<i>Silene vulgaris</i> subsp. <i>macrocarpa</i> leaves and roots from Morocco: assessment of the efficiency of different extraction techniques and solvents on their antioxidant capacity, brine shrimp toxicity and phenolic characterization

Mohti, Hicham; Taviano, Maria Fernanda; Cacciola, Francesco; Dugo, Paola; Mondello, Luigi; Zaîd, Abdelhamid; Cavò, Emilia; Miceli, Natalizia

doi:10.1080/11263504.2019.1674404

Cited by 12 publications

(8 citation statements)

References 31 publications

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“…The total phenolic contents of B. incana extracts were determined by the Folin-Ciocalteau method compared to the calibration curve of gallic acid phenol compound used as a standard [54]. The extracts were dissolved in 70% MeOH; to 100 µL of each sample solution, 0.2 mL Folin-Ciocalteu reagent, 2 mL of H 2 O, and 1 mL of 15% Na 2 CO 3 were added.…”

Section: Determination Of Total Phenolic Contentmentioning

confidence: 99%

“…The free radical scavenging activity of B. incana extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test [54]. Different amounts of each extract were dissolved in 70% MeOH to obtain final concentrations in the range of 0.0625-2 mg/mL.…”

Section: Free Radical Scavenging Activitymentioning

confidence: 99%

“…The protective effects of B. incana extracts on bacterial growth and survival from the oxidative stress induced by hydrogen peroxide (H 2 O 2 ) were evaluated in Escherichia coli ATCC 25922 [54]. The strain was obtained from the Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, with in-house culture collection (Messina, Italy).…”

Section: Protective Effect On Escherichia Coli Growth and Survival Unmentioning

confidence: 99%

“…The toxic potential of B. incana extracts was investigated in brine shrimp (Artemia salina Leach) [54]. Ten brine shrimp larvae, taken 48 h after initiation of hatching in artificial seawater, were transferred to each sample vial, then artificial seawater was added to obtain a final volume of 5 mL.…”

Section: Brine Shrimp Lethality Bioassaymentioning

confidence: 99%

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Brassica incana Ten. (Brassicaceae): Phenolic Constituents, Antioxidant and Cytotoxic Properties of the Leaf and Flowering Top Extracts

et al. 2020

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Brassica incana Ten. is an edible plant belonging to the Brassicaceae family. In this work, the phenolic composition and the antioxidant and cytotoxic properties of the hydroalcoholic extracts obtained from the leaves and the flowering tops of B. incana grown wild in Sicily (Italy) were studied for the first time. A total of 17 and 20 polyphenolic compounds were identified in the leaf and in the flowering top extracts, respectively, by HPLC-PDA-ESI-MS analysis. Brassica incana extracts showed in vitro antioxidant properties; the leaf extract displayed greater radical scavenging activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test than the flowering top extract (IC50 = 1.306 ± 0.049 mg/mL and 2.077 ± 0.011 mg/mL), which in turn had a stronger ferrous ion chelating ability than the other (IC50 = 0.232 ± 0.002 mg/mL and 1.147 ± 0.016 mg/mL). The cytotoxicity of the extracts against human colorectal adenocarcinoma (CaCo-2) and breast cancer (MCF-7) cell lines was evaluated through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the lactic dehydrogenase (LDH) release determination. The extracts showed cytotoxic efficacy against Caco-2 cells, with the flowering top extract being the most effective (about 90% activity at the highest concentration tested). In the brine shrimp lethality bioassay, the extracts exhibited no toxicity, indicating their potential safety.

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Section: Determination Of Total Phenolic Contentmentioning

confidence: 99%

Section: Free Radical Scavenging Activitymentioning

confidence: 99%

Section: Protective Effect On Escherichia Coli Growth and Survival Unmentioning

confidence: 99%

Section: Brine Shrimp Lethality Bioassaymentioning

confidence: 99%

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Brassica incana Ten. (Brassicaceae): Phenolic Constituents, Antioxidant and Cytotoxic Properties of the Leaf and Flowering Top Extracts

et al. 2020

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“…The content of condensed tannin in the A. absinthium extracts was determined by the vanillin method according to the instructions of Mohti et al [ 36 ]. First, 50 μL of the extract solution was mixed with 1.5 mL vanillin (MeOH, 4%) and 750 μL of concentrated acid (HCl) was added.…”

