In silico analysis of kiss2, expression studies and protein–protein interaction with gonadotropin-releasing hormone 2 (GnRH2) and luteinizing hormone beta (LHβ) in Heteropneustes fossilis

Abstract

Supplementary file 2 (a): The High Ambiguity Driven protein-protein Docking (HADDOCK) docked clusters of kiss2-GnRH2 plotted against their RMSDs based on water defined models. The color codes of clusters represent interface-ligand RMSD values of all 20 docked clusters. (A and B) represents HADDOCK score plotted against their RMSDs where i-RMSD and I-RMSD calculated on backbone (C, N, CA, P, O) atoms of all residues involved in 10 A° cutoff intermolecular contacts. (C) Represents fraction of common contacts (FCC) against HADDOCK score, where 5 A° cutoff was used to determine intermolecular contacts. (D) Ambiguous Interaction Restraints (AIR) energy versus interface-RMSD plot of all residues involved in kiss2-GnRH2 docked complex. (E) The HADDOCK score corresponds to the weighted sum of intermolecular electrostatic (F) Van der Waals contacts plotted against i-RMSD.

Effect of exogenously administered thyroid hormones on gonadotropin, thyrotropin and deiodinases encoding genes in the catfish, Heteropneustes fossilis (Bloch)

“…A small piece of ovarian tissue was fixed in Bouin's fixative and processed for histological examination using hematoxylin-eosin staining. The brain with pituitary was excised and processed for total RNA isolation, followed by cDNA preparation as per our laboratory standard protocol (Kumari et al, 2020) . The primer pairs for the genes tsh-b, tsh-r, dio2, dio3, gnrh2, fsh-b and lh-b used for the quantitative analysis are mentioned in the table 1.…”

Multifactorial control of gonadotropin release for induction of oocyte maturation: Influence of gonadotropin-releasing hormone, gonadotropin release-inhibiting factor and dopamine receptors in the catfish, Heteropneustes fossilis

“…Diluted RNA samples were used to assess the quantity and quality of extracted RNA. Thereafter, PCR, cDNA cloning and sequencing of D2 and D4 receptor were performed (Kumari et al, 2020). The D2 and D4 receptor transcripts were analyzed by PRALINE online server and MEGA software version 6 (Kumar et al, 2018).…”

“…The D2 and D4 receptor transcripts were analyzed by PRALINE online server and MEGA software version 6 (Kumar et al, 2018). The specific primers were designed, and mRNA expression of D2 and D4 and lhb was quantified by using Power SYBR™ Green PCR master mix (Thermo Fisher scientific, USA) in 10 µl reaction volume (for details, see Kumari et al,2020) and the reaction was run on 7500 Applied Biosystem Fast DxReal-time PCR. Both sample and reference (β-actin) were run in duplicates.…”

A mini-review on kisspeptin hormone as an inducing agent in fish breeding

“…At the onset of sexual maturation in the threespine stickleback, kiss2 and kissr mRNA levels were higher, suggesting their possible involvement in pubertal onset (Shao et al, 2019) [67] . Likewise, changes in expression patterns of kiss2 mRNA during different developmental stages indicated its potential role in embryonic development of singhi, a freshwater catfish of India (Kumari et al, 2020) [28] .…”

“…Surprisingly, dibasic amino acid residues are located five and six positions upstream to Kiss2-10 in few species (Table 3). In zebrafish, goldfish, catla, rohu, golden mahseer, singhi and Chinese rare minnow, dibasic residues are located five position upstream to Kiss2-10 region, suggesting possibility of Kiss2 pentadecapeptide (Kiss2-15) as mature form in these species (Kitahashi et al, 2009;Li et al, 2009;Rather et al, 2016;Saha et al, 2016;Shahi et al, 2017;Kumari et al, 2020) [26,30,55,56,64,28] . In the rainbow trout, dibasic amino acid residues are found six position upstream to Kiss2-10 region, indicating possibility of Kiss2 hexadecapeptide as mature form (Genbank Accession No.…”