Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid

Abstract

Whole cells of the basidiomycete fungus Phanerochaete chrysosporium (ATCC 20696) were applied to induce the biomodification of lignin in an in vivo system. Our results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin through a series of redox reactions, leading not only to the degradation of lignin but also to its polymerization. The reducing agents ascorbic acid and α-tocopherol were used to stabilize the free radicals generated from the ligninolytic process. The application of P. chrysosporium in combination with reducing agents produced aromatic compounds and succinic acid as well as degraded lignin polymers. P. chrysosporium selectively catalyzed the conversion of lignin to succinic acid, which has an economic value. A transcriptomic analysis of P. chrysosporium suggested that the bond cleavage of synthetic lignin was caused by numerous enzymes, including extracellular enzymes such as lignin peroxidase and manganese peroxidase, and that the aromatic compounds released were metabolized in both the short-cut and classical tricarboxylic acid cycles of P. chrysosporium. In conclusion, P. chrysosporium is suitable as a biocatalyst for lignin degradation to produce a value-added product.

Whole-Genome De Novo Sequencing of the Lignin-Degrading Wood Rot Fungus Phanerochaete chrysosporium (ATCC 20696)

“…These results provide insight into the complex extra- and intracellular enzyme systems of P. chrysosporium (ATCC 20696). Based on this complex enzyme pool, P. chrysosporium has an excellent catabolic ability to convert aromatics to acid compounds, which supports the biodegradation mechanism of lignin by P. chrysosporium suggested in our previous study ( 8 ).…”

“…Our previous study found that P. chrysosporium (ATCC 20696) degraded monolignols and synthetic lignin to succinic acid ( 8 ). This finding suggested that P. chrysosporium (ATCC 20696) catalyzed not only reactions involving side chains of lignin compounds, but also ring cleavage, indicating that this fungus uses a unique catabolic system in lignin modification.…”

Intergrated Metagenomics and Metabolomics Analysis Discovers Nematicidal Microbes, Enzymes and Metabolites From the Plant Rhizosphere Microbiota

“…Phanerochaete chrysosporium is a decomposer in carbon cycle (such as lignin degradation) [40]. These results suggested that the composition of microbial networks had varied considerably in the RNI soils when compared with the NS soils.…”

Intergrated metagenomics and metabolomics analysis discovers nematicidal microbes, enzymes and metabolites from the plant rhizosphere microbiota

“…Among them, Rhizophagus irregularis, an arbuscular mycorrhizal fungi, can improve phosphorus uptake and water use efficiency in plants [92]. Phanerochaete chrysosporium is a decomposer in carbon cycle [93]. Macrophomina phaseolina produced macrolide [94].…”