In VitroAntioxidant Activity and Phenolic Content ofCedrus brevifoliaBark

Abstract

A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The antioxidant activity was investigated using in vitro assays: DPPH and ABTS radicals scavenging and reducing power assays. The ethyl acetate fraction had the highest total phenolic and proanthocyanidin contents; a taxifolin-O-hexoside, catechin, epicatechin and procyanidin oligomers (three dimers, two trimers) were identified in this fraction. The ethyl acetate fraction was found to possess the highest DPPH and ABTS radicals scavenging effects (EC50=13.9 ± 0.3 and 2.3 ± 0.0 μg/mL, respectively) and reducing capacity (EC50=9.1 ±0.1 μg/mL). Antioxidant effects were highly correlated with total phenolic and proanthocyanidin contents (r=0.89-0.99). These results suggest that Cedrus brevifolia bark is a new source of antioxidants.

Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of Cedrus brevifolia Henry Extracts

“…This is confirmed with TEAC values which were calculated by comparing antioxidant activity of each extract to Trolox. These data suggest that the bark is the primary source of antioxidant constituents in C. brevifolia, a finding which is in agreement with previously reported data on the antioxidant activity of crude extracts and the phenolic content of the bark of C. brevifolia [13,33].…”

Vernonia kotschyana Roots: Therapeutic Potential via Antioxidant Activity

“…The ABTS scavenging activity of ethyl acetate extract was comparable to or better than that reported for different extracts of V. cinerea whole plant (EC 50 = 19.54–26.81 µg/mL) but inferior to that of ethanol extract of V. cinerea leaves (EC 50 = 12 µg/mL) [24,25]. There are also reports on extracts from other species ( Cedrus brevifolia , Angelica sinensis ) having higher ABTS scavenging potential (EC 50 = 2.3–11.9 µg/mL) [26,27]. As glutathione is a powerful antioxidant, it is obvious that ethyl acetate extract, approximately six times less potent, is an efficient ABTS radical scavenger.…”

Re-evaluation of ABTS•+ Assay for Total Antioxidant Capacity of Natural Products

“…2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS+), a stable free radical, is frequently used for estimating the total antioxidant capacity (TAC) of natural products, including crude extracts [5][6][7][8][9], polyphenols [10], phenolic acids [11], flavonoids [12], and others [13]. The original ABTS+ radical-scavenging assay was measured according to the activation of metmyoglobin with hydrogen peroxide in the presence of ABTS to produce the radical cation, in the presence or absence of antioxidants [14].…”