In Vitro
 Fungicidal Activities of Echinocandins against
 Candida metapsilosis
 ,
 C. orthopsilosis
 , and
 C. parapsilosis
 Evaluated by Time-Kill Studies

Cantón, Emilia; Espinel-Ingroff, Ana; Pemán, Javier; Castillo, Luis

doi:10.1128/aac.01538-09

Cited by 58 publications

(37 citation statements)

References 16 publications

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“…In 2005, Tavanti et al (48) suggested that the C. parapsilosis complex could be replaced by 3 different closely related species named C. parapsilosis sensu stricto, C. orthopsilosis, and C. metapsilosis. These species may show differences in antifungal susceptibilities and virulence, and an increased interest in the study of their epidemiology has arisen in the last years (8,19,23,31,34,46,47,51,52). Interestingly, the incidence of C. orthopsilosis and C. metapsilosis infections may have increased since 2004 (31), with prevalence rates ranging from 2.3 to 9% and 0.9 to 6.9%, respectively, depending on the geographical area and clinical specimens analyzed.…”

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confidence: 99%

Prospective Multicenter Study of the Epidemiology, Molecular Identification, and Antifungal Susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis Isolated from Patients with Candidemia

Cantón¹,

Pemán²,

Quindós

et al. 2011

Antimicrob Agents Chemother

138

111

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A 13-month prospective multicenter study including 44 hospitals was carried out to evaluate the epidemiology of Candida parapsilosis complex candidemia in Spain. Susceptibility to amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole, posaconazole, anidulafungin, caspofungin, and micafungin was tested by the microdilution colorimetric method. A total of 364 C. parapsilosis complex isolates were identified by molecular methods: C. parapsilosis (90.7%), Candida orthopsilosis (8.2%), and Candida metapsilosis (1.1%). Most candidemias (C. parapsilosis, 76.4%; C. orthopsilosis, 70.0%; C. metapsilosis, 100%) were observed in adults. No C. orthopsilosis or C. metapsilosis candidemias occurred in neonates. C. parapsilosis was most frequent in adult intensive care unit (28.8%), surgery (20.9%), and internal medicine (19.7%) departments; and C. orthopsilosis was most frequent in hematology (28.6%), pediatrics (12.0%), and neonatology (11.5%) departments. The geographic distribution of C. orthopsilosis and C. metapsilosis was not uniform. According to CLSI clinical breakpoints, all C. orthopsilosis and C. metapsilosis isolates were susceptible to the nine agents tested. Resistance (MICs > 1 mg/liter) was observed only in C. parapsilosis: amphotericin B, posaconazole, itraconazole, and caspofungin (0.3% each), anidulafungin (1.9%), and micafungin (2.5%). Applying the new species-specific fluconazole and echinocandin breakpoints, the rates of resistance to fluconazole for C. parapsilosis and C. orthopsilosis increased to 4.8% and 0.3%, respectively; conversely, for C. parapsilosis they shifted from 1.9 to 0.6% (anidulafungin) and from 2.5 to 0.6% (micafungin). Our study confirms the different prevalence of C. parapsilosis complex candidemia among age groups: neither C. orthopsilosis nor C. metapsilosis was isolated from neonates; interestingly, C. metapsilosis was isolated only from adults and the elderly. The disparity in antifungal susceptibility among species could be important for therapy.

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confidence: 99%

Prospective Multicenter Study of the Epidemiology, Molecular Identification, and Antifungal Susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis Isolated from Patients with Candidemia

Cantón¹,

Pemán²,

Quindós

et al. 2011

Antimicrob Agents Chemother

138

111

View full text Add to dashboard Cite

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“…They found C. parapsilosis most frequently in adult intensive care unit, surgery, and internal medicine departments, and in their study, most C. parapsilosis candidemias were observed in adults (4). Moreover, the new class of echinocandin broad-spectrum antifungals-although very active against C. albicans or C. glabrata-shows increased MICs against most clinical isolates of the C. parapsilosis group (15), with differences at the species level (3).…”

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confidence: 99%

“…Candida species are the cause of nosocomial sepsis, septic shock, and lethal disseminated infection in critically ill patients admitted to intensive care units (ICUs), rising over the past decade to the fourth most common pathogens causing nosocomial bloodstream infections in the United States (3). The increasing frequency rate of serious Candida hospital infections in intensive care patients has multifactorial causes, including increasing usage of intravascular catheters, broad-spectrum antibiotics, extensive surgery, and immunosuppression for neoplastic disease or allograft preservation (2,8).…”

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confidence: 99%

Microsatellite Genotyping Clarified Conspicuous Accumulation of Candida parapsilosis at a Cardiothoracic Surgery Intensive Care Unit

et al. 2012

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Candida parapsilosis has become a significant cause of invasive fungal infections in seriously ill patients. Nosocomial outbreaks through direct and indirect contact have been described. The aim of this study was the molecular characterization of what appeared to be an ongoing C. parapsilosis outbreak at the cardiothoracic intensive care unit of the University Hospital of Vienna between January 2007 and December 2008. Using two different molecular typing methods-automated repetitive sequencebased PCR (DiversiLab; bioMérieux) and microsatellite genotyping-we investigated the genetic relationship of 99 C. parapsilosis isolates. Eighty-three isolates originated from the cardiothoracic intensive care unit, while 16 isolates were random control isolates from other intensive care units and a different Austrian hospital. The 99 C. parapsilosis isolates analyzed by repetitiveelement PCR all showed identical genotypes, suggesting an ongoing outbreak. In contrast, microsatellite genotyping showed a total of 56 different genotypes. Two major genotypes were observed in 10 and 15 isolates, respectively, whereas another 13 genotypes were observed in 2 to 4 isolates each. Forty-one genotypes were observed only once. Closely related genotypes that differed in only a single microsatellite marker were grouped into clonal complexes. When it comes to C. parapsilosis, microsatellite genotyping is a more discriminative method than repetitive-element PCR genotyping to investigate outbreaks.

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“…In 2005, Tavanti et al suggested that the 3 groups of C. parapsilosis should be replaced by 3 different related species named C. parapsilosis sensu stricto, C. orthopsilosis, and C. metapsilosis (25). Since then, an increased interest in studying the epidemiology has arisen based on the observed differences in antifungal susceptibilities and virulence among the three species (4,6,7,10,15,17,19,23,24,28,29). Differentiating among the three species of the C. parapsilosis sensu lato group cannot be achieved by phenotypic methods.…”

mentioning

confidence: 99%

Assessment of Two New Molecular Methods for Identification of Candida parapsilosis Sensu Lato Species

García‐Effrón¹,

Cantón²,

Pemán³

et al. 2011

J Clin Microbiol

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Candida parapsilosis sensu stricto, C. orthopsilosis, and C. metapsilosis replaced C. parapsilosis groups I, II, and III in 2005. Since then, an increased interest in studying their epidemiology has arisen based on the observed differences in antifungal susceptibilities and virulence the three species. A strict differentiation of these species cannot be achieved by phenotypic methods. We evaluate two new molecular methodologies to differentiate among these species by the use of a collection of 293 bloodstream infection isolates of C. parapsilosis sensu lato. For the first method, the isolates were studied using PCR amplification of a fragment of the C. parapsilosis sensu lato FKS1 gene and a universal primer pair followed by EcoRI enzyme digestion. The other method used the allele discrimination ability of molecular beacons in a multiplex real-time PCR format. Both methods of identification showed 100% concordance with internal transcribed spacer 1 (ITS1)/ITS2 sequencing and proved to be effective for clinical applications, even with mixed-species DNAs.

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In Vitro Fungicidal Activities of Echinocandins against Candida metapsilosis , C. orthopsilosis , and C. parapsilosis Evaluated by Time-Kill Studies

Cited by 58 publications

References 16 publications

Prospective Multicenter Study of the Epidemiology, Molecular Identification, and Antifungal Susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis Isolated from Patients with Candidemia

Prospective Multicenter Study of the Epidemiology, Molecular Identification, and Antifungal Susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis Isolated from Patients with Candidemia

Microsatellite Genotyping Clarified Conspicuous Accumulation of Candida parapsilosis at a Cardiothoracic Surgery Intensive Care Unit

Assessment of Two New Molecular Methods for Identification of Candida parapsilosis Sensu Lato Species

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