Lactobacillus pentosus CECT 4023 T co‐utilizes glucose and xylose to produce lactic acid from wheat straw hydrolysate: Anaerobiosis as a key factor

Abstract

Lactic acid is a versatile chemical that can be produced via fermentation of lignocellulosic materials. The heterolactic strain Lactobacillus pentosus CECT 4023 T, that can consume glucose and xylose, was studied to produce lactic acid from steam exploded wheat straw prehydrolysate. The effect of temperature and pH on bacterial growth was analyzed. Besides, the effect of oxygen on lactic acid production was tested and fermentation yields were compared in different scenarios. This strain showed very high tolerance to the inhibitors contained in the wheat straw prehydrolysate. The highest lactic acid yields based on present sugar, around 0.80 g g−1, were obtained from glucose in presence of 25%, 50%, and 75% v v−1 of prehydrolysate in strict anaerobiosis. Lactic fermentation of wheat straw hydrolysate obtained after enzymatic hydrolysis of the prehydrolysate yielded 0.39 g of lactic acid per gram of released sugars, which demonstrated the high potential of L. pentosus to produce lactic acid from hemicellulosic hydrolysates. Results presented herein not only corroborated the ability of L. pentosus to grow using mixtures of sugars, but also demonstrated the suitability of this strain to be applied as an efficient lactic acid producer in a lignocellulosic biorefinery approach. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2739, 2019

Parametric optimization and kinetic study of l‐lactic acid production by homologous batch fermentation of Lactobacillus pentosus cells

“…Cubas‐Cano et al. observed the highest yield of 0.80 g/g substrate in the fermentation of wheat straw hydrolysate by L. pentosus CECT 4023 T cells [53]. Zhang and Vadlani studied the cofermentation of L. brevis and L. plantarum for LA production and obtained the highest LA yield of 0.8 g/g substrate [54].…”

Do regrowth age and microbial inoculant usage affect the microbial diversity and fermentation characteristics of forage peanut silage?

“…Lactiplantibacillus pentosus is a facultative heterolactic bacterium that can ferment both hexoses and pentoses as substrate sources. This LAB species uses the Embden–Meyerhof–Parnas pathway to ferment glucose and the phosphoketolase pathway to ferment xylose, ribose, deoxyribose, and other pentoses (Cubas‐Cano et al, 2018; Pahlow et al, 2003). Depending on substrate availability, this strain can produce lactic and acetic acid through the phosphoketolase pathway, resulting in heterolactic fermentation and reduced DM recovery compared to homolactic fermentation (Carvalho et al, 2021; McDonald et al, 1991).…”

Antihypertensive effects of abalone viscera fermented with Lactiplantibacillus pentosus SN001 via angiotensin-converting enzyme inhibition

“…The blood glucose levels in the fermented group were the lowest and were approximately 20 mg/dL lower than those in the control group, although the difference was not significant. LAB consume glucose during metabolism ( Cubas-Cano, González-Fernández, Ballesteros, & Tomás-Pejó, 2018 ). In addition, administration of a closely related species, L. plantarum , decreases the amount of glucose in the blood ( da Costa et al, 2019 ).…”