<i>In vitro</i> model to study the biomaterial-dependent reaction of macrophages in an inflammatory environment

Grotenhuis, Nienke; Toom, H. F. E. vd; Kops, Nicole; Bayon, Yves; Deerenberg, Eva B.; Mulder, Irene M.; Osch, Gerjo J. V. M. van; Lange, Johan F.; Bastiaansen-Jenniskens, Y.M.

doi:10.1002/bjs.9523

Cited by 36 publications

(23 citation statements)

References 27 publications

(51 reference statements)

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“…The use of biomaterials has become common in regenerative medicine. The reaction of primary human macrophages to biomaterials has been shown in vitro to be biomaterial specific, even when an inflammatory situation is simulated [18,19]. However, the person-dependent foreign body response has not been taken into account in these models.…”

Section: Discussionmentioning

confidence: 99%

“…Previous in vitro models have shown that culturing macrophages isolated from healthy donors on different biomaterials leads to a biomaterial-specific reaction [18] and that even in a contaminated in vitro model, surgical biomaterials still elicit differential reactions in macrophages [19]. These in vitro models did not take into account patient specific characteristics, such as age, smoking, diabetes or obesity.…”

Section: Introductionmentioning

confidence: 99%

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Monocyte subsets in blood correlate with obesity related response of macrophages to biomaterials in vitro

et al. 2016

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Macrophages play a key role in the foreign body response. In this study it was investigated whether obesity affects the acute response of macrophages to biomaterials in vitro and whether this response is associated with biomarkers in blood. CD14 monocytes were isolated from blood from obese and age and gender matched lean persons. Monocyte subsets were determined based on CD14 and CD16 on their surface. C-reactive protein (CRP) was measured in peripheral blood. The response of monocyte-derived macrophages to polypropylene (PP), polylactic acid (PLA), polyethylene terephthalate (PET) monofilament, and PET-multifilament (mPET) in culture was based on cytokine production. More IL-6 (for PET), less CCL18 (all materials) and IL-1ra (for PLA) was produced by macrophages from obese patients than lean subjects. Body mass index, serum CRP and to a lesser extend percentages of monocyte subtypes correlated with IL-6, TNFα, CCL18, and IL-1ra production. Taken together, monocyte-derived macrophages of obese patients respond more pro-inflammatory and less anti-inflammatory to biomaterials than macrophages from lean subjects, depending on the material. These results are a step towards personalized medicine for the development of a model or even a blood test to decide which biomaterial might be suitable for each patient.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Monocyte subsets in blood correlate with obesity related response of macrophages to biomaterials in vitro

et al. 2016

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“…e Re-combined culture: different cell types either from primary dissociations or cell lines are combined in known a specified ratio and cultured together. [116].…”

Section: Monocyte/macrophage Culturesmentioning

confidence: 95%

“…Minimal immune cell adhesion to microdevice surfaces leads to decreased macrophages being available to fuse into FBGCs. Regardless of the origin of the immune cells being tested (cell line or primary source), the surface chemistry of the material determines the ability of leukocytes, monocytes and macrophages to attach and spread on it [116]. Immune cell attachment and reactions to a surface are modified by the ability of proteins to first adhere the surface [67].…”

Section: Monocyte/macrophage Culturesmentioning

confidence: 99%

A critical review of cell culture strategies for modelling intracortical brain implant material reactions

et al. 2016

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“…Moreover, the inflammatory environment, simulating bacterial infection, increased proinflammatory cytokine production by macrophages cultured with PET. In contrast, macrophages cultured with polypropylene responded with CCL18 production and demonstrated a low pro/anti-inflammatory cytokine ratio [51]. These studies suggested that choice of biomaterial may be critical when implant-associated infection is possible.…”

Section: Macrophage Responses To Implantsmentioning

confidence: 97%

Macrophage responses to implants: prospects for personalized medicine

Kzhyshkowska

Gudima

Riabov

et al. 2015

Journal of Leukocyte Biology

173

142

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Implants, transplants, and implantable biomedical devices are mainstream solutions for a wide variety of human pathologies. One of the persistent problems around nondegradable metallic and polymeric implants is failure of macrophages to resolve the inflammation and their tendency to stay in a state, named "frustrated phagocytosis." During the initial phase, proinflammatory macrophages induce acute reactions to trauma and foreign materials, whereas tolerogenic antiinflammatory macrophages control resolution of inflammation and induce the subsequent healing stage. However, implanted materials can induce a mixed pro/ anti-inflammatory phenotype, supporting chronic inflammatory reactions accompanied by microbial contamination and resulting in implant failure. Several materials based on natural polymers for improved interaction with host tissue or surfaces that release antiinflammatory drugs/bioactive agents have been developed for implant coating to reduce implant rejection. However, no definitive, long-term solution to avoid adverse immune responses to the implanted materials is available to date. The prevention of implant-associated infections or chronic inflammation by manipulating the macrophage phenotype is a promising strategy to improve implant acceptance. The immunomodulatory properties of currently available implant coatings need to be improved to develop personalized therapeutic solutions. Human primary macrophages exposed to the implantable materials ex vivo can be used to predict the individual's reactions and allow selection of an optimal coating composition. Our review describes current understanding of the mechanisms of macrophage interactions with implantable materials and outlines the prospects for use of human primary macrophages for diagnostic and therapeutic approaches to personalized implant therapy. J. Leukoc. Biol. 98: 953-962;

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In vitro model to study the biomaterial-dependent reaction of macrophages in an inflammatory environment

Abstract: Macrophages in an inflammatory environment in vitro still react in a biomaterial-dependent manner. This model can help to select biomaterials that are tolerated best in a surgical environment at risk of contamination.

Cited by 36 publications

References 27 publications

Monocyte subsets in blood correlate with obesity related response of macrophages to biomaterials in vitro

Monocyte subsets in blood correlate with obesity related response of macrophages to biomaterials in vitro

A critical review of cell culture strategies for modelling intracortical brain implant material reactions

Macrophage responses to implants: prospects for personalized medicine

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