<i>Cordyceps sinensis</i>
            Cs‐4 restores aging‐associated changes in gene expression and extends lifespan in normal aged mice

Tan, Ningzhi; Barger, Jamie L.; Zhang, Yan; Ferguson, Scott B.; Wu, Zimei; Prolla, Tomas A.; Bartlett, Mark; Zhu, Jia‐Shi

doi:10.1096/fasebj.25.1_supplement.1090.13

Cited by 6 publications

(7 citation statements)

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“…Genotypes #13-14 show alternating reciprocal substitutions of large DNA segments between the genomes of 2 parental fungi, namely, Genotype #1 H. sinensis (Group-A by [66]) and the AB067719-type Group-E fungus (cf. Table 3 and Fig 6), indicating that the biological processes of plasmogamy, karyogamy, chromosomal intertwining interaction, and genetic material recombination occur differentially between the 2 parental fungi regardless of the processes of hereditary variation caused by fungal hybridization or parasexual reproduction [9,48]. The divergent fungal components with altered abundances may participate in the control of the development, maturation and ejection of C. sinensis ascospores.…”

Section: Fungal Factors Involved In the Control Of The Development Ma...mentioning

confidence: 97%

“…Natural Cordyceps sinensis is one of the most highly valued therapeutic agents in traditional Chinese medicine (TCM) and has been used for centuries in clinics as a folk medicine for "Yin-Yang" double invigoration, health maintenance, disease amelioration, and post-disease/ surgery recovery [1,2]. Modern pharmacological studies have validated the therapeutic profile and lifespan-extending properties of natural C. sinensis and its mycelial fermentation products [2][3][4][5][6]. The Chinese Pharmacopeia defines natural C. sinensis as an insect-fungal complex consisting of the fruiting body of Ophiocordyceps sinensis and the remains of Hepialidae moth larva, i.e., natural C. sinensis 6 ¼ O. sinensis fungus [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Differential coexistence of multiple genotypes of Ophiocordyceps sinensis in the stromata, ascocarps and ascospores of natural Cordyceps sinensis

Yao

et al. 2023

PLoS ONE

120

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Objective To examine the differential occurrence of Ophiocordyceps sinensis genotypes in the stroma, stromal fertile portion (SFP) densely covered with numerous ascocarps, and ascospores of natural Cordyceps sinensis. Methods Immature and mature C. sinensis specimens were harvested. Mature C. sinensis specimens were continuously cultivated in our laboratory (altitude 2,200 m). The SFPs (with ascocarps) and ascospores of C. sinensis were collected for microscopic and molecular analyses using species-/genotype-specific primers. Sequences of mutant genotypes of O. sinensis were aligned with that of Genotype #1 Hirsutella sinensis and compared phylogenetically using a Bayesian majority-rule method. Results Fully and semiejected ascospores were collected from the same specimens. The semiejected ascospores tightly adhered to the surface of the asci as observed by the naked eye and under optical and confocal microscopies. The multicellular heterokaryotic ascospores showed uneven staining of nuclei. The immature and mature stromata, SFPs (with ascocarps) and ascospores were found to differentially contain several GC- and AT-biased genotypes of O. sinensis, Samsoniella hepiali, and an AB067719-type fungus. The genotypes within AT-biased Cluster-A in the Bayesian tree occurred in all compartments of C. sinensis, but those within AT-biased Cluster-B were present in immature and mature stromata and SPFs but absent in the ascospores. Genotype #13 of O. sinensis was present in semi-ejected ascospores and Genotype #14 in fully ejected ascospores. GC-biased Genotypes #13–14 featured large DNA segment substitutions and genetic material recombination between the genomes of the parental fungi (H. sinensis and the AB067719-type fungus). These ascosporic offspring genotypes combined with varying abundances of S. hepiali in the 2 types of ascospores participated in the control of the development, maturation and ejection of the ascospores. Conclusion Multiple genotypes of O. sinensis coexist differentially in the stromata, SFPs and 2 types of C. sinensis ascospores, along with S. hepiali and the AB067719-type fungus. The fungal components in different combinations and their dynamic alterations in the compartments of C. sinensis during maturation play symbiotic roles in the lifecycle of natural C. sinensis.

