Plant Defensin type 1 (<scp>PDF</scp>1): protein promiscuity and expression variation within the Arabidopsis genus shed light on zinc tolerance acquisition in Arabidopsis halleri

Shahzad, Zaigham; Ranwez, Vincent; Fizames, Cécile; Marqués, L. Pons; Martret, Bénédicte Le; Alassimone, Julien; Godé, Cécile; Lacombe, Éric; Castillo, Teddy; Saumitou‐Laprade, Pierre; Berthomieu, Pierre; Gosti, Françoise

doi:10.1111/nph.12396

Cited by 53 publications

(58 citation statements)

References 105 publications

(170 reference statements)

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“…These peptides are small highly stable cysteine-rich antimicrobial peptides, which are present in various multicellular organisms, including fungi, plants and animals. Defensins and defensin-like peptides are recognized as involved in the host's defense against bacteria [88], fungi [57,87] and viruses [19,56,112] and also acting in the plant's tolerance response to abiotic stresses [46,66,92].…”

Section: Introductionmentioning

confidence: 99%

Cysteine-stabilized αβ defensins: From a common fold to antibacterial activity

Dias

Franco

2015

Peptides

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Antimicrobial peptides (AMPs) seem to be promising alternatives to common antibiotics, which are facing increasing bacterial resistance. Among them are the cysteine-stabilized αβ defensins. These peptides are small, with a length ranging from 34 to 54 amino acid residues, cysteine-rich and extremely stable, normally composed of an α-helix and three β-strands stabilized by three or four disulfide bonds and commonly found in several organisms. Moreover, animal and plant CSαβ defensins present different specificities, the first being mainly active against bacteria and the second against fungi. The role of the CSαβ-motif remains unknown, but a common antibacterial mechanism of action, based on the inhibition of the cell-wall formation, has already been observed in some fungal and invertebrate defensins. In this context, the present work aims to group the data about CSαβ defensins, highlighting their evolution, conservation, structural characteristics, antibacterial activity and biotechnological perspectives.

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Section: Introductionmentioning

confidence: 99%

Cysteine-stabilized αβ defensins: From a common fold to antibacterial activity

Dias

Franco

2015

Peptides

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“…Recent characterizations of each PDF1 of the multigenic family showed that, in the Arabidopsis genus, PDF1 proteins are mostly promiscuous in their zinc tolerance and antifungal roles, i.e., the same molecule can convey zinc tolerance to yeast ( Saccharomyces cerevisiae ) while inhibiting in vitro fungal pathogen growth ( Fusarium oxysporum f. sp. melonii ) (Marques et al, 2009; Shahzad et al, 2013). Overall, these datasets indicate that PDF1 s have a pivotal role in the plant response to both biotic (response to pathogens) and abiotic stress (here zinc excess).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary tinkering of the expression of PDF1s suggests their joint effect on zinc tolerance and the response to pathogen attack

et al. 2014

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Multigenic families of Plant Defensin type 1 (PDF1) have been described in several species, including the model plant Arabidopsis thaliana as well as zinc tolerant and hyperaccumulator A. halleri. In A. thaliana, PDF1 transcripts (AtPDF1) accumulate in response to pathogen attack following synergic activation of ethylene/jasmonate pathways. However, in A. halleri, PDF1 transcripts (AhPDF1) are constitutively highly accumulated. Through an evolutionary approach, we investigated the possibility of A. halleri or A. thaliana species specialization in different PDF1s in conveying zinc tolerance and/or the response to pathogen attack via activation of the jasmonate (JA) signaling pathway. The accumulation of each PDF1 from both A. halleri and A. thaliana was thus compared in response to zinc excess and MeJA application. In both species, PDF1 paralogues were barely or not at all responsive to zinc. However, regarding the PDF1 response to JA signaling activation, A. thaliana had a higher number of PDF1s responding to JA signaling activation. Remarkably, in A. thaliana, a slight but significant increase in zinc tolerance was correlated with activation of the JA signaling pathway. In addition, A. halleri was found to be more tolerant to the necrotrophic pathogen Botrytis cinerea than A. thaliana. Since PDF1s are known to be promiscuous antifungal proteins able to convey zinc tolerance, we propose, on the basis of the findings of this study, that high constitutive PDF1 transcript accumulation in A. halleri is a potential way to skip the JA signaling activation step required to increase the PDF1 transcript level in the A. thaliana model species. This could ultimately represent an adaptive evolutionary process that would promote a PDF1 joint effect on both zinc tolerance and the response to pathogens in the A. halleri extremophile species.

