In vitro biocompatibility of a novel membrane of the composite poly(vinylidene‐trifluoroethylene)/barium titanate

Abstract

This study was aimed at investigating the in vitro biocompatibility of a novel membrane of the composite poly(vinylidene-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT). Osteoblastic cells were obtained from human alveolar bone fragments and cultured under standard osteogenic condition until subconfluence. First passaged cells were cultured on P(VDF-TrFE)/BT and expanded polytetrafluoroethylene (e-PTFE--control) membranes in 24-well plates. Cell adhesion and spreading were evaluated at 30 min, and 4 and 24 h. For proliferation assay, cells were cultured for 1, 7, and 10 days. Cell viability was detected by trypan blue at 7 and 10 days. Total protein content and alkaline phosphatase (ALP) activity were measured at 7, 14, and 21 days. Cultures were stained with Alizarin red at 21 days, for detection of mineralized matrix. Data were compared by ANOVA and Student t test. Cell attachment (p = 0.001), cell number (p = 0.001), and ALP activity (p = 0.0001) were greater on P(VDF-TrFE)/BT. Additionally, doubling time was greater on P(VDF-TrFE)/BT (p = 0.03), indicating a decreased proliferation rate. Bone-like nodule formation took place only on P(VDF-TrFE)/BT. The present results showed that both membranes are biocompatible. However, P(VDF-TrFE)/BT presented a better in vitro biocompatibility and allowed bone-like nodule formation. Therefore, P(VDF-TrFE)/BT could be an alternative membrane to be used in guided tissue regeneration.

Applications of Piezoelectricity in Nanomedicine

“…In vitro results showed an improved adhesion of human osteoblast-like cells on poly(vinylidene-trifluoroethylene)/barium titanate [P(VDF-TrFE)-BaTiO 3 ], in comparison to a non-piezoelectric polymeric control [56]. In the piezoelectric material, alkaline phosphatase (ALP) activity and matrix mineralisation were also higher than those of controls.…”

On mechanical properties and bioactivity of PVDF-BCP composites

“…This arrangement promotes a constant ionic exchange between the biomaterial surface and the osteoinductive medium or animal body, inducing and accelerating the formation of an HA layer, catalyzing the curing process. In the last years many studies with electrically polarized hydroxyapatite (HA) [20], BaTiO 3 (BT) [21], polyvinylidene fluoride (PVDF) [22], hydroxyapatitebarium titanate (HABT) [23], and poly(vinylidenefluorideco-trifluoroethylene)-barium titanate [P(VDF-TrFE)-BaTiO 3 ] [24] composites were conducted in in vitro and in vivo experiments. The obtained results indicated a higher bioactivity for ceramics and polarized composites in comparison with non-polarized ones.…”

Structure–Property Relationships of Nanocomposites

“…They combine the strong ferroelectric and dielectric properties of ceramics with the easy processing and good mechanical properties of polymers. Dispersion of micrometer-sized ferroelectric particles in an electrically passive epoxy matrix was first published by Furukawa et al [1976] and later extended to ferroelectric matrices such as poly(vinylidene fluoride) (PVDF) and poly(vinylidene fluoride-co-3-fluoroethylene) (PVDF-TrFE) [Hsiang et al, 2001;Hilczer et al, 2002;Gimenes et al, 2004;Beloti et al, 2006]. However, the necessity of miniaturization of electronic components and the synergy required between the electrical and mechanical properties of thin films for transducer applications imply that nanoparticles should be used.…”