In vivocytogenetic studies on rat’s bone-marrow cells of structurally related Schiff base complexes

Abstract

The in vivo interactions of structurally-related Ni(II) and Fe(III) Schiff base complexes based on N-(8-quinolyl)salicylaldimine (HL(1)) and N-(8-quinolyl)napthaldimine (HL(2)) ligands with DNA molecules in the bone-marrow cells of rats were demonstrated using chromosomal aberrations (CAs) assay. The complexes differ by one aromatic group on the aldehyde site of the Schiff base (basicity or lipophilicity), or by the type of the central metal ions (Ni(II) or Fe(III)). Animals were injected intraperitoneally (i.p) with different concentrations of each drug, and CAs were examined in bone-marrow cells, 15 hours later. A significant increase in the frequency of CAs was induced upon treatment with 15 mg / kg weight of L(1) complexes (P < 0.001), and not with L(2) complexes (P > 0.05). Also, the magnitude of aberrations induced by L(1)-Ni(II) was higher than that induced by L(1)-Fe(III) (P < 0.01). The binding data, estimated using UV-Visible absorption technique, showed that the metal binding of HL(1) was much greater than that of HL(2) and that the affinity of HL(1) towards Ni(II) is higher than that for Fe(III) ions. Thus, the trends in the presented in vivo results signify the important role of complex stability in predicting the clastogenicity of metal-ion-chelating Schiff base drugs.

Assessment of DNA damage using chromosomal aberrations assay in lymphocytes of waterpipe smokers

“…The evaluator was blind to the treatment. CAs were divided into gaps (including both chromatid gaps and chromosome gaps), breaks (including both chromatid breaks and chromosome breaks) and exchanges [25]. …”

Genotoxicity assessment in iron deficiency anemia patients using sister chromatid exchanges and chromosomal aberrations assays

“…In the last 2 h of incubation, Colcemid (0.1 g/mL) was added to arrest the cells in metaphase. Cultured cells were harvested and fixed as previously described [20,21]. The cellular suspension was then dropped on prechilled microscope slides to obtain metaphase spreads.…”

Synthesis, characterization and biological activity of some unsymmetrical Schiff base transition metal complexes

“…Schiff base transition metal complexes (Esmadi et al, 2013) have been prepared and characterized. Moreover, the biological activity of some of the prepared complexes had been studied earlier Saleh et al, 2011).…”