<i>Rhizobium</i> common <i>nod</i> genes are required for biofilm formation

Fujishige, Nancy A.; Lum, Michelle R.; Hoff, Peter L. De; Whitelegge, Julian P.; Faull, Kym F.; Hirsch, Ann M.

doi:10.1111/j.1365-2958.2007.06064.x

Cited by 85 publications

(60 citation statements)

References 42 publications

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“…Recently, studies have shown that the production of LMW EPS II results in biofilm formation and attachment at levels as high as 10-fold greater than those produced by strains of S. meliloti incapable of synthesizing this exopolysaccharide (18,46). Thus, the ability of the bacteria to establish levels of biofilm comparable to wild-type levels was utilized as a secondary indicator of the presence or absence of the low-molecular-weight form.…”

Section: Resultsmentioning

confidence: 99%

Complex Regulation of Symbiotic Functions Is Coordinated by MucR and Quorum Sensing in Sinorhizobium meliloti

Mueller

Gonzalez

2011

J Bacteriol

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In Sinorhizobium meliloti, the production of exopolysaccharides such as succinoglycan and exopolysaccharide II (EPS II) enables the bacterium to invade root nodules on Medicago sativa and establish a nitrogen-fixing symbiosis. While extensive research has focused on succinoglycan, less is known concerning the regulation of EPS II or the mechanism by which it mediates entrance into the host plant. Previously, we reported that the ExpR/Sin quorum-sensing system is required to produce the symbiotically active low-molecular-weight fraction of this exopolysaccharide. Here, we show that this system induces EPS II production by increasing expression of the expG-expC operon, encoding both a transcriptional regulator (ExpG) and a glycosyl transferase (ExpC). ExpG derepresses EPS II production at the transcriptional level from MucR, a RosR homolog, while concurrently elevating expression of expC, resulting in the synthesis of the low-molecular-weight form. While the ExpR/Sin system abolishes the role of MucR on EPS II production, it preserves a multitude of other quorumsensing-independent regulatory functions which promote the establishment of symbiosis. In planktonic S. meliloti, MucR properly coordinates a diverse set of bacterial behaviors by repressing a variety of genes intended for expression during symbiosis and enhancing the bacterial ability to induce root nodule formation. Quorum sensing precisely modulates the functions of MucR to take advantage of both the production of symbiotically active EPS II as well as the proper coordination of bacterial behavior required to promote symbiosis.

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Section: Resultsmentioning

confidence: 99%

Complex Regulation of Symbiotic Functions Is Coordinated by MucR and Quorum Sensing in Sinorhizobium meliloti

Mueller

Gonzalez

2011

J Bacteriol

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“…Moreover, AHL supplementation of rhizobial inoculants did not stimulate strain competitiveness and only slightly increased plant host nodulation. Nevertheless, taking into consideration that (i) numerous traits are controlled by quorum sensing regulation [28,29], (ii) the expression of rhizobial nod genes may be influenced by the metabolic status of the cells [57], and (iii) nod genes as well as quorum sensing signaling may be involved in biofilm formation [58], a connection between the presence of AHL and Nod factor synthesis cannot be excluded. If that were the case, the effect of AHL supplementation of rhizobial inoculants would be similar but weaker than the flavonoid induction and would affect plant growth (but no rhizobial competitiveness) via Nod factor synthesis.…”

Section: Discussionmentioning

confidence: 99%

The effect of biotic and physical factors on the competitive ability of Rhizobium leguminosarum

et al. 2011

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“…CM 98 roots in saline soil (Qurashi & Sabri 2012). Symbiotic genes are important for biofilm-forming rhizobia (Fujishige et al 2008). It seems that biofilm formation is positively connected with the microbe-plant symbiosis.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of endophytic bacteria from a desert plant Lepidium perfoliatum L.

Li¹,

Chen²,

An³

2017

Plant Prot. Sci.

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Li Y., Cheng C., An D. (2017): Characterisation of endophytic bacteria from a desert plant Lepidium perfoliatum L. Plant Protect. Sci., 53: 32-43.Sixty-two endophytic bacteria from the leaves, roots, and stems of healthy Lepidium perfoliatum L. were isolated and characterised. From the results, 89, 87, 90, and 97% isolates could tolerate 12% NaCl, 30% PEG 6000, 50°C and pH 10, respectively. 74% isolates could form a biofilm. Besides, 28 isolates could improve the germination rate of host seeds under different degree of drought stress. These data suggest that the endophyte isolates show considerable resistance to abiotic stress and assist their plant hosts to germinate under drought stress.

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Rhizobium common nod genes are required for biofilm formation

Cited by 85 publications

References 42 publications

Complex Regulation of Symbiotic Functions Is Coordinated by MucR and Quorum Sensing in Sinorhizobium meliloti

Complex Regulation of Symbiotic Functions Is Coordinated by MucR and Quorum Sensing in Sinorhizobium meliloti

The effect of biotic and physical factors on the competitive ability of Rhizobium leguminosarum

Characterisation of endophytic bacteria from a desert plant Lepidium perfoliatum L.

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