<i>In vitro</i> evaluation of the genotoxicity of CeO<sub>2</sub> nanoparticles in human peripheral blood lymphocytes using cytokinesis-block micronucleus test, comet assay, and gamma H2AX

Könen-Adıgüzel, Serpil; Ergene, Serap

doi:10.1177/0748233717753780

Cited by 23 publications

(17 citation statements)

References 43 publications

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“…Previous studies generally demonstrated that doses higher than those employed in the present study, i.e., 40, 80 or even 200 µg/mL depending on the cell type, are required to observe genotoxic effects evaluated by the comet assay on cell lines exposed to these NPs [ 33 , 39 , 46 ]. However, similarly to our findings, Auffan et al [ 47 ] and Könen-Adıgüzel and Ergene [ 48 ] obtained dose-dependent increases of DNA damage at very low doses of CeO 2 NPs employing human dermal fibroblasts and peripheral blood leucocytes, respectively, treated in vitro. With regard to the oxidative DNA damage, the positive responses observed in the current experiments for the three tested NPs are in agreement with previous studies employing FPG- or hOGG1-modified comet assays for evaluating TiO 2 NPs [ 17 , 49 , 50 ], ZnO NPs [ 16 , 19 ], and CeO 2 NPs [ 49 ].…”

Section: Discussionsupporting

confidence: 92%

Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

et al. 2021

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Metal oxide nanoparticles (NPs) have a wide variety of applications in many consumer products and biomedical practices. As a result, human exposure to these nanomaterials is highly frequent, becoming an issue of concern to public health. Recently, human salivary leucocytes have been proposed as an adequate non-invasive alternative to peripheral blood leucocytes to evaluate genotoxicity in vitro. The present study focused on proving the suitability of salivary leucocytes as a biomatrix in the comet assay for in vitro nanogenotoxicity studies, by testing some of the metal oxide NPs most frequently present in consumer products, namely, titanium dioxide (TiO2), zinc oxide (ZnO), and cerium dioxide (CeO2) NPs. Primary and oxidative DNA damage were evaluated by alkaline and hOGG1-modified comet assay, respectively. Any possible interference of the NPs with the methodological procedure or the hOGG1 activity was addressed before performing genotoxicity evaluation. Results obtained showed an increase of both primary and oxidative damage after NPs treatments. These data support the use of salivary leucocytes as a proper and sensitive biological sample for in vitro nanogenotoxicity studies, and contribute to increase the knowledge on the impact of metal oxide NPs on human health, reinforcing the need for a specific regulation of the nanomaterials use.

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Section: Discussionsupporting

confidence: 92%

Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

et al. 2021

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“…Similarly, the genotoxic effect of CONPs (<25 nm) was investigated on human lymphocyte cultures at concentrations range of 6, 12, and 18 mg/mL. It was reported that DNA damage occurred in the H2AX and Comet tests at 3 h and in the MN method at 24 h. There was no significant difference between concentrations when dose-related genotoxicity was evaluated, and the genotoxicity increased significantly at 72 h of exposure in all used tests (Könen-Adıgüzel and Ergene, 2018).…”

Section: Discussionmentioning

confidence: 99%

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

Arslan

Akbaba

2020

Toxicol Ind Health

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Cerium (IV) oxide (CeO2), which is used as a biomaterial, has wide application in areas such as the biomedical, glass polishing, electronic, automotive, and pharmacology industries. Comparing with the literature, in this study, the genotoxic effects of cerium (IV) oxide microparticles (COMPs) and cerium (IV) oxide nanoparticles (CONPs) were investigated for the first time in human peripheral blood cultures at concentrations of 0.78, 1.56, 3.125, 6.25, 12.5, 25, and 50 ppm for 72 h under in vitro conditions. Particle sizes of COMPs and CONPs were determined using scanning electron microscopic analysis. Micronucleus and chromosome aberration tests were used to determine the genotoxicity of COMPs and CONPs. The average particle sizes of COMPs and CONPs were approximately 148.25 and 25.30 nm, respectively. It was determined that CeO2 particles in both micro and nano sizes were toxic at all concentrations compared to the negative control group (distilled water). Importantly, COMPs and CONPs were genotoxic even at the lowest concentration (0.78 ppm). Comparing particle sizes, the data indicated that COMPs were more toxic than CONPs. The results suggest that genotoxicity of COMPs and CONPs may be a function of applied concentrations and particle sizes.

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“…However, several reports suggested that the uptake of nanoceria could induce oxidative stress and DNA damage, apoptosis, dephosphorylation of various substrates, aberrant cell signaling, and alterations in the transcriptional and posttranslational levels. 3,[30][31][32] Biomedical Relevance of Nanoceria Kailashiya, Dash…”

Section: Toxicity and Adverse Effectsmentioning

confidence: 99%

Nanoceria and Its Biomedical Relevance

Kailashiya

Dash

2019

Annals of the National Academy of Medical Sciences (India)

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Nanoceria is a nanosized particle preparation of cerium oxide. It shows mixture of cerium in the 3+ and 4+ states on the nanoparticle surface, giving it interesting redox properties. Nanoceria shows effective biological antioxidant properties, which makes it a great candidate for biomedical applications. Many studies have shown promising results on therapeutic potential of nanoceria in diseases like cancer, diabetes, atherosclerosis, and neurodegenerative diseases. Meanwhile, other studies explored biodistribution and toxicity of nanoceria. This review article describes nanoceria, its relevant biomedical applications, and adverse effects, based on previously reported studies.

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In vitro evaluation of the genotoxicity of CeO₂ nanoparticles in human peripheral blood lymphocytes using cytokinesis-block micronucleus test, comet assay, and gamma H2AX

Cited by 23 publications

References 43 publications

Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

Nanoceria and Its Biomedical Relevance

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In vitro evaluation of the genotoxicity of CeO2 nanoparticles in human peripheral blood lymphocytes using cytokinesis-block micronucleus test, comet assay, and gamma H2AX

Cited by 23 publications

References 43 publications

Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

Nanoceria and Its Biomedical Relevance

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In vitro evaluation of the genotoxicity of CeO₂ nanoparticles in human peripheral blood lymphocytes using cytokinesis-block micronucleus test, comet assay, and gamma H2AX