2013
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Abstract: In various stem cell therapy approaches poor cell survival has been recognized as an important factor limiting therapeutic efficacy. Therefore noninvasive monitoring of cell fate is warranted for developing clinically effective stem cell therapy. In this study we investigated the use of voxel-based R₂ mapping as a tool to monitor the fate of iron oxide-labeled cells in the myocardium. Mesenchymal stem cells were transduced with the luciferase gene, labeled with ferumoxide particles and injected in the myocardi… Show more

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“…In our previous studies, we proved that USPIO labeling had no effects on cell structure, viability, proliferation by electron microscopy, trypan blue test and MTS assay. [3] The question remains, as illustrated by our previous studies and others [13,16], that USPIO particles could be diluted by cell division or degraded through an iron metabolic pathway, thus leading to false-negative results. In addition, USPIO particles could also remain in the extracellular matrix after cell death and lysis, or they could be engulfed by macrophages and remain in the injected region, thus leading to false-positive results.…”
Section: Discussionmentioning
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rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…In our previous studies, we proved that USPIO labeling had no effects on cell structure, viability, proliferation by electron microscopy, trypan blue test and MTS assay. [3] The question remains, as illustrated by our previous studies and others [13,16], that USPIO particles could be diluted by cell division or degraded through an iron metabolic pathway, thus leading to false-negative results. In addition, USPIO particles could also remain in the extracellular matrix after cell death and lysis, or they could be engulfed by macrophages and remain in the injected region, thus leading to false-positive results.…”
Section: Discussionmentioning
“…Although exogenous stem cell labelling with superparamagnetic iron oxide nanoparticles prior to stem cell transplantation is currently the most employed cell labelling method in both preclinical and clinical trials 14 15 16 17 18 19 20 , monitoring cell death following transplantation is still a challenge 21 22 23 . Consequently, this is currently an area of active research 24 25 26 27 28 29 30 31 32 33 34 35 36 37 .…”
mentioning
“…Using this strategy, MRI contrast agents-labeled stem cells can be monitored for their movement, localization/migration, survival/proliferation, and differentiation. In addition to a wide range of applications in cell-based therapies, such as ischemic [73], immune [74], and neurodegeneration [75] diseases, stem cells have demonstrated outstanding promise in tissue engineering and regenerative medicine, for example, in the regeneration of injured heart [76], cartilage [77], and bone tissue [78]. For these purposes, IO NPs surface modified with D-mannose [79], poly(L-lysine) [80], poly(N,N-dimethylacrylamide) [81], and oligoperoxide have been prepared and investigated as probes for stem cell labeling [82].…”
Section: Cell Labelingmentioning