2018
DOI: 10.1002/chem.201705948
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Abstract: Conformational dynamics of RNA molecules play a critical role in governing their biological functions. Measurements of RNA dynamic behavior sheds important light on sites that interact with their binding partners or cellular stimulators. However, such measurements using solution-state NMR are difficult for large RNA molecules (>70 nt; nt=nucleotides) owing to severe spectral overlap, homonuclear C scalar couplings, and line broadening. Herein, a strategic combination of solid-phase synthesis, site-specific iso… Show more

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Cited by 13 publications
(25 citation statements)
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“…This approachw as for example used in refs. [75], [ 85], [ 87] and is valid for exchange rates significantly faster than R 2 and low populations of the excited state. For 2-state processes in the fast-intermediate exchange limit, Luz-Meiboom and Bloom equations [81] can be used to fit the data.…”
Section: Cpmgmentioning
confidence: 99%
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“…This approachw as for example used in refs. [75], [ 85], [ 87] and is valid for exchange rates significantly faster than R 2 and low populations of the excited state. For 2-state processes in the fast-intermediate exchange limit, Luz-Meiboom and Bