2021
DOI: 10.1021/acs.jafc.0c07588 View full text |Buy / Rent full text
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Abstract: Engineering an artificial microbial community for natural product production is a promising strategy. As mono-and dual-culture systems only gave non-detectable or minimal chlorogenic acid (CGA) biosynthesis, here, a polyculture of three recombinant Escherichia coli strains, acting as biosynthetic modules of caffeic acid (CA), quinic acid (QA), and CGA, was designed and used for de novo CGA biosynthesis. An influx transporter of 3-dehydroshikimic acid (DHS)/shikimic acid (SA), ShiA, was introduced into the QA m… Show more

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“…Recently, chlorogenic acid was synthesized using polyculture system (Li et al, 2021) and cell-free biosynthesis system (CFBS) (Niu et al, 2021). The study using the polyculture system divided the whole pathway into three modules (the first module for caffeic acid, the second for quinic acid and the third for chlorogenic acid) and each module was introduced into E. coli.…”
Section: Discussionmentioning
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“…Recently, chlorogenic acid was synthesized using polyculture system (Li et al, 2021) and cell-free biosynthesis system (CFBS) (Niu et al, 2021). The study using the polyculture system divided the whole pathway into three modules (the first module for caffeic acid, the second for quinic acid and the third for chlorogenic acid) and each module was introduced into E. coli.…”
Section: Discussionmentioning
“…To test the applicability of the DS-AS co-culture system in modular co-culture engineering, we selected the biosynthesis of CGA as an example. Caffeic acid (CA) de novo synthesis (CA pathway) and CGA conversion from CA (CGA pathway) are relative independent 12 . We integrated the CA and CGA pathways into the DS-AS co-culture system to form CA and CGA modules with exogeneous quinic acid supplementation, which was expected to reduce the accumulation of toxic intermediate CA and increase the content of the nal product CGA by balancing the proportion between modules (Fig.…”
Section: Modular Co-culture Engineering For Cga Productionmentioning
“…Maintaining the stability of the strain population ratio can be di cult [3][4][5][6][7] . Despite this, many remarkable achievements using modular co-cultivation engineering have been reported [11][12][13][14][15][16][17] . These studies showed the advantages of modularity, but the population ratio did not maintain long-term balance, and each strain grew relatively independently because there was no growth-related relationship among the cocultured strains.…”
Section: Introductionmentioning
“…Alternatively, a polyculture system was designed consisting of three strains with each producing CA, QA, and CGA, respectively. 14 Accordingly, the production of CGA at titers as high as 450, 78, and 88.6 mg/L were reported. Although precursor addition or co-cultivation may be feasible approaches to minimize metabolic interference, the scalable production of CGA would be more feasible and cost-effective through the reconstitution of the whole biosynthetic pathway in a single strain using simple carbon sources (e.g., glucose).…”
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“…Previously, CA or the fermentation broth of another CA-producing strain was supplemented. Alternatively, a polyculture system was designed consisting of three strains with each producing CA, QA, and CGA, respectively . Accordingly, the production of CGA at titers as high as 450, 78, and 88.6 mg/L were reported.…”
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