<i>Chlorella vulgaris</i> supplementation attenuates the progression of liver fibrosis through targeting TGF-β-signaling pathway in the CCl<sub>4</sub>-induced liver fibrosis in rats

Mohseni, Roohollah; Sadeghabadi, Zahra Arab; Karimi, Jamshid; Gholami, Hamid; Ghasemi, Hadi; Ghadimipour, Hamid Reza; Kheiripour, Nejat

doi:10.1080/15569543.2019.1700525

Cited by 7 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The histological investigations of C. vulgaris and/or Orange peels supplementation in irradiated rats showed amelioration in liver fibrosis progression in accordance with the finding obtained by Abdel-Aziz et al 88 It could be attributed to amelioration of oxidative stress status and targeting TGF-β1 signaling pathway. 89…”

Section: Histopathological Observationmentioning

confidence: 99%

Orange peels and Chlorella vulgaris supplementation ameliorate gamma radiation‐induced oxidative stress by regulating TGF‐β and NOX2/NOX4 signaling pathways

Abdelrahman,

Mohammed,

Badawy

et al. 2023

Cell Biochemistry & Function

View full text Add to dashboard Cite

Numerous studies revealed that Chlorella vulgaris and orange peels are potential sources for many valuable compounds such as flavonoids, which are natural polyphenols with antioxidant capacities that lessen oxidative stress via suppressing ROS levels. Thus, this study was designed to investigate their radioprotective efficiency either alone or in combination as natural food supplements. Sixty‐four male Albino rats were divided into eight groups (n = 8) as follows: control, orange peel (10% in diet), C. vulgaris (1% in diet), orange peel + C. vulgaris, gamma irradiated (2Gy twice per week up to 8Gy), orange peel + gamma irradiation, C. vulgaris + gamma irradiation, and orange peel + C. vulgaris + gamma irradiation. After the experiment, blood serums were collected for biochemical analysis, whole bloods were collected for blood picture, bone marrows were collected for GSH, MDA, TGF‐β, NOX2 and NOX4, and liver tissues were collected for histopathological evaluation. Current study revealed that exposure to gamma irradiation induced a significant disturbance in liver function markers (ALT and AST), kidney function markers (urea and creatinine), cholesterol and triglycerides levels in serum. In addition, a significant decrease in WBCs, RBCs, PLT, and Hb in blood of irradiated rats. Moreover, a significant elevation in TGF‐β, NOX2, NOX4 activities, and MDA level, while showed a marked decrease in GSH concentration. Furthermore, hepatic inflammation appeared in the histopathological examination. Orange peels or C. vulgaris treatments showed acceptable amelioration in all measured parameters, combination between orange peels and C. vulgaris showed statistically significant additive amelioration in radiation induced disturbance.

show abstract

Section: Histopathological Observationmentioning

confidence: 99%

Orange peels and Chlorella vulgaris supplementation ameliorate gamma radiation‐induced oxidative stress by regulating TGF‐β and NOX2/NOX4 signaling pathways

Abdelrahman,

Mohammed,

Badawy

et al. 2023

Cell Biochemistry & Function

View full text Add to dashboard Cite

show abstract

“…Okamoto et al demonstrated the beneficial effects of nutrients extracted from Chlorella vulgaris on the primary culture of bovine myoblasts. Furthermore, Mohseni et al found that C. vulgaris extract might impede the progression of liver fibrosis by targeting the TGF-β signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

“…Okamoto et al 12 demonstrated the beneficial effects of nutrients extracted from Chlorella vulgaris on the primary culture of bovine myoblasts. Furthermore, Mohseni et al 13 found that C. vulgaris extract might impede the progression of liver fibrosis by targeting the TGF-β signaling pathway. Mohseni et al 14 also revealed that C. vulgaris extract inhibited liver fibrosis induced by carbon tetrachloride by targeting the Hippo signaling pathway and the AMPK/FOXO1 axis.…”

Section: Introductionmentioning

confidence: 99%

Integrated Omics Approach: Revealing the Mechanism of Auxenochlorella pyrenoidosa Protein Extract Replacing Fetal Bovine Serum for Fish Muscle Cell Culture

