<i>In Vivo</i>Uptake of Chitosan Microparticles by Murine Peyer's Patches: Visualization Studies using Confocal Laser Scanning Microscopy and Immunohistochemistry

Lubben, I.M. van der; Konings, Frank; Borchard, Gerrit; Verhoef, J.; Junginger, Hans E.

doi:10.3109/10611860108995631

Cited by 101 publications

(33 citation statements)

References 13 publications

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“…Table 1 provides examples of the main non-viral methods of gene delivery. Deacetylation of chitin can be performed by boiling chitin from crab or shrimp shells in sodium hydroxide after decolourisation with potassium permanganate (Van Der Lubben et al, 2001). Chitosan is a co-polymer of glucosamine and N-acetyl-D-glucosamine.…”

Section: Non-viral Vectors For Gene Deliverymentioning

confidence: 99%

Chitosan and Its Modifications: Are They Possible Vehicles for Gene Therapy?

Kedjarune‐Leggat¹,

Leggat²

2011

Non-Viral Gene Therapy

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Section: Non-viral Vectors For Gene Deliverymentioning

confidence: 99%

Chitosan and Its Modifications: Are They Possible Vehicles for Gene Therapy?

Kedjarune‐Leggat¹,

Leggat²

2011

Non-Viral Gene Therapy

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“…Mice were sacrificed after 4 h of dose administration by cervical dislocation, and intestinal Peyer's patch were removed using standard procedure. The samples were analyzed under confocal microscope (Nikon, Japan) ( Van der Lubben et al, 2001b).…”

Section: Peyer's Patch Uptake Studymentioning

confidence: 99%

Development and characterization of guar gum nanoparticles for oral immunization against tuberculosis

et al. 2014

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The main aim of this study was to develop an effective carrier system containing Ag85A-loaded guar gum nanoparticles for oral vaccination against tuberculosis. Nanoparticles were prepared by Nanoprecipitation method. The developed particles with mean diameter 895.5 ± 14.73 nm and high antigen entrapment seem to be optimum for oral vaccine delivery. The acid protection assay, Peyer's patch uptake study and in-vitro antigen study confirmed that the developed formulations can protect the antigen from harsh gastric environment and can safely deliver the antigen to the intestinal region. In vivo studies data indicated that the developed nanocarriers can induce a strong mucosal as well as systemic immune response. Therefore, the experimental evidence suggests that guar-gum nanoparticle findings indicated that the guar gum nanoparticles can be utilized for safe and effective vaccine delivery via oral route.

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“…Several studies have shown that the uptake by M-cells was significantly enhanced and degradation of protein and peptide vaccines in the gastrointestinal track was prevented after the incorporation of vaccine with CMs. 59,[68][69][70][71] Due to its nontoxicity and potent antigen binding properties, chitosan has been considered a promising tool for oral vaccination.…”

Section: Oral Deliverymentioning

confidence: 99%

Design and application of chitosan microspheres as oral and nasal vaccine carriers: an updated review

Islam¹,

Firdous²,

Choi³

et al. 2012

IJN

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Chitosan, a natural biodegradable polymer, is of great interest in biomedical research due to its excellent properties including bioavailability, nontoxicity, high charge density, and mucoadhesivity, which creates immense potential for various pharmaceutical applications. It has gelling properties when it interacts with counterions such as sulfates or polyphosphates and when it crosslinks with glutaraldehyde. This characteristic facilitates its usefulness in the coating or entrapment of biochemicals, drugs, antigenic molecules as a vaccine candidate, and microorganisms. Therefore, chitosan together with the advance of nanotechnology can be effectively applied as a carrier system for vaccine delivery. In fact, chitosan microspheres have been studied as a promising carrier system for mucosal vaccination, especially via the oral and nasal route to induce enhanced immune responses. Moreover, the thiolated form of chitosan is of considerable interest due to its improved mucoadhesivity, permeability, stability, and controlled/extended release profile. This review describes the various methods used to design and synthesize chitosan microspheres and recent updates on their potential applications for oral and nasal delivery of vaccines. The potential use of thiolated chitosan microspheres as next-generation mucosal vaccine carriers is also discussed.

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In VivoUptake of Chitosan Microparticles by Murine Peyer's Patches: Visualization Studies using Confocal Laser Scanning Microscopy and Immunohistochemistry

Cited by 101 publications

References 13 publications

Chitosan and Its Modifications: Are They Possible Vehicles for Gene Therapy?

Chitosan and Its Modifications: Are They Possible Vehicles for Gene Therapy?

Development and characterization of guar gum nanoparticles for oral immunization against tuberculosis

Design and application of chitosan microspheres as oral and nasal vaccine carriers: an updated review

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