In Vitro and In Vivo Evaluation of Microporous Chitosan Hydrogel/Nanofibrin Composite Bandage for Skin Tissue Regeneration

Abstract

In this work, we have developed chitosan hydrogel/nanofibrin composite bandages (CFBs) and characterized using Fourier transform-infrared spectroscopy and scanning electron microscopy. The homogeneous distribution of nanofibrin in the prepared chitosan hydrogel matrix was confirmed by phosphotungstic acid-hematoxylin staining. The mechanical strength, swelling, biodegradation, porosity, whole-blood clotting, and platelet activation studies were carried out. In addition, the cell viability, cell attachment, and infiltration of the prepared CFBs were evaluated using human umbilical vein endothelial cells (HUVECs) and human dermal fibroblast (HDF) cells. It was found that the CFBs were microporous, flexible, biodegradable, and showed enhanced blood clotting and platelet activity compared to the one without nanofibrin. The prepared CFBs were capable of absorbing fluid and this was confirmed when immersed in phosphate buffered saline. Cell viability studies on HUVECs and HDF cells proved the nontoxic nature of the CFBs. Cell attachment and infiltration studies showed that the cells were found attached and proliferated on the CFBs. In vivo experiments were carried out in Sprague-Dawley rats and found that the wound healing occurred within 2 weeks when treated with CFBs than compared to the bare wound and wound treated with Kaltostat. The deposition of collagen was found to be more on CFB-treated wounds compared to the control. The above results proved the use of these CFBs as an ideal candidate for skin tissue regeneration and wound healing.

Utilização de nanopartículas no tratamento de feridas: revisão sistemática

“…In the in vitro analysis of these nanoparticles, an increase in calcium absorption of the fibroblasts and an increase in the proliferation rate of fibroblasts was observed (26) . Finally, the results of study A12, performed using chitosan hydrogel mixed fibrin nanoparticles (CFBs), through in vivo and in vitro tests, reinforced the evidence of the contribution of the use of nanoparticle-based products to accelerate the process of blood coagulation, promotion of platelet activation and wound healing (27) . The advantage of using fibrin in nanoformulation is due to the ideal biomimetic matrix, in which the active portions of fibrin are ready for platelet activation, thus inducing a rapid healing process (27) .…”

“…Finally, the results of study A12, performed using chitosan hydrogel mixed fibrin nanoparticles (CFBs), through in vivo and in vitro tests, reinforced the evidence of the contribution of the use of nanoparticle-based products to accelerate the process of blood coagulation, promotion of platelet activation and wound healing (27) . The advantage of using fibrin in nanoformulation is due to the ideal biomimetic matrix, in which the active portions of fibrin are ready for platelet activation, thus inducing a rapid healing process (27) . At the end of this discussion, the results of the studies suggest evidence that nanoparticle-based dressings are effective and beneficial.…”

“…Kumar et al 2013 (27) In vitro and in vivo evaluation of microporous chitosan hydrogel/ nanofibrin composite bandage for skin tissue regeneration…”

Regenerative nanomedicine: current perspectives and future directions

“…In vivo studies on Sprague-Dawley rats using this hydrogel have shown faster wound healing response evidenced by increased collagen deposition. 51 Hydrogels are also effective in the controlled release of impregnated drugs, which is essential for the sustained stimulation of tissue growth. An interesting study by Jaiswal et al has shown that poly(vinyl alcohol) hydrogel impregnated with epigallocatechin gallate is very effective in healing wounds by improving angiogenesis, restoring epithelialization, and decreasing the inflammatory response.…”

Cytotoxic and cytostatic side effects of chitosan nanoparticles as a non-viral gene carrier

“…Then, cells were washed and suspended in PBS containing 0.1% glucose, 0.5 mM EDTA and 5 mM 2 0 ,7 0 -dichlorodihydrofluorescein diacetate (H 2 DCF-DA) (Sudheesh Kumar et al, 2013). Fluorescence light was monitored in a Tecan Infinite 1 M200 absorbance mode microplate reader at 495 nm (measurement wavelength) and at 525 nm (reference wavelength) (Mytych et al, , 2015b.…”