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“…The effect of water deficiency on changes in the expression of genes encoding defensins is shown in Glycine max [22,36]. Cold also affects the expression of defensin genes [20,21]. Winter wheat has Tad1 gene, the expression of which is induced specifically under cold stress [21].…”

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confidence: 99%

“…There is both constitutive and inducible expression of genes, encoding plant defensins [11,14]. The main function of defensins is to inhibit fungal disease [8,11,15], but for some peptides there are antibacterial activity [16,17], inhibition of trypsin [18], and participation in the formation of resistance to heavy metals [19], cold stress [20,21], drought [22], salinity [23,24] and in developmental processes [25].…”

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PLANT DEFENSINS: BIOLOGICAL FUNCTION, MECHANISMS OF ACTION AND METHODS OF ANALYSIS (review)

Kulaeva¹,

Kliukova²,

Повыдыш³

et al. 2017

S-h. biol.

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A b s t r a c tOne of the actual problems of modern agriculture is crop loss due to various biotic and abiotic factors. In plants there is a multicomponent protection system, including the formation of protective barriers, activation the reaction of hypersensitivity and synthesis of antimicrobial peptides, which are low molecular weight compounds showing broad spectrum activity against fungi, bacteria and viruses. This group consists of several groups of peptides, including defensins, which are one of the most common classes of antimicrobial peptides and are detectable in all living organisms. Defensins are small (45-54 amino acids), cysteine-rich peptides involved in a different protective responses (B.P. Thomma et al., 2002). Genes which are coding plant defensins are expressed in different organs of plants, where their products are necessary for biotic and abiotic stresses. Thus, these peptides are extremely important in terms of getting crop lines that are resistant to pathogens and abiotic stresses. Defensins are characterized by a strong sequence variability that seems to correlate with a variety of mechanisms of action of these peptides that can induce pathogen's cell death by penetrating into a cell or by being on its surface (T.M. Shafee et al., 2016). Most of the plant defensins are characterized by the antifungal activity. Some defensins have antibacterial activity, which may be combined with activity against fungi. For a small number of plant defensins their participation in the processes of resistance to heavy metals, cold stress, drought, salinity, and in the development process is indicated. Modern approaches of molecular and computational biology allow an effective search for new forms of defensins activity by studying the wild, non-model plant objects. The development of next-generation sequencing methods («Next Generation Sequencing») make possible the intensive study of the transcriptomes of such objects. However, the correct annotation of the sequences of peptides, characterized by small size and high variability, can be done by usage the special programs, such as SPADA (Small Peptide Alignment Discovery Application) (P. Zhou et al., 2013). SPADA makes multiple sequence alignment of all known paralogous genes within a gene family and builds a predictive model for the search of new members of the same family. Prediction of newly identified active defensins and identification of conserved amino acids can also be performed by computational biology methods. An approach based on a multiple sequence alignment and subsequent cluster analysis allows dividing defensins into groups with similar functional activity (N.L. van der Weerden et al., 2013). Thus, the combination of modern methods of molecular and computational biology allows carrying out the study of this group of protective peptides with high efficiency.

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Plant Defensin type 1 (PDF1): protein promiscuity and expression variation within the Arabidopsis genus shed light on zinc tolerance acquisition in Arabidopsis halleri

Cited by 53 publications

References 105 publications

Cysteine-stabilized αβ defensins: From a common fold to antibacterial activity

Cysteine-stabilized αβ defensins: From a common fold to antibacterial activity

Evolutionary tinkering of the expression of PDF1s suggests their joint effect on zinc tolerance and the response to pathogen attack

PLANT DEFENSINS: BIOLOGICAL FUNCTION, MECHANISMS OF ACTION AND METHODS OF ANALYSIS (review)

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