<i>In vitro</i> Fertilizing Capacity of Frozen‐thawed Bull Spermatozoa Selected by Single‐layer (Glycidoxypropyltrimethoxysilane) Silane‐coated Silica Colloidal Centrifugation

Thys, Mirjan; Vandaele, Leen; Morrell, Jane M.; Mestach, J.; Soom, Ann Van; Hoogewijs, Maarten; Rodriguez‐Martinez, Heriberto

doi:10.1111/j.1439-0531.2008.01081.x

Cited by 55 publications

(68 citation statements)

References 16 publications

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“…In these parameters, the protocols using silica colloidal-silane (O4a and O1a) surpassed the protocols of silica colloidal-PVP selection. Similar results were reported in other studies conducted with bovines (Mendes et al, 2003;Samardzija et al, 2005;Thys et al, 2009), humans (Makkar et al, 1999 and equines (Rodríguez-Villamil et al, 2012), who compared selection methods employing silica colloidalsilane and silica colloidal-PVP. There was no significant difference in TM and PM between the two protocols using silica colloidal-silane (O4a and O1a).…”

Section: Discussionsupporting

confidence: 79%

“…In contrast to other authors (Samardzija et al, 2005;Machado et al, 2009;Mehmood et al, 2009;Alvarez et al, 2010), the present study did not report significant increase in TM and PM in the silica colloidal-PVP protocol using 4mL (P4) when compared to the samples before selection. These results can vary according to the concentration of colloidal silica solutions, gradient preparation (Thys et al, 2009), variations in the intensity of the g force and centrifugation time (Gil et al, 2000;Thys et al, 2009), variation of washing solutions after sperm selection, quality of sample before centrifugation (Martinez-Alborcia et al, 2013), among other reasons.…”

Section: Discussionmentioning

confidence: 99%

“…Studies with bovines (Mendes et al, 2003;Samardzija et al, 2005;Thys et al, 2009), equines (Gutierrez-Cepeda et al, 2011Rodríguez-Villamil et al, 2012), swine (Martinez-Alborcia et al, 2012;Bucci et al, 2013;Sjunnesson et al, 2013), dogs (Hishinuma e Sekine, 2004), goats (Jimenez-Rabadan et al,2012) and humans (Perez et al, 1997) reported an increase in the quality and viability of semen post selection in SLC or DLC. However, there are no studies that compare these two sperm selection methods in ovine.…”

Section: Introductionmentioning

confidence: 99%

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Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Bergstein

Bicudo

Rodello

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Frozen and thawed ovine semen undergo morphological and functional changes that prevent or decrease the efficiency of fertilization. Sperm selection methods seek to improve the quality and viability of the fertilizing materials. Four sperm selection methods were employed, using two silica colloidal solutions coated with silane (silica colloidal-silane) or by polyvinylpyrrolidone (silica colloidal-PVP), and varying the volume of colloidal solution. Sperm kinematic and sperm recovery were evaluated by means of CASA. The protocols using silica colloidal-silane showed higher total motility (TM), progressive motility (PM) and percentage of rapid sperm (%RAP) compared to the methods employing silica colloidal-PVP and to the samples prior to sperm selection. The silica colloidal-PVP had greater sperm recovery compared to the silica colloidal-silane. Only the method using 4mL of silica colloidal-PVP was not efficient in selecting samples with better quality compared to the samples analyzed prior to sperm selection. The methods using lower volumes of colloidal solution did not differ from those using higher volumes and the best results were shown by the method with 1mL silica colloidal-silane. The results found in the study indicated greater efficiency of the silica colloidal-silane solution for sperm selection of thawed ovine semen when compared to selection using silica colloidal-PVP. The method using 1mL of silica colloidal-silane was equally efficient to the method with higher volume, presenting itself as an alternative to process samples with lower sperm concentration.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Bergstein

Bicudo

Rodello

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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“…Recently, single layer centrifugation (SLC) using Androcoll TM (SLU, Uppsala, Sweden) has been successfully used for sperm selection in different animal species (Morrell et al, 2009e;Thys et al, 2009;Chatdarong et al, 2010). In this SLC technique, spermatozoa are centrifuged through a column (single layer) of silane-coated silica colloid in a species-specific formulation.…”