Section: Methodsmentioning

confidence: 99%

Artemisia absinthium L. Aqueous and Ethyl Acetate Extracts: Antioxidant Effect and Potential Activity In Vitro and In Vivo against Pancreatic α-Amylase and Intestinal α-Glucosidase

et al. 2022

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Artemisia absinthium L. is one of the plants which has been used in folk medicine for many diseases over many centuries. This study aims to analyze the chemical composition of the Artemisia absinthium ethyl acetate and its aqueous extracts and to evaluate their effect on the pancreatic α-amylase enzyme and the intestinal α-glucosidase enzyme. In this study, the total contents of phenolic compounds, flavonoids, and condensed tannins in ethyl acetate and the aqueous extracts of Artemisia absinthium leaves were determined by using spectrophotometric techniques, then the antioxidant capacity of these extracts was examined using three methods, namely, the DPPH (2, 2-diphenyl-1picrylhydrazyl) free radical scavenging method, the iron reduction method FRAP, and the β-carotene bleaching method. The determination of the chemical composition of the extracts was carried out using high-performance liquid chromatography—the photodiode array detector (HPLC-DAD). These extracts were also evaluated for their ability to inhibit the activity of the pancreatic α-amylase enzyme, as well as the intestinal α-glucosidase enzyme, in vitro and in vivo, thus causing the reduction of blood glucose. The results of this study showed that high polyphenol and flavonoid contents were obtained in ethyl acetate extract with values of 60.34 ± 0.43 mg GAE/g and 25.842 ± 0.241 mg QE/g, respectively, compared to the aqueous extract. The results indicated that the aqueous extract had a higher condensed tannin content (3.070 ± 0.022 mg EC/g) than the ethyl acetate extract (0.987 ± 0.078 mg EC/g). Ethyl acetate extract showed good DPPH radical scavenging and iron reduction FRAP activity, with an IC50 of 0.167 ± 0.004 mg/mL and 0.923 ± 0.0283 mg/mL, respectively. The β-carotene test indicated that the aqueous and ethyl acetate extracts were able to delay the decoloration of β-carotene with an inhibition of 48.7% and 48.3%, respectively, which may mean that the extracts have antioxidant activity. HPLC analysis revealed the presence of naringenin and caffeic acid as major products in AQE and EAE, respectively. Indeed, this study showed that the aqueous and ethyl acetate extracts significantly inhibited the pancreatic α-amylase and intestinal α-glucosidase, in vitro. To confirm this result, the inhibitory effect of these plant extracts on the enzymes has been evaluated in vivo. Oral intake of the aqueous extract significantly attenuated starch- and sucrose-induced hyperglycemia in normal rats, and evidently, in STZ-diabetic rats as well. The ethyl acetate extract had no inhibitory activity against the intestinal α-glucosidase enzyme in vivo. The antioxidant and the enzyme inhibitory effects may be related to the presence of naringenin and caffeic acid or their synergistic effect with the other compounds in the extracts.

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Phytochemical profiling, antioxidant, and tyrosinase inhibitory potential of the Acacia cyclops trunk bark: in vitro combined with in silico approach

Kaplan,

Beyaoui,

Jlizi

et al. 2024

Biomedical Chromatography

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The aim of this study was to analyze the phytochemical profile of Acacia cyclops trunk bark ethyl acetate extract using LC–tandem mass spectrometry for the first time, along with evaluating its antioxidant and anti‐tyrosinase properties. Consequently, we determined the total phenolic and flavonoid contents of the extract under investigation and identified and quantified 19 compounds, including phenolic acids and flavonoids. In addition to assessing their antioxidant potential against DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) and ABTS (2,2′‐azino‐bis‐[3‐ethylbenzothiazoline‐6‐sulfonic] acid) assays, in vitro and in silico studies were conducted to evaluate the tyrosinase inhibitory properties of the A. cyclops extract. The ethyl acetate trunk bark extract exhibited a substantial total phenolic content and demonstrated significant antioxidant activity in terms of free radical scavenging, as well as notable tyrosinase inhibitory action (half‐maximal inhibitory concentration [IC50] = 14.08 ± 1.10 μg/mL). The substantial anti‐tyrosinase activity of the examined extract was revealed through molecular docking analysis and druglikeness prediction of the main selected compounds. The findings suggest that A. cyclops extract holds promise as a potential treatment for skin hyperpigmentation disorders.

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Silene vulgaris subsp. macrocarpa leaves and roots from Morocco: assessment of the efficiency of different extraction techniques and solvents on their antioxidant capacity, brine shrimp toxicity and phenolic characterization

Cited by 12 publications

References 31 publications

Brassica incana Ten. (Brassicaceae): Phenolic Constituents, Antioxidant and Cytotoxic Properties of the Leaf and Flowering Top Extracts

Brassica incana Ten. (Brassicaceae): Phenolic Constituents, Antioxidant and Cytotoxic Properties of the Leaf and Flowering Top Extracts

Artemisia absinthium L. Aqueous and Ethyl Acetate Extracts: Antioxidant Effect and Potential Activity In Vitro and In Vivo against Pancreatic α-Amylase and Intestinal α-Glucosidase

Phytochemical profiling, antioxidant, and tyrosinase inhibitory potential of the Acacia cyclops trunk bark: in vitro combined with in silico approach

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