<i>In vitro</i> antimicrobial effect of bacteriophages on human dentin infected with <i>Enterococcus faecalis</i> ATCC 29212

Paisano, Adriana Fernandes; Spira, Beny; Cai, Silvana; Bombana, Antônio Carlos

doi:10.1111/j.1399-302x.2004.00166.x

Cited by 47 publications

(39 citation statements)

References 21 publications

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“…Although several bacteriophages have been isolated for a number of oral bacteria (8,10,12,13,23,33), there have been no reports on the isolation of a phage for fusobacteria until now. F. nucleatum, together with a bacterial consortium, generates the well-known dental plaque, a biofilm conformation that eventually leads to the onset of periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

“…There are many examples of the use of bacteriophages at a clinical (14,32) and commercial level (20). Specifically in the dentistry area, several bacteriophages that infect diverse oral bacteria have been isolated from saliva and dental plaque (12,13,23,37). Although F. nucleatum is an important periodontal pathogen, reports of bacteriophages for this microorganism do not exist.…”

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confidence: 99%

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Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum

Machuca

Daille

Vinés

et al. 2010

Appl Environ Microbiol

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Fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. We isolated a new bacteriophage (Fnp⌽02) that specifically infects this bacterium. Transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. The size of the phage genome was estimated to be approximately 59 kbp of double-stranded DNA. The morphological features and the genetic characteristics suggest that Fnp⌽02 is part of the Siphoviridae family. Using one-step growth and adsorption experiments, the latent period, burst size, and adsorption rate were estimated to be 15 h, 100 infectious units per cell, and 7.5 ؋ 10 ؊10 ml min ؊1 , respectively. A small fragment of phage DNA was cloned and sequenced, showing 93% nucleotide identity with the phage PA6 of Propionibacterium acnes and amino acid identity with fragments of two proteins (Gp3 and Gp4) of this phage. To our knowledge, Fnp⌽02 is the first phage described to infect Fusobacterium nucleatum and provides the base for future exploration of phages in the control of periodontal disease.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum

Machuca

Daille

Vinés

et al. 2010

Appl Environ Microbiol

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“…Despite the significance of E. faecalis in root canal infections, very few attempts were made to treat E. faecalis infection using phage therapy (31,32). In the present study, an E. faecalis-infecting phage was isolated from sewage and characterized genetically by sequencing and functionally by evaluating its efficacy against E. faecalis planktonic and biofilm cultures in vitro.…”

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confidence: 99%

Targeting Enterococcus faecalis Biofilms with Phage Therapy

Khalifa

Brosh

Gelman

et al. 2015

Appl Environ Microbiol

188

158

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Phage therapy has been proven to be more effective, in some cases, than conventional antibiotics, especially regarding multidrug-resistant biofilm infections. The objective here was to isolate an anti-Enterococcus faecalis bacteriophage and to evaluate its efficacy against planktonic and biofilm cultures. E. faecalis is an important pathogen found in many infections, including endocarditis and persistent infections associated with root canal treatment failure. The difficulty in E. faecalis treatment has been attributed to the lack of anti-infective strategies to eradicate its biofilm and to the frequent emergence of multidrug-resistant strains. To this end, an anti-E. faecalis and E. faecium phage, termed EFDG1, was isolated from sewage effluents. The phage was visualized by electron microscopy. EFDG1 coding sequences and phylogeny were determined by whole genome sequencing (GenBank accession number KP339049), revealing it belongs to the Spounavirinae subfamily of the Myoviridae phages, which includes promising candidates for therapy against Gram-positive pathogens. This analysis also showed that the EFDG1 genome does not contain apparent harmful genes. EFDG1 antibacterial efficacy was evaluated in vitro against planktonic and biofilm cultures, showing effective lytic activity against various E. faecalis and E. faecium isolates, regardless of their antibiotic resistance profile. In addition, EFDG1 efficiently prevented ex vivo E. faecalis root canal infection. These findings suggest that phage therapy using EFDG1 might be efficacious to prevent E. faecalis infection after root canal treatment.

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“…This may have affected the ability to recover viable bacteria post-treatment for phage efficacy quantification. Studies that had used successfully longer or multiple treatment times (129,130,132) may have used a variant phage. The 24h phage treatment time used in this study allowed for comparison with other research that also used this interaction period (37,39,40,42,132,149).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Bacteriophage Treatment on Pseudomonas aeruginosa Biofilms

Phee

Bondy-Denomy

Kishen

et al. 2013

Journal of Endodontics

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Introduction: Bacterial viruses (phages) have been used successfully in the treatment of animal and human bacterial infections. This study examined the potential use of phage therapy against Pseudomonas aeruginosa strain PA14 biofilms in a root canal model. were treated with phages identified with potential biofilm-degrading activities. Posttreatment intra-canal samples using paper points and round burs were taken to assess phage and bacterial counts. Results: Part 1: We identified two phages (JBD4 and JBD44a) with putative biofilm degrading activities. Treatment of PA14 biofilms with these phages produced a significant reduction in the mean percentage of biomass in 24h (p<0.05) and 96h (p=0.08) biofilms. Part 2: In 24 and 96h PA14 biofilms in a root canal model, no significant difference was found in the number of colony forming units after phage treatment (p>0.05). Conclusion: Phage application significantly reduced the biomass of 24 and 96h PA14 biofilms grown on microplates, but did not produce significant reduction of 24 or 96h PA14 biofilms grown in the extracted tooth model.iii

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In vitro antimicrobial effect of bacteriophages on human dentin infected with Enterococcus faecalis ATCC 29212

Cited by 47 publications

References 21 publications

Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum

Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum

Targeting Enterococcus faecalis Biofilms with Phage Therapy

Efficacy of Bacteriophage Treatment on Pseudomonas aeruginosa Biofilms

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