Section: Introductionmentioning

confidence: 99%

Effect of single layer centrifugation using Androcoll-E-Large on the sperm quality parameters of cooled-stored donkey semen doses

Ortiz

Dorado

Ramirez

et al. 2014

Animal

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The aim of this study was to determine the effect of single layer centrifugation (SLC) using Androcoll-E-Large on donkey sperm quality parameters after 24 h of cool-storage. Ejaculates were collected from Andalusian donkeys and then cooled at 5°C. SLC was carried out after 24 h of cool-storage using Androcoll-E-Large. In the first experiment, all sperm parameters assessed (total and progressive sperm motility, viability, sperm morphology and sperm kinematics VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) were statistically compared between semen samples processed or not with Androcoll-E-Large. Significant differences ( P < 0.05) were found between SLC-selected and unselected semen samples for all parameters assessed, obtaining better results after SLC. In the second experiment, semen samples were classified in two groups according to their sperm progressive motility (PM) before SLC. Then, the increments obtained in semen quality parameters after SLC were compared between groups. No significant differences were found between groups, indicating that SLC improved the sperm quality parameters of entire set of semen samples processed with independence to their original PM. In conclusion, SLC with Androcoll-E-Large can be used in donkeys, increasing the sperm quality of cooled-stored donkey semen doses after 24 h of cool storage.

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“…The pronuclei (PN) of presumptive zygotes were identified by epifluorescence microscopy (Leica DMR) at a magnification of Â400 (oil immersion). Zygotes with 2PN were considered normally fertilised, whereas those with .2PN were considered to be polyspermic (Thys et al 2009). …”

Section: Oocyte Ivm and Ivfmentioning

confidence: 99%

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

Rahman

Vandaele²,

Rijsselaere

et al. 2014

Reprod. Fertil. Dev.

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Abstract. Heat stress has long been recognised as a cause of subfertility in farm animals. The objectives of the present study were to elucidate the effect of heat stress on sperm function and involvement of the mitogen-activated protein kinase (MAPK) 14 signalling pathway. Spermatozoa incubated for 4 h at a physiological temperature (38.58C) exhibited significantly (P , 0.05) reduced motility, plasma membrane integrity and mitochondrial potential compared with nonincubated spermatozoa; the reductions in these parameters were more severe following incubation at a hyperthermic (418C) temperature (P , 0.01). Percentages of fertilisation and embryo development were highly affected in spermatozoa incubated at 418C compared with non-incubated spermatozoa (P , 0.01). Similarly, embryo quality was adversely affected by sperm incubation at 418C, as indicated by a higher apoptotic cell ratio in Day 7 blastocysts compared with that in the non-incubated control group (14.6% vs 6.7%, respectively; P , 0.01). Using SB203580 (10 mg mL À1 ), a specific inhibitor of the p38 MAPK pathway, during sperm hyperthermia reduced MAPK14 activation (24.9% vs 35.6%), increased sperm motility (45.8% vs 26.5%) and reduced DNA fragmentation (16.9% vs 23.4%) compared with the untreated control group, but did not improve subsequent fertilisation and embryo development. In conclusion, heat stress significantly affects the potential of spermatozoa to penetrate oocytes, as well as subsequent embryo development and quality. Notably, the data show that the MAPK14 signalling pathway is largely involved in heat-induced sperm damage. However, further research is needed to elucidate other signalling pathways possibly involved in heat-induced sperm damage.Additional keywords: functional parameters, mitogen-activated protein kinase 14 phosphorylation, terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling.

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In vitro Fertilizing Capacity of Frozen‐thawed Bull Spermatozoa Selected by Single‐layer (Glycidoxypropyltrimethoxysilane) Silane‐coated Silica Colloidal Centrifugation

Cited by 55 publications

References 16 publications

Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Effect of single layer centrifugation using Androcoll-E-Large on the sperm quality parameters of cooled-stored donkey semen doses

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

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