<i>In vitro</i>pharmacological screening of macrofungi extracts from the Brazilian northeastern region

Silva, Fabrício Souza; Sá, Matheus Santos de; Costa, José Fernando Oliveira; Pinto, Fernanda Pinheiro Pizzelli; Lima, Milena S.; Lucchese, Angélica Maria; Góes-Neto, Aristóteles; Zalis, Mariano; Ribeiro-dos-Santos, Ricardo; Soares, Milena Botelho Pereira

doi:10.1080/13880200902755226

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…IC 50 values range from 0.23-3.30 μM (Jiang et al, 2009;Jiang et al, 2011). Silva et al, (2009) first reported antitumor activity of methanolic extract (ME) of the Hexagonia papyraceae against K562 (Human chronic myeloid leukemia cell) and Daudi (human Burkitt's lymphoma cell). ME inhibited more than 60% of the proliferation of both K562 and Daudi cells.…”

Section: Discussionmentioning

confidence: 99%

Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines

Ghosh¹,

Sanyal²,

Bera³

2020

Asian Pac J Cancer Prev

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Objective: In this study, we aimed to harness some solvent extracts of one wild mushroom Hexagonia glabra and test their anti-cancer activity against cervical human cell lines, namelyHeLa, SiHa, and CaSki. Methods: It includes cell morphological study by microscope, nuclear morphology by DAPI staining under fluorescence microscopy, apoptosis assay by fluorescence technique, anti-proliferation by MTT assay and expression of apoptotic and anti-apoptotic genes by Western blotting and cell cycle analysis was done. Results: The selected cervical cancer cells were treated separately with 150 µg/mL of three extracts, namely of ethanolic (EE), ethyl acetate (EAE), and water extract (WE) and exhibited features like round, shrink and dead. All extracts caused apoptosis in cell lines and EE had the highest effect in this regard. The percentages of apoptotic cells in HeLa, SiHa and CaSki, at the same concentration of EE were 79.23, 75.42, and 76.36% respectively. Cytotoxicity assay showed that all three extracts (50 – 250 μg/mL) were potent for inhibition of cell growth of three cell lines and again EE had the highest effect. The percentages of cell growth inhibition in HeLa, SiHa, and CaSki cells treated with EE at 24 h at 50 µg/mL were 45.79±4.11, 41.66±4.03, and 36.72±2.67, while they were 74.23±7.45, 62.31±5.97, and 54.23±5.04 at 150 µg/mL concentration. At 250 µg/mL concentration, the percentages of cell growth inhibition were 94.25 ±8.11, 90.02 ±8.67, and 85.43±6.21, respectively. The expression of apoptotic gene ( Caspase 3, 9 ) and tumor guard gene ( p53 ), as their proteins in Western blotting increased . However, anti-apoptotic BcL2 gene of all cell lines was decreased following treatment with extracts. In addition, the cell cycle analysis (CaSki cell) showed that treatment (EE) arrested at G2/M check point cell cycle. Conclusion: All extracts of this mushroom were active in arresting growth of three cell lines and EE had the highest effect, indicating that this mushroom can be a valuable source of anticancer agents.

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Section: Discussionmentioning

confidence: 99%

Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines

Ghosh¹,

Sanyal²,

Bera³

2020

Asian Pac J Cancer Prev

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“…and Phellinus spp., D. caperata and E. scabrosa to inhibit bacterial growth was reported in literature (Smânia et al 1995, 1998, Gogavekar et al 2014, Silva et al 2009, Silva & Jorge 2011, Kalyoncu et al 2010, Wisbeck & Robert 2002. However, the samples used in the current study showed weak or no inhibitory activity against the tested bacteria (Table 1, 4).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of Ethyl Acetate Extract of Agaricomycetes collected in Northeast Brazil

Ferreira-Silva¹

2017

CREAM

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Agaricomycetes produce bioactive substances with antibiotic, antiallergic, antiinflammatory, antioxidant, cytotoxic, antiatherogenic, hepatoprotective, hypoglycemic antiimmunoprotective properties. However, there have been few studies on material collected in Brazil. Thus, the current study aimed to improve the knowledge concerning the antibacterial potential of Agaricomycetes in the country by researching material collected in Northeast Brazil. Twenty one cultures deposited in Micoteca URM of UFPE and nine extracts from fresh basidiomata were tested. Extracts of the basidiomata and the cultures were obtained with ethyl acetate, which were solubilized at a concentration (1:10 v/v). The preliminary tests with the 30 samples (21 cultures and nine extracts of fresh basidiomata) were performed against eleven strains of Staphylococcus aureus, seven of which were resistant to oxacillin (ORSA), using block agar. Thirteen samples showed antibacterial activity, with inhibition between 9-24 mm and minimum inhibitory concentration (MIC) from 0.18-147 µg/mL, Fomitopsis cupreorosea (URM 6830), Ganoderma multiplicatum (URM 6975), G. parvulum (URM 2948), G. orbiforme (URM87741), Grammothele lineata (URM6827), Rigidoporus lineatus (URM 6828), R. microporus (URM 6878), Stereum ostrea (URM 6973, URM 87848) being the most significant.

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Species and functional diversity of macrofungi from protected areas in mountain forest ecosystems of Southern Luzon, Philippines

et al. 2021

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In vitropharmacological screening of macrofungi extracts from the Brazilian northeastern region

Cited by 4 publications

References 23 publications

Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines

Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines

Antibacterial activity of Ethyl Acetate Extract of Agaricomycetes collected in Northeast Brazil

Species and functional diversity of macrofungi from protected areas in mountain forest ecosystems of Southern Luzon, Philippines

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