneficial microorganisms is considered an alternative to the application of chemical fertilizers or pesticides in the field. In this study, a coconut-scented antagonistic Trichoderma strain LZ42, previously isolated from Genoderma lucidum-cultivated soil, was investigated for biostimulatory and biocontrol functions in tomato seedlings. Morphological and phylogenetic analyses suggested that strain LZ42 is closely related to T. atroviride. Tomato plants showed increased aerial and root dry weights in greenhouse trials after treatment with T. atroviride LZ42 formulated in talc, indicating the biostimulatory function of this fungus. T. atroviride LZ42 effectively suppressed Fusarium wilt disease in tomato seedlings, with an 82.69% control efficiency, which is similar to that of fungicide treatment. The volatile organic compounds (VOCs) emitted by T. atroviride LZ42 were found to affect the primary root growth direction and promote the root growth of tomato seedlings in root Y-tube olfactometer assays. The fungal VOCs from T. atroviride LZ42 were observed to significantly inhibit F. oxysporum in a sandwiched Petri dish assay. SPME-GC-MS analysis revealed several VOCs emitted by T. atroviride LZ42; the dominant compound was tentatively identified as 6-pentyl-2H-pyran-2-one (6-PP). 6-PP exhibited a stronger ability to influence the direction of the primary roots of tomato seedlings but not the length of the primary roots. The inhibitory effect of 6-PP on F. oxysporum was the highest among the tested pure VOCs, showing a 50% effective concentration (EC50) of 5.76 μL mL-1 headspace. In conclusion, T. atroviride LZ42, which emits VOCs with multiple functions, is a promising agent for the biostimulation of vegetable plants and integrated management of Fusarium wilt disease.

show abstract

Trichoderma atroviride‐emitted volatiles improve growth of Arabidopsis seedlings through modulation of sucrose transport and metabolism

Cited by 38 publications

References 89 publications

Endophytic fungus Pseudodidymocyrtis lobariellae KL27 promotes taxol biosynthesis and accumulation in Taxus chinensis

Endophytic fungus Pseudodidymocyrtis lobariellae KL27 promotes taxol biosynthesis and accumulation in Taxus chinensis

Trichoderma atroviride LZ42 releases volatile organic compounds promoting plant growth and suppressing Fusarium wilt disease in tomato seedlings

Trichoderma atroviride LZ42 Releases Volatile Organic Compounds Promoting Plant Growth and Resisting Fusarium wilt Disease in Tomatoes

Contact Info

Product

Resources

About