<i>In vitro</i> interaction between surface‐treated Ti‐6Al‐4V titanium alloy and human peripheral blood mononuclear cells

Martinesi, Maria; Bruni, Sara; Stio, Maria; Treves, Cristina; Borgioli, Francesca

doi:10.1002/jbm.a.30366

Cited by 20 publications

(17 citation statements)

References 46 publications

(36 reference statements)

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“…It was suggested that Ni 2+ and Co 2+ -induced CAM expression could be a result from metal ion attachment to proteins presented to the cultured ECs [20], due to the involvement of oxygen radicals [18,82], or due to the inhibition of the biological activity of nitric oxide [82]. More recent studies focused on the culture of ECs with bulk permanent implant materials and reported: no significant CAM expression after culture with NiTi, CoCrNi, or NiCr [21]; significant ICAM-1 and VCAM-1 expression induced by the ion release from Ti-6Al-4V alloy [22]; and significant E-selectin expression induced by the ion release from NiTi wires [23]. …”

Section: Discussionmentioning

confidence: 99%

Cytocompatibility and early inflammatory response of human endothelial cells in direct culture with Mg-Zn-Sr alloys

et al. 2017

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Crystalline Mg-Zinc (Zn)-Strontium (Sr) ternary alloys consist of elements naturally present in the human body and provide attractive mechanical and biodegradable properties for a variety of biomedical applications. The first objective of this study was to investigate the degradation and cytocompatibility of four Mg-4Zn-xSr alloys (x = 0.15, 0.5, 1.0, 1.5 wt%; designated as ZSr41A, B, C, and D respectively) in the direct culture with human umbilical vein endothelial cells (HUVEC) in vitro. The second objective was to investigate, for the first time, the early-stage inflammatory response in cultured HUVECs as indicated by the induction of vascular cellular adhesion molecule-1 (VCAM-1). The results showed that the 24-h in vitro degradation of the ZSr41 alloys containing a β-phase with a Zn/Sr at% ratio ~1.5 was significantly faster than the ZSr41 alloys with Zn/Sr at% ~1. Additionally, the adhesion density of HUVECs in the direct culture but not in direct contact with the ZSr41 alloys for up to 24 h was not adversely affected by the degradation of the alloys. Importantly, neither culture media supplemented with up to 27.6 mM Mg2+ ions nor media intentionally adjusted up to alkaline pH 9 induced any detectable adverse effects on HUVEC responses. In contrast, the significantly higher, yet non-cytotoxic, Zn2+ ion concentration from the degradation of ZSr41D alloy was likely the cause for the initially higher VCAM-1 expression on cultured HUVECs. Lastly, analysis of the HUVEC-ZSr41 interface showed near-complete absence of cell adhesion directly on the sample surface, most likely caused by either a high local alkalinity, change in surface topography, and/or surface composition. The direct culture method used in this study was proposed as a valuable tool for studying the design aspects of Zn-containing Mg-based biomaterials in vitro, in order to engineer solutions to address current shortcomings of Mg alloys for vascular device applications.

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Section: Discussionmentioning

confidence: 99%

Cytocompatibility and early inflammatory response of human endothelial cells in direct culture with Mg-Zn-Sr alloys

et al. 2017

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“…Petri dishes (℘ 60 mm) with HUVEC cocultured with PBMC stimulated with 1.5 μg/mL PHA were used as controls. At the end of the coculture incubation time, PBMC were removed by vigorous washing with cold PBS and the detachment of monocytes was controlled by phase‐contrast microscopy, as previously reported 33. Then HUVEC were collected for the determination of ICAM‐1, VCAM‐1, and E‐selectin by Western blot analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The optical density was determined in an ELISA reader using a 405 and 490 nm (reference) filters. Poly(ADP‐ribose)polymerase (PARP) was determined as previously reported 32, 33…”

Section: Methodsmentioning

confidence: 99%

“…Although well characterized osteoblast‐like cell cultures have been used as a suitable in vitro model to study the interaction of biomaterials and their degradation products with bone cells at the tissue–implant interface,28–31 other cell types may represent a good experimental model to test biocompatibility. In previous researches we have tested the biocompatibility of surface‐treated Ti‐6Al‐4V titanium alloy, using as experimental model both human umbilical vein endothelial cells (HUVEC)32 and human peripheral blood mononuclear cells (PBMC) 33. In fact, vascular endothelial cells, representing the interface between the blood‐stream and tissues, play several critical roles, including regulation of the adhesion of blood monocytes and their subsequent migration across the endothelium 34.…”

Section: Introductionmentioning

confidence: 99%

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Biocompatibility evaluation of surface‐treated AISI 316L austenitic stainless steel in human cell cultures

Martinesi

Bruni

Stio

et al. 2006

J Biomedical Materials Res

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Abstract:The effects of AISI 316L austenitic stainless steel, tested in untreated state or subjected to glow-discharge nitriding (at 10 or 20 hPa) and nitriding ϩ post-oxidizing treatments, on human umbilical vein endothelial cells (HUVEC) and on peripheral blood mononuclear cells (PBMC) were evaluated. All the treated samples showed a better corrosion resistance in PBS and higher surface hardness in comparison with the untreated alloy. In HUVEC put in contact for 72 h with the sample types, proliferation and apoptosis decreased and increased, respectively, in the presence of the nitrided ϩ post-oxidized samples, while only slight differences in cytokine (TNF-␣, IL-6, and TGF-␤1) release were registered. Intercellular adhesion molecule-1 (ICAM-1) increased in HUVEC incubated with all the treated samples, while vascular cell adhesion molecule-1 (VCAM-1) and E-selectin increased in the presence of all the sample types. PBMC incubated for 48 h with the samples showed a decrease in proliferation and an increase in apoptosis in the presence of the untreated samples and the nitrided ϩ post-oxidized ones. All the sample types induced a remarkable increase in TNF-␣ and IL-6 release in PBMC culture medium, while only the untreated sample and the nitrided at 10 hPa induced an increase in ICAM-1 expression. In HUVEC cocultured with PBMC, previously put in contact with the treated AISI 316L samples, increased levels of ICAM-1 were detected. In HUVEC coincubated with the culture medium of PBMC, previously put in contact with the samples under study, a noteworthy increase in ICAM-1, VCAM-1, and E-selectin levels was always registered, with the exception of VCAM-1, which was not affected by the untreated sample. In conclusion, even if the treated samples do not show a marked increase in biocompatibility in comparison with the untreated alloy, their higher corrosion resistance may suggest a better performance as the contact with physiological environment becomes longer.

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“…In previous researches we have tested the biocompatibility of surface‐treated Ti‐6Al‐4V titanium alloy and surface treated AISI 316L austenitic stainless steel, using as experimental model both Human Umbilical Vein Endothelial Cells (HUVEC) and Human Peripheral Blood Mononuclear Cells (PBMC) 35–37. The choice of HUVEC and PBMC cultures for verifying biocompatibility was justified by these considerations.…”

Section: Introductionmentioning

confidence: 99%

In vitro biocompatibility evaluation of surface‐modified titanium alloys

Treves

Martinesi

Stio

et al. 2009

J Biomedical Materials Res

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The present work is aimed to evaluate the effects of a surface modification process on the biocompatibility of three vanadium-free titanium alloys with biomedical applications interest. Chemical composition of alloys investigated, in weight %, were Ti-7Nb-6Al, Ti-13Nb-13Zr, and Ti-15Zr-4Nb. An easy and economic method intended to improve the biocompatibiblity of these materials consists in a simple thermal treatment at high temperature, 750 degrees C, in air for different times. The significance of modification of the surface properties to the biological response was studied putting in contact both untreated and thermally treated alloys with human cells in culture, Human Umbilical Vein Endothelial Cells (HUVEC) and Human Peripheral Blood Mononuclear Cells (PBMC). The TNF-alpha release data indicate that thermal treatment improves the biological response of the alloys. The notable enhancement of the surface roughness upon oxidation could be related with the observed reduction of the TNF-alpha levels for treated alloys. A different behavior of the two cell lines may be observed, when adhesion molecules (ICAM-1 and VCAM-1 in HUVEC, ICAM-1, and LFA-1 in PBMC) were determined, PBMC being more sensitive than HUVEC to the contact with the samples. The data also distinguish surface composition and corrosion resistance as significant parameters for the biological response.

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In vitro interaction between surface‐treated Ti‐6Al‐4V titanium alloy and human peripheral blood mononuclear cells

Cited by 20 publications

References 46 publications

Cytocompatibility and early inflammatory response of human endothelial cells in direct culture with Mg-Zn-Sr alloys

Cytocompatibility and early inflammatory response of human endothelial cells in direct culture with Mg-Zn-Sr alloys

Biocompatibility evaluation of surface‐treated AISI 316L austenitic stainless steel in human cell cultures

In vitro biocompatibility evaluation of surface‐modified titanium alloys

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