<i>In vitro</i>and<i>in vivo</i>studies of a gelatin/carboxymethyl chitosan/LAPONITE® composite scaffold for bone tissue engineering

Li, Tao; Liu, Zhonglong; Xiao, Ming; Yang, Zezheng; Liu, Zhiyuan; Zhou, Xiaojun; Wang, Jinwu

doi:10.1039/c7ra06913h

Cited by 79 publications

(48 citation statements)

References 67 publications

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“…The decreasing of swelling degree with the increased of Lap content can be attributed to the strong ionic interactions between chitosan and Lap, bolstering the polymer nanocomposite network interactions when submersed in PBS which reduce the swelling degree (Gaharwar, Schexnailder, Kline, & Schmidt, ). The same results were obtained in other studies in which the presence of clay nanoparticles decreased the swelling capacity of the polymer nanocomposite (Gaharwar et al, ; Kabiri, Mirzadeh, & Zohuriaan‐Mehr, ; Tao et al, ). Overall, the data exhibited that the scaffolds' swelling capacity depends on the clay amount.…”

Section: Discussionsupporting

confidence: 86%

“…It has a type 2:1 lamellar structure (two tetrahedral sheets and an octahedral center) in the form of disks with approximately 25 nm in diameter and 1 nm in thickness (Hill, Zhang, & Whitten, ). The silicate can act as cross‐linker in polymer fabrication, the incorporation of Lap in the polymer alters its characteristics such as hydration, dissolution, besides the mechanical properties (Tao et al, ). Therefore, Lap is considered as the next generation of bioactive materials, not only because of its unique biological properties but also due to its advantages such as high purity, low cost, and easy processing (Ordikhani, Dehghani, & Simchi, ).…”

Section: Introductionmentioning

confidence: 99%

“…Plethora of studies have been developed in biomedical and biotechnological area on the formation and characterization of nanocomposites scaffolds based on chitosan/clay for application in tissue engineering, bone regeneration, wound dressings, among others (Li et al, ; Sandri et al, ; Tao et al, ). Some techniques include solvent casting and particulate leaching, gas foaming, electrospinning, and emulsion freeze‐drying.…”

Section: Introductionmentioning

confidence: 99%

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Chitosan‐laponite nanocomposite scaffolds for wound dressing application

et al. 2019

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The pivotal issue of skin regeneration research is the development of effective biomaterials that exhibit biological activities as fungicide and bactericide, combining simple and low cost manufacturing technologies. In this context, nanocomposite scaffolds based on chitosan (Ch)/Laponite (Lap) were produced by using different concentrations of Lap via freeze‐drying process for potential application in skin regeneration. The influence of Lap concentration on the scaffold properties was evaluated. The prepared scaffolds were characterized by x‐ray diffraction (XRD), scanning electron microscopy (SEM), porosity, swelling capacity, and mechanical analyses. The results revealed that the scaffolds exhibited a porous architecture, besides the increase in the clay content, leads to an increase in the porosity, an improvement of mechanical strength, and a decrease of swelling capacity. In vitro tests were also carried out to evaluate the biocompatibility of the materials, such as bioadhesion, antibacterial activity, viability, and cell adhesion. Viability and cell adhesion demonstrated that all scaffolds were not cytotoxic and the fibroblast cells readily attached on the surface of the scaffolds. Thereby, the results suggested that the nanocomposite scaffolds are biomaterials potentially useful as wound dressings.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chitosan‐laponite nanocomposite scaffolds for wound dressing application

et al. 2019

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“…3 Thus, bone tissue engineering has attracted increasing interest for constructing biological substitutes that allow growth and proliferation of host cells in injured and diseased bone to restore and maintain normal function. [4][5][6][7] Mesenchymal stem cells (MSCs), as a main source of adult stem cells, have found numerous applications in tissue engineering and cell therapy due to their ability to selfreproduce and differentiate into a range of cells and tissues such as bone, cartilage, muscle, tendon, ligament, and fat. [8][9][10][11] MSCs have been the focus of recent studies in bone tissue engineering, in which they are seeded in a scaffold and induced to generate new osteogenic cells via osteoinductive cues.…”

Section: Introductionmentioning

confidence: 99%

β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

et al. 2018

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“…Lap has been reported to promote cell adhesion and proliferation and can exert osteogenic effects on cells in vitro 42. Consequently, Lap has been combined with macromolecules, such as DNA43 or proteins44 to fabricate hydrogels capable of promoting osteogenic differentiation in vitro45,46 or bone regeneration in vivo in mice 44…”

Section: Introductionmentioning

confidence: 99%

Growth‐Factor Free Multicomponent Nanocomposite Hydrogels That Stimulate Bone Formation

Okesola

Derkuş

et al. 2020

Adv Funct Materials

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Synthetic osteo‐promoting materials that are able to stimulate and accelerate bone formation without the addition of exogenous cells or growth factors represent a major opportunity for an aging world population. A co‐assembling system that integrates hyaluronic acid tyramine (HA‐Tyr), bioactive peptide amphiphiles (GHK‐Cu2+), and Laponite (Lap) to engineer hydrogels with physical, mechanical, and biomolecular signals that can be tuned to enhance bone regeneration is reported. The central design element of the multicomponent hydrogels is the integration of self‐assembly and enzyme‐mediated oxidative coupling to optimize structure and mechanical properties in combination with the incorporation of an osteo‐ and angio‐promoting segments to facilitate signaling. Spectroscopic techniques are used to confirm the interplay of orthogonal covalent and supramolecular interactions in multicomponent hydrogel formation. Furthermore, physico‐mechanical characterizations reveal that the multicomponent hydrogels exhibit improved compressive strength, stress relaxation profile, low swelling ratio, and retarded enzymatic degradation compared to the single component hydrogels. Applicability is validated in vitro using human mesenchymal stem cells and human umbilical vein endothelial cells, and in vivo using a rabbit maxillary sinus floor reconstruction model. Animals treated with the HA‐Tyr‐HA‐Tyr‐GHK‐Cu2+ hydrogels exhibit significantly enhanced bone formation relative to controls including the commercially available Bio‐Oss.

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In vitroandin vivostudies of a gelatin/carboxymethyl chitosan/LAPONITE® composite scaffold for bone tissue engineering

Abstract: In the present study, we fabricated a biocomposite scaffold composed of carboxymethyl chitosan (CMC), gelatin and LAPONITE® (Lap) nanoparticles via freeze-drying and investigated its potential use in bone tissue engineering.

Cited by 79 publications

References 67 publications

Chitosan‐laponite nanocomposite scaffolds for wound dressing application

Chitosan‐laponite nanocomposite scaffolds for wound dressing application

β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

Growth‐Factor Free Multicomponent Nanocomposite Hydrogels That Stimulate Bone Formation

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