Centella asiaticaalleviates diabetes‐induced changes in fatty acid profile and oxidative damage in rat testis

Abstract

The in vivo effects of Centella asiatica L. Urban (Family: Apiaceae; CA) on diabetesinduced testicular fatty acid misdistribution and oxidative injury were investigated. Diabetic rats were treated with vehicle, CA or metformin daily for 14 days by oral gavage. Fatty acid (FA) content in testis was analysed using gas chromatographyflame ionisation detection while redox indices were measured as peroxide value (PV), malondialdehyde (MDA), oxygen radical antioxidant capacity (ORAC), reduced glutathione (GSH), glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities. Diabetes increased omega-6 (61%), and decreased omega-3 (23%) and monounsaturated fatty acids (MUFA; 18%) compared to non-diabetic controls. Oxidative injury in diabetic rats was confirmed by increases in PV (112%) and MDA (77%) in addition to decreases in GSH (41%) and activities of GST (19%) & GPx (24%) compared to non-diabetic controls. CA treatment led to 17% reduction in omega-6 and 33% rise in MUFA compared to diabetic controls. Additionally, CA ameliorated the oxidative injury and improved antioxidant capacity by increasing GSH (49%), GST (16%) and GPx (23%) when compared to diabetic controls. Data suggest CA potential in alleviating the alterations caused by diabetes in testes through effects on omega-6 and MUFA; and via increased GSH level and dependent enzyme activities.

Ameliorative effects of Anchomanes difformis aqueous extract against oxidative stress in the testes and epididymis of streptozotocin-induced diabetic male Wistar rats

“…The increase in MDA (oxidative stress) in the diabetic testes could have been caused by both the production of ROS that accompanied the established hyperglycemia, and the decrease in antioxidant activity ( Alsenosy et al, 2019 , Sefidgar et al, 2019 ). These findings support the other studies that have reported the association between DM and oxidative stress ( Nna et al, 2019 , Oguntibeju et al, 2020 , Oyenihi et al, 2020 ). Alabi and colleagues (2020) reported the possible significance of A. difformis to extract in ameliorating oxidative stress through the Nrf2 pathway.…”

“…The presence of these compounds might be responsible for the biological activities demonstrated by the plant. As a result, the significant decrease in the reduction of Fe 2+ in the testes of diabetic rats compared to the control groups in this study corroborates with the report of Oyenihi et al (2020) that measured the FRAP capacity of the testes and deduced that antioxidant power was reduced when DM was induced. The antioxidant power may be compromised by oxidative stress ( Erukainure, 2019 , Pieme et al, 2017 ), further confirmed in the present study.…”

“…The increase in blood glucose causes an increase in the production of free radicals through the electron transport chain of the mitochondria, during glucose oxidation ( Nna et al, 2019 ). STZ-induced partial loss of pancreatic beta cells, together with insulin resistance induced by a 10% fructose dosage, has been linked to T2DM symptoms and consequences ( Goboza et al, 2019 , Oyenihi et al, 2020 ). In the present study, blood glucose ≥ 324 mg/dl was indicative of diabetes.…”

“…Previous studies have yielded positive results regarding the effect of some plants for male infertility treatment as a complication caused by oxidative stress ( Asadi et al, 2017 , Oyenihi et al, 2020 ). A. difformis is one of the medicinal plants investigated and found to have positive effects on diabetic parameters, due to its antioxidant content ( Alabi et al, 2020 ).…”

Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-Tetrahydrocannabinol

“…Studies showed that diabetes causes oxidative damage in rat testis. The oxidative damage in diabetic animals was confirmed by elevations in MDA level and diminishment in GSH level (21)(22)(23). Thyagaraju and Muralidhara suggested that PCO levels were higher in diabetic testis and liver tissues than non-diabetics (23).…”