OsRuvB transgene induces salt tolerance in pigeon pea

Abstract

Salinity of cultivable land is a growing global concern that has been affecting the yields of major crops worldwide such as pigeon pea. In the current study, transgenic pigeon pea plants were developed using an in-planta Agrobacterium-mediated genetic transformation method wherein OsRuvB, a rice DNA helicase gene, was incorporated to induce salt tolerance in pigeon pea plants. Transformation efficiency of 35.7% was achieved with stable insertion of OsRuvB in transgenic lines. When subjected to salinity stress induced by 75 mM NaCl increase in chlorophyll content, relative water content, peroxidase and catalase activity in transgenic lines was observed over the wild type plants. Membrane injury index and lipid peroxidation were significantly reduced in transgenic lines. Proline and Total Soluble Sugar content were enhanced in both transgenic plants and wild type strains. It was inferred that transgenic lines were tolerant to salinity stress and tolerance may be imparted through an alternative unknown pathway. ARTICLE HISTORY

A Genotype-Independent, Simple, Effective and Efficient in Planta Agrobacterium-Mediated Genetic Transformation Protocol

“…The presence of a single band observed by Southern hybridization analysis revealed that all the transgenic lines developed in various crops reported here ( Table 2 ) carried a single copy of the transgene ( Figure 2 ), except transgenic pigeon pea lines separately carrying the OsRuv B gene and the OsLecRLK gene, where one of line each was observed to carry two copies (last lane in Figure 2 B; third lane in Figure 2 G) as two bands in each of these lines were detected. Similar results were observed by real time-PCR analysis [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ].…”

“…For developing other transgenic lines mentioned in Table 2 [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ], the seeds were directly transferred to potted soil after co-cultivation, omitting the step of germinating in the presence of cefotaxime. For wheat and rice transformation, 200 µM acetosyringone was added to the bacterial suspension during co-cultivation.…”

Pea transformation: History, current status and challenges

“…A number of traits have been improved by transformation in pigeon peas also, e.g., insect resistance (Krishna et al 2011;Kaur et al 2016;Ghosh et al 2017), increased salt tolerance (Singh et al 2020) and seed nutritional quality (Thu et al 2007). Insect resistance (Solleti et al 2008;Grazziotin et al 2020) and virus resistance (Cruz & Aragão 2013;Kumar et al 2017) were induced by transformation methods in the cowpea also.…”

Fab Advances in Fabaceae for Abiotic Stress Resilience: From ‘Omics’ to Artificial Intelligence

“…Kwapata et al [ 157 ] created a drought-tolerant common bean crop using Hordeum vulgare ’s late embryogenesis abundant (LEA) protein HVA1 . Likewise, Singh et al utilized the rice DNA helicase ( OsRuvB ) gene to confer salinity tolerance in pigeonpea [ 158 ]. Similarly, Hanafy et al heterologously expressed the potato gene PR10a in faba bean to enhance its salinity and drought tolerance [ 159 ].…”