Dong,

Jiang,

Chang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

The process of producing cell-cultured meat involves utilizing a significant amount of culture medium, including fetal bovine serum (FBS), which represents a considerable portion of production expense while also raising environmental and safety concerns. This study demonstrated that supplementation with Auxenochlorella pyrenoidosa protein extract (APE) under low-serum conditions substantially increased Carassius auratus muscle (CAM) cell proliferation and heightened the expression of Myf5 compared to the absence of APE. An integrated intracellular metabolomics and proteomics analysis revealed a total of 13 and 67 differentially expressed metabolites and proteins, respectively, after supplementation with APE in the medium containing 5%FBS, modulating specific metabolism and signaling pathways, which explained the application of APE for passage cell culture under lowserum conditions. Further analysis revealed that the bioactive factors in the APE were protein components. Moreover, CAM cells cultured in reconstructed serum-free media containing APE, L-ascorbic acid, insulin, transferrin, selenium, and ethanolamine exhibited significantly accelerated growth in a scale-up culture. These findings suggest a promising alternative to FBS for fish muscle cell culture that can help reduce production costs and environmental impact in the production of cultured meat.

show abstract

“…Nutritionally, CVE is a rich source of fatty acids, amino acids, minerals, vitamins and fibre (Safi et al, 2014). Furthermore, CVE also contains different phytochemical compounds including chlorophyll, tocopherols, ubiquinone and carotenoids (Mohseni et al, 2021; Moradi et al, 2021). Several studies have suggested that CVE , as a dietary supplementation, may attenuate oxidative damage to the DNA, lipids and proteins (Panahi et al, 2016; Sikiru et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Chlorella vulgaris ameliorates testicular toxicity induced by carbon tetrachloride in male rats via modulating oxidative stress

et al. 2022

Self Cite

View full text Add to dashboard Cite

This study was aimed to evaluate the protective effects of Chlorella Vulgaris (CVE) (50 and 100 mg/kg doses) on sperm DNA fragmentation, testis oxidative stress in Carbon tetrachloride (CCL4)-exposed rats. Thirty healthy male Wistar rats were divided into five groups (n = 6): Control; CCl4; CVE; CCl4 + CVE50; CCl4 + CVE100.At the end of the experiment, the testicular oxidative stress parameters were estimated. The Chromomycin A3 (CMA3) and Acridine orange (AO) staining were per-

show abstract

Chlorella vulgaris supplementation attenuates the progression of liver fibrosis through targeting TGF-β-signaling pathway in the CCl₄-induced liver fibrosis in rats

Cited by 7 publications

References 22 publications

Orange peels and Chlorella vulgaris supplementation ameliorate gamma radiation‐induced oxidative stress by regulating TGF‐β and NOX2/NOX4 signaling pathways

Orange peels and Chlorella vulgaris supplementation ameliorate gamma radiation‐induced oxidative stress by regulating TGF‐β and NOX2/NOX4 signaling pathways

Integrated Omics Approach: Revealing the Mechanism of Auxenochlorella pyrenoidosa Protein Extract Replacing Fetal Bovine Serum for Fish Muscle Cell Culture

Chlorella vulgaris ameliorates testicular toxicity induced by carbon tetrachloride in male rats via modulating oxidative stress

Contact Info

Product

Resources

About

Chlorella vulgaris supplementation attenuates the progression of liver fibrosis through targeting TGF-β-signaling pathway in the CCl4-induced liver fibrosis in rats

Cited by 7 publications

References 22 publications

Orange peels and Chlorella vulgaris supplementation ameliorate gamma radiation‐induced oxidative stress by regulating TGF‐β and NOX2/NOX4 signaling pathways

Orange peels and Chlorella vulgaris supplementation ameliorate gamma radiation‐induced oxidative stress by regulating TGF‐β and NOX2/NOX4 signaling pathways

Integrated Omics Approach: Revealing the Mechanism of Auxenochlorella pyrenoidosa Protein Extract Replacing Fetal Bovine Serum for Fish Muscle Cell Culture

Chlorella vulgaris ameliorates testicular toxicity induced by carbon tetrachloride in male rats via modulating oxidative stress

Contact Info

Product

Resources

About

Chlorella vulgaris supplementation attenuates the progression of liver fibrosis through targeting TGF-β-signaling pathway in the CCl₄-induced liver fibrosis in rats