Lactobacillus rhamnosus Granules Dose-Dependently Balance Intestinal Microbiome Disorders and Ameliorate Chronic Alcohol-Induced Liver Injury

Abstract

As the functions of Lactobacilli become better understood, there are increasing numbers of applications for Lactobacillus products. Previously, we have demonstrated that Lactobacillus rhamnosus GG (LGG) can prevent alcoholic liver injury. LGG granules were produced by fluid bed granulation with a media composed of starch, skimmed milk powder, whey powder, microcrystalline cellulose and maltose, and LGG fermented liquid that comprised 30-50% of the total weight. We found LGG granules dose-dependently protected against chronic alcoholic liver disease. When alcohol was consumed for 8 weeks with LGG treatment during the last 2 weeks, we demonstrated that the dose dependence of LGG granules can improve alcoholinduced liver injury through decreasing the levels of lipopolysaccharide and tumor necrosis factor-a in serum and prevent liver steatosis by suppressing triglyceride, free fatty acid, and malondialdehyde production in liver. Alcohol feeding caused a decline in the number of both Lactobacillus and Bifidobacterium, with a proportional increase in the number of Clostridium perfringens in ileum, and expansion of the Gram-negative bacteria Proteobacteria, Campylobacterales, and Helicobacter in cecum. However, LGG granule treatment restored the content of these microorganisms. In conclusion, LGG granule supplementation can improve the intestinal microbiota, reduce the number of gram-negative bacteria, and ameliorate alcoholic liver injury.

Protective effect of Lactobacillus salivarius Li01 on thioacetamide‐induced acute liver injury and hyperammonaemia

“…However, the L. salivarius Li01 intervention changed the structure of the gut microbiota and increased the relative abundance of Lactobacillus. The TAA injection favoured Proteobacteria and Helicobacter, which include many pathogens, have been related to liver injury in a previous study (Gu et al, 2020), and were positively correlated with systematic inflammation in our study. However, the L. salivarius Li01 pretreatment reduced these bacteria.…”

Dendropanax morbifera Leaf Extracts Improved Alcohol Liver Injury in Association with Changes in the Gut Microbiota of Rats

“…In this study, we observed that alcohol consumption increased Prevotella and Parasutterella and decreased Clostridium , Turcibacter and Romboutsia , which is in line with the previous reports [ 46 , 47 , 48 , 49 ]. Moreover, alcohol consumption also increased the abundance of some of the gram-negative bacteria (i.e, Porphyromonadaceae and Alloprevotella ).…”

Alcoholic fatty liver disease inhibited the co-expression of Fmo5 and PPARα to activate the NF-κB signaling pathway, thereby reducing liver injury via inducing gut microbiota disturbance

“…The accumulation of harmful substances such as alcohol leads to liver injury and promotes the secretion of bile acid into the intestinal tract by the liver, thereby regulating the activity of various metabolic reactions in the intestinal tract [2,4,5,13]. The change in the type and number of intestinal flora is an important factor leading to abnormal liver function [20]. Liver disease can also lead to overgrowth and imbalance of intestinal flora [5,17].…”