<i>Phytopythium:</i> origin, differences and meaning in modern plant pathology

Tkaczyk, Miłosz

doi:10.2478/ffp-2020-0022

Cited by 2 publications

(2 citation statements)

References 34 publications

(39 reference statements)

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“…The closest relative from oomycetes, Phytopythium paucipapillatum, was isolated in South Africa, and may be a soil inhabitant [45]. The genus Phytopythium includes more than 20 species, most of which are saprophytic [46]. Pedospumella sinomuralis soil colourless Chrysophyta was described in China, 795 m asl [47].…”

Section: Discussionmentioning

confidence: 99%

Amazing Discoveries of Benthic Fauna from the Abyssal Zone of Lake Baikal

Kondratov

Sitnikova

Kaygorodova

et al. 2021

Biology

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Lake Baikal is a natural laboratory for the study of species diversity and evolution, as a unique freshwater ecosystem meeting the all of the main criteria of the World Heritage Convention. However, despite many years of research, the true biodiversity of the lake is clearly insufficiently studied, especially that of deep-water benthic sessile organisms. For the first time, plastic waste was raised from depths of 110 to 190 m of Lake Baikal. The aim of this study was to examine the biological community inhabiting the plastic substrate using morphological and molecular genetic analysis. Fragments of plastic packaging materials were densely populated: bryozoans, leeches and their cocoons, capsules of gastropod eggs, and turbellaria cocoons were found. All the data obtained as a result of an analysis of the nucleotide sequences of the standard bar-coding fragment of the mitochondrial genome turned out to be unique. Our results demonstrate the prospects for conducting comprehensive studies of artificial substrates to determine the true biodiversity of benthos in the abyssal zone of Lake Baikal.

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Section: Discussionmentioning

confidence: 99%

Amazing Discoveries of Benthic Fauna from the Abyssal Zone of Lake Baikal

Kondratov

Sitnikova

Kaygorodova

et al. 2021

Biology

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“…Phytopythium is a genus in the family Pythiaceae of oomycete. At present, there are over 20 species in this genus, with most of them showing strong association with the freshwater environment ( Tkaczyk, 2020 ) and being saprophytes. However, a few Phytopythium species have been observed to cause diseases in plants, such as Phytopythium litorale infecting the Old-World sycamore Platanus orientalis ( Derviş et al, 2020 ); Phytopythium helicoides causing citrus fruit and root rot ( Chen X. R. et al, 2016 ); and Phytopythium vexans (de Bary) ( Lévesque et al, 2008 ; Bala et al, 2010 ) causing root rot in many economically important plants ( Adhikari et al, 2013 ; de Cock et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Development of a Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Phytopythium vexans

Wang¹,

Yongting³

et al. 2021

Front. Microbiol.

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Brown root rot caused by Phytopythium vexans is a new destructive root disease on many plants such as Gingko, Citrus, kiwifruit, and ramie. The establishment of loop-mediated isothermal amplification (LAMP) technology for detecting P. vexans can help monitor and control brown root rot quickly, efficiently, and accurately. LAMP technology is known for its simplicity, sensitivity, and speed; and it does not require any specialized equipment – a water bath or a thermoblock is sufficient for isothermal amplifications. LAMP products can be visualized by using hydroxy naphthol blue (HNB) dye or agarose gel electrophoresis. In this study, by searching and comparing the internal transcribed spacer (ITS) sequences of P. vexans and the related species in oomycete genera Pythium, Phytopythium, and Phytophthora, we designed specific primers targeting the ITS gene region of P. vexans. Using HNB dye, we established a LAMP technique for rapid detection of P. vexans by visible color change. In addition, we optimized the protocol to enhance both sensitivity and specificity for P. vexans detection. Under the optimized condition, our protocol based on LAMP technology could detect as low as 24 copies of the P. vexans genomic DNA, which is ∼100 times more sensitive than conventional PCR. This method can successfully detect P. vexans using cell suspensions from P. vexans – infected ramie root tissues.

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Phytopythium: origin, differences and meaning in modern plant pathology

Cited by 2 publications

References 34 publications

Amazing Discoveries of Benthic Fauna from the Abyssal Zone of Lake Baikal

Amazing Discoveries of Benthic Fauna from the Abyssal Zone of Lake Baikal

Development of a Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Phytopythium vexans

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