<i>In Vitro</i> Imaging and <i>in Vivo</i> Liver Targeting with Carbohydrate Capped Quantum Dots

Kikkeri, Raghavendra; Lepenies, Bernd; Adibekian, Alexander; Laurino, Paola; Seeberger, Peter H.

doi:10.1021/ja807711w

Cited by 225 publications

(159 citation statements)

References 36 publications

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“…Although different types of nanomaterials including CdS/ZnS quantum dots, [33][34][35][36][37][38] upconverting nanoparticles, 39 magnetic nanoparticles, [40][41][42][43][44][45] and carbon nanotubes, 46 have been used for the synthesis of glyconanoparticles, the intrinsic colour changes associated with the aggregation of noble metal nanoparticles have ensured that these nanoparticles are extensively used for the development of bioassays.…”

Section: Noble Metals Used To Synthesise the Nanoparticle Corementioning

confidence: 99%

Glyconanoparticles for colorimetric bioassays

Marín

Schofield

Field

et al. 2015

Analyst

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Carbohydrate molecules are involved in many of the cellular processes that are important for life. By combining the specific analyte targeting of carbohydrates with the multivalent structure and change of solution colour as a consequence of plasmonic interactions with the aggregation of metal nanoparticles, glyconanoparticles have been used extensively for the development of bioanalytical assays. The noble metals used to create the nanocore, the methodologies used to assemble the carbohydrates on the nanoparticle surface, the carbohydrate chosen for each specific target, the length of the tether that separates the carbohydrate from the nanocore and the density of carbohydrates on the surface all impact on the structural formation of metal based glyconanoparticles. This tutorial review highlights these key components, which directly impact on the selectivity and sensitivity of the developed bioassay, for the colorimetric detection of lectins, toxins and viruses.3

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Section: Noble Metals Used To Synthesise the Nanoparticle Corementioning

confidence: 99%

Glyconanoparticles for colorimetric bioassays

Marín

Schofield

Field

et al. 2015

Analyst

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“…9,34,35 The cytotoxicity of different DOX formulations was studied by MTT assay, which measures mitochondrial function or integrity.…”

Section: Discussionmentioning

confidence: 99%

Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma

Zhou¹,

Zhang²,

Yung³

et al. 2012

IJN

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Background: N-lactosyl-dioleoylphosphatidylethanolamine (Lac-DOPE) was synthesized and evaluated as a liver-specific targeting ligand via asialoglycoprotein receptors for liposomal delivery of doxorubicin. Methods: Lactosylated liposomes encapsulating calcein (Lac-L-calcein) or doxorubicin (Lac-L-DOX) composed of egg phosphatidylcholine, cholesterol, monomethoxy polyethylene glycol 2000-distearoyl phosphatidylethanolamine, and Lac-DOPE at 50:35:5:10 (mol/mol) were prepared by polycarbonate membrane extrusion and evaluated in human hepatocellular carcinoma HepG2 cells. Cellular uptake of Lac-L-calcein was monitored by confocal microscopy and by flow cytometry. The cytotoxicity of Lac-L-DOX was evaluated by MTT assay. The pharmacokinetic properties of Lac-L-DOX were studied in normal mice, and its biodistribution and antitumor activity were studied in nude mice with HepG2 xenografts. Results: The size of Lac-L-DOX was less than 100 nm and the liposomes demonstrated excellent colloidal stability. In vitro uptake of Lac-L-calcein by HepG2 cells was four times greater than that of non-targeted L-calcein. In the presence of 20 mM lactose, the uptake of Lac-L-calcein was inhibited, suggesting that asialoglycoprotein receptors mediated the observed cellular uptake. Lac-L-DOX exhibited enhanced in vivo cytotoxicity compared with the nontargeted liposomal doxorubicin (L-DOX), and its pharmacokinetic parameters indicate that Lac-L-DOX has a long blood circulation time (t 1/2 8.73 hours). Tissue distribution and therapeutic efficacy studies in nude mice bearing HepG2 xenografts show that Lac-L-DOX had significantly stronger tumor inhibitory activity compared with L-DOX and free doxorubicin, along with a higher accumulation of drug within the tumor site and greater cellular uptake by tumor cells. Conclusion: These data suggest that lactosylated liposomes are promising drug delivery vehicles for hepatocellular carcinoma.

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“…15 In active targeted therapy for HCC, galactose is considered to be a promising ligand because it can specifically recognize and bind asialoglycoprotein receptor, which is overexpressed exclusively on the surfaces of hepatic parenchymal cells. 16,17 Galactose has been conjugated to drug carriers to achieve targeted chemotherapeutic or diagnostic agent delivery to HepG2 cells overexpressing the asialoglycoprotein receptor, although the precise mechanism of asialoglycoprotein receptor-mediated uptake of galactose-modified carriers into HepG2 cells remains unresolved.…”

Section: Introductionmentioning

confidence: 99%

Galactosylated poly(ethylene glycol)-b-poly(l-lactide-co-ß-malic acid) block copolymer micelles for targeted drug delivery: preparation and in vitro characterization

Suo

Qian²,

Yao³

et al. 2010

IJN

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Biodegradable galactosylated methoxy poly(ethylene glycol)/poly(l-lactide-co-β-malic acid) (Gal-PEG-b-PLMA) block copolymer micelles were successfully prepared by a solvent diffusion method, and could efficiently encapsulate doxorubicin. The Gal-PEG-b-PLMA micelles before and after doxorubicin loading were characterized by size, morphology, in vitro drug release, and in vitro cytotoxicity in HepG2 cells. Transmission electron microscopy and dynamic light scattering results showed that the empty and doxorubicin-loaded micelles were approximately spherical in shape and had mean sizes of about 72 nm and 85 nm, respectively. In vitro release behavior of doxorubicin from the micelles was pH-dependent, with obviously faster release rates at mildly acidic pH 4.5 and 5.5 compared with physiologic pH 7.4. Methylthiazoletetrazolium assay and flow cytometric analysis indicated that the doxorubicin-loaded galactosylated micelles exhibited a greater growth-inhibitory effect on HepG2 cells than the nongalactosylated doxorubicin-loaded micelles, and induced S phase cell cycle arrest. Confocal laser scanning microscope observations revealed that the galactosylated micelles could be efficiently internalized by HepG2 cells through receptor-mediated endocytosis. The results suggest that Gal-PEG-b-PLMA copolymer micelles are a promising carrier system for targeted drug delivery in cancer therapy.

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In Vitro Imaging and in Vivo Liver Targeting with Carbohydrate Capped Quantum Dots

Abstract: PEGylated quantum dots (QDs) capped with d-mannose, d-galactose, and d-galactosamine have been synthesized. The stable, high quantum yield fluorescence of QDs was exploited to study specific carbohydrate-protein interactions in vitro and in vivo.

Cited by 225 publications

References 36 publications

Glyconanoparticles for colorimetric bioassays

Glyconanoparticles for colorimetric bioassays

Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma

Galactosylated poly(ethylene glycol)-b-poly(l-lactide-co-ß-malic acid) block copolymer micelles for targeted drug delivery: preparation and in vitro characterization

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In Vitro Imaging and in Vivo Liver Targeting with Carbohydrate Capped Quantum Dots

Abstract: PEGylated quantum dots (QDs) capped with d-mannose, d-galactose, and d-galactosamine have been synthesized. The stable, high quantum yield fluorescence of QDs was exploited to study specific carbohydrate-protein interactions in vitro and in vivo.

Cited by 225 publications

References 36 publications

Glyconanoparticles for colorimetric bioassays

Glyconanoparticles for colorimetric bioassays

Lactosylated liposomes for targeted delivery of doxorubicin to hepatocellular carcinoma

Galactosylated poly(ethylene glycol)-b-poly(l-lactide-co-&szlig;-malic acid) block copolymer micelles for targeted drug delivery: preparation and in vitro characterization

Contact Info

Product

Resources

About

Galactosylated poly(ethylene glycol)-b-poly(l-lactide-co-ß-malic acid) block copolymer micelles for targeted drug delivery: preparation and in vitro characterization