<i>dTrf2</i> is required for transcriptional and developmental responses to ecdysone during <i>Drosophila</i> metamorphosis

Bashirullah, Arash; Lam, Geanette; Yin, Viravuth P.; Thummel, Carl S.

doi:10.1002/dvdy.21350

Cited by 20 publications

(18 citation statements)

References 30 publications

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“…Even though males reared in silence did not mature more quickly than their counterparts reared with recorded calling, males from the silent treatment overexpressed two genes positively associated with moulting, TATA box binding protein-related factor 2 which responds to ecdysone during the onset of moulting [64] and ftz transcription factor 1 which is necessary for proper moulting through activation of ecdysone receptors by juvenile hormone [65]. It is interesting that these genes are expressed in males from the silent treatment as females from the silent treatment matured most quickly (Fig.…”

Section: Resultsmentioning

confidence: 99%

Australian black field crickets show changes in neural gene expression associated with socially-induced morphological, life-history, and behavioral plasticity

2016

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BackgroundEcological and evolutionary model organisms have provided extensive insight into the ecological triggers, adaptive benefits, and evolution of life-history driven developmental plasticity. Despite this, we still have a poor understanding of the underlying genetic changes that occur during shifts towards different developmental trajectories. The goal of this study is to determine whether we can identify underlying gene expression patterns that can describe the different life-history trajectories individuals follow in response to social cues of competition. To do this, we use the Australian black field cricket (Teleogryllus commodus), a species with sex-specific developmental trajectories moderated by the density and quality of calls heard during immaturity. In this study, we manipulated the social information males and females could hear by rearing individuals in either calling or silent treatments. We next used RNA-Seq to develop a reference transcriptome to study changes in brain gene expression at two points prior to sexual maturation.ResultsWe show accelerated development in both sexes when exposed to calling; changes were also seen in growth, lifespan, and reproductive effort. Functional relationships between genes and phenotypes were apparent from ontological enrichment analysis. We demonstrate that increased investment towards traits such as growth and reproductive effort were often associated with the expression of a greater number of genes with similar effect, thus providing a suite of candidate genes for future research in this and other invertebrate organisms.ConclusionsOur results provide interesting insight into the genomic underpinnings of developmental plasticity and highlight the potential of a genomic exploration of other evolutionary theories such as condition dependence and sex-specific developmental strategies.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3119-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Australian black field crickets show changes in neural gene expression associated with socially-induced morphological, life-history, and behavioral plasticity

2016

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“…84 Hypomorphic trf2 mutations display defects in major ecdysone-triggered biological responses, including puparium formation, anterior spiracle eversion, gas bubble translocation, adult head eversion, and larval salivary gland cell death. Drosophila trf2 appears to be required for the proper timing and levels of ecdysone-regulated gene expression required for entry into metamorphosis.…”

Section: Tbp-related Factorsmentioning

confidence: 99%

“…Drosophila trf2 appears to be required for the proper timing and levels of ecdysone-regulated gene expression required for entry into metamorphosis. 84 Additional support for the involvement of TRF2 in Drosophila metamorphosis comes from RNAi depletion of TRF2 in larval salivary glands that results in a significant reduction in the sizes of the cells and the glands. 71 Although these mutant embryos develop to the third instar larval stage, a majority of them fail to pupate or die during pupal stages.…”

Section: Tbp-related Factorsmentioning

confidence: 99%

TRF2: TRansForming the view of general transcription factors

et al. 2015

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These authors contributed equally to this work.Keywords: core promoter elements/motifs, DPE, embryonic development, histone gene cluster, RNAP II transcription, ribosomal protein genes, spermiogenesis, TBP-related factors, TRF2, TCT Transcriptional regulation is pivotal for development and differentiation of organisms. Transcription of eukaryotic protein-coding genes by RNA polymerase II (RNAP II) initiates at the core promoter. Core promoters, which encompass the transcription start site, may contain functional core promoter elements, such as the TATA box, initiator, TCT and downstream core promoter element. TRF2 (TATA-box-binding protein-related factor 2) does not bind TATA box-containing promoters. Rather, it is recruited to core promoters via sequences other than the TATA box. We review the recent findings implicating TRF2 as a basal transcription factor in the regulation of diverse biological processes and specialized transcriptional programs.

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“…that TRF2 is important for embryonic development and differentiation (Dantonel et al 2000;Kaltenbach et al 2000;Veenstra et al 2000;Martianov et al 2001;Zhang et al 2001;Bashirullah et al 2007;Zhou et al 2013). Furthermore, TRF2 was also implicated in DNA replication and proliferation (Hochheimer et al 2002).…”

Section: Trf2 Is Enriched In Protein Fractions Supporting Dpe Transcrmentioning

confidence: 99%

Drosophila TRF2 is a preferential core promoter regulator

et al. 2014

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Transcription of protein-coding genes is highly dependent on the RNA polymerase II core promoter. Core promoters, generally defined as the regions that direct transcription initiation, consist of functional core promoter motifs (such as the TATA-box, initiator [Inr], and downstream core promoter element [DPE]) that confer specific properties to the core promoter. The known basal transcription factors that support TATA-dependent transcription are insufficient for in vitro transcription of DPE-dependent promoters. In search of a transcription factor that supports DPE-dependent transcription, we used a biochemical complementation approach and identified the Drosophila TBP (TATA-boxbinding protein)-related factor 2 (TRF2) as an enriched factor in the fractions that support DPE-dependent transcription. We demonstrate that the short TRF2 isoform preferentially activates DPE-dependent promoters. DNA microarray analysis reveals the enrichment of DPE promoters among short TRF2 up-regulated genes. Using primer extension analysis and reporter assays, we show the importance of the DPE in transcriptional regulation of TRF2 target genes. It was previously shown that, unlike TBP, TRF2 fails to bind DNA containing TATA-boxes. Using microfluidic affinity analysis, we discovered that short TRF2-bound DNA oligos are enriched for Inr and DPE motifs. Taken together, our findings highlight the role of short TRF2 as a preferential core promoter regulator.

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dTrf2 is required for transcriptional and developmental responses to ecdysone during Drosophila metamorphosis

Cited by 20 publications

References 30 publications

Australian black field crickets show changes in neural gene expression associated with socially-induced morphological, life-history, and behavioral plasticity

Australian black field crickets show changes in neural gene expression associated with socially-induced morphological, life-history, and behavioral plasticity

TRF2: TRansForming the view of general transcription factors

Drosophila TRF2 is a preferential core promoter regulator

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