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Section: Fungal Factors Involved In the Control Of The Development Ma...mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Differential coexistence of multiple genotypes of Ophiocordyceps sinensis in the stromata, ascocarps and ascospores of natural Cordyceps sinensis

Yao

et al. 2023

PLoS ONE

120

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“…The therapeutic profiles of Cordyceps sinensis for respiratory, cardiovascular, endocrine, kidney, and liver systems, etc., have been studied using modern pharmacological approaches and summarized [ 2 , 3 ]. The lifespan-extending properties of natural Cordyceps sinensis and its mycelial fermentation products were validated in mice in a 4-year pharmacology study, and the molecular mechanisms have been explored using a whole-gene expression biochip technique [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Altered GC- and AT-biased genotypes of Ophiocordyceps sinensis in the stromal fertile portions and ascospores of natural Cordyceps sinensis

Gao

Yao

et al. 2023

PLoS ONE

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Objective To examine multiple genotypes of Ophiocordyceps sinensis in a semi-quantitative manner in the stromal fertile portion (SFP) densely covered with numerous ascocarps and ascospores of natural Cordyceps sinensis and to outline the dynamic alterations of the coexisting O. sinensis genotypes in different developmental phases. Methods Mature Cordyceps sinensis specimens were harvested and continuously cultivated in our laboratory (altitude 2,254 m). The SFPs (with ascocarps) and fully and semi-ejected ascospores were collected for histological and molecular examinations. Biochip-based single nucleotide polymorphism (SNP) MALDI-TOF mass spectrometry (MS) was used to genotype multiple O. sinensis mutants in the SFPs and ascospores Results Microscopic analysis revealed distinct morphologies of the SFPs (with ascocarps) before and after ascospore ejection and SFP of developmental failure, which, along with the fully and semi-ejected ascospores, were subjected to SNP MS genotyping analysis. Mass spectra showed the coexistence of GC- and AT-biased genotypes of O. sinensis that were genetically and phylogenetically distinct in the SFPs before and after ejection and of developmental failure and in fully and semi-ejected ascospores. The intensity ratios of MS peaks were dynamically altered in the SFPs and the fully and semi-ejected ascospores. Mass spectra also showed transversion mutation alleles of unknown upstream and downstream sequences with altered intensities in the SFPs and ascospores. Genotype #5 of AT-biased Cluster-A maintained a high intensity in all SFPs and ascospores. An MS peak with a high intensity containing AT-biased Genotypes #6 and #15 in pre-ejection SFPs was significantly attenuated after ascospore ejection. The abundance of Genotypes #5‒6 and #16 of AT-biased Cluster-A was differentially altered in the fully and semi-ejected ascospores that were collected from the same Cordyceps sinensis specimens. Conclusion Multiple O. sinensis genotypes coexisted in different combinations with altered abundances in the SFPs prior to and after ejection, the SFP of developmental failure, and the two types of ascospores of Cordyceps sinensis, demonstrating their genomic independence. Metagenomic fungal members present in different combinations and with dynamic alterations play symbiotic roles in different compartments of natural Cordyceps sinensis.

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“…2 However, Cordyceps sinensis supplementation extends longevity in mice by ∼10%. 3 Pro-inflammatory preconditioning is a classic approach to enhance muscle adaptation in humans. 4 Cordyceps has been widely consumed by athletes and sport enthusiasts.…”

Section: Introductionmentioning

confidence: 99%

Cordyceps sinensis accelerates stem cell recruitment to human skeletal muscle after exercise

Dewi,

Liao,

Jean

et al. 2024

Food Funct.

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Cordyceps sinensis Cs‐4 restores aging‐associated changes in gene expression and extends lifespan in normal aged mice

Cited by 6 publications

References 0 publications

Differential coexistence of multiple genotypes of Ophiocordyceps sinensis in the stromata, ascocarps and ascospores of natural Cordyceps sinensis

Differential coexistence of multiple genotypes of Ophiocordyceps sinensis in the stromata, ascocarps and ascospores of natural Cordyceps sinensis

Altered GC- and AT-biased genotypes of Ophiocordyceps sinensis in the stromal fertile portions and ascospores of natural Cordyceps sinensis

Cordyceps sinensis accelerates stem cell recruitment to human skeletal muscle after exercise

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