STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice

Abstract

SUMMARYFloral organ identity is defined by organ homoetic genes whose coordinated expression is crucial with respect to the time and place of floral organ formation. Here, we report molecular cloning and characterization of the rice STAMENLESS 1 (SL1) gene that is involved in floral development. The sl1 mutant largely resembles the rice B-class gene mutant spw1; both exhibit homeotic conversions of lodicules and stamens to palea/lemmalike organs and carpels. Additionally, sl1 produces flowers with varied numbers of inner floral organs, and amorphous tissues without floral organ identity were frequently formed in whorls 3 and 4. We also show that SL1 specifies lodicule and stamen identities through positive transcriptional regulation of SPW1/OsMADS16 expression. SL1 encodes a member of the C2H2 family of zinc finger proteins, closely related to JAG of Arabidopsis. The functional divergence between SL1 and JAG implies that SL1 was co-opted for its distinctive roles in specification of floral organ identity in rice after the lineage split from Arabidopsis.

A homolog of Blade-On-Petiole 1 and 2 (BOP1/2) controls internode length and homeotic changes of the barley inflorescence

“…Both TF classes regulate KNOX gene expression in a shared pathway together with AtBOP1/2 and are repressed in Arabidopsis leaves and bracts (Norberg et al, 2005;Ha et al, 2007;Jun et al, 2010). Mutations of members belonging to these TF families are associated with changes in the spatiotemporal expression of KNOX genes causing pleiotropic alterations in the rice inflorescences: (1) elongated panicles (Horigome et al, 2009); (2) reduced growth of the lemma and palea; and (3) alterations in the number and identity of floral organs (Horigome et al, 2009;Xiao et al, 2009;Tanaka et al, 2012). These analogies in rice and barley anatomy and the similarities in the regulatory networks among rice and Arabidopsis may again imply that BOP gene regulatory pathways are partially conserved between monocot and dicot plants, suggesting that the barley BOP-like gene HvLax-a could fulfill a central function in controlling meristem identity, as BOP1/2 does in Arabidopsis.…”

MULTI-FLORET SPIKELET1, Which Encodes an AP2/ERF Protein, Determines Spikelet Meristem Fate and Sterile Lemma Identity in Rice

“…The SL1ORF-GFP protein acted as a positive nuclear gene control (Xiao et al, 2009). Green fluorescence was detected in the nuclei of rice protoplasts for both MFS1ORF-GFP and SL1ORF-GFP fusion proteins (Fig.…”

Respuesta transcripcional de Populus a bifenilos policlorados: Aspectos aplicados a la fitorremediación

“…Esta estrategia presenta ventajas frente al uso de microarrays. En primer lugar, tiene mayor sensibilidad y reproductibilidad, además de requerir menor cantidad de RNA de partida (Wang et al, 2009;Oshlack et al, 2010, Chen et al, 2011. En segundo lugar, permite capturar un mayor nñumero de tránscritos expresados.…”

“…En tercer lugar, genera información no solo de la secuencia, sino también de la estructura de exones y posibles eventos de splicing alternativo (Lister et al, 2009;Gulledge et al, 2012). Por si fuera por los datos arrojados por RNA-seq tienen en general una precisión alta, comparable a los estudios de expresión génica que se obtienen a través de qRT-PCR (Nagalakshmi et al, 2008;Wang et al, 2009;Ward et al, 2012).…”

“…Esto significa que la cantidad de lecturas obtenidas es suficiente para detectar los transcritos diferencialmente expresados según lo establecido por Tarazona et al, (2011). La calidad de nuestras librerías fue también corroborada por la distribución de las lecturas sobre los genes de referencia referida también como cobertura (Wang et al, 2009;Sims et al, 2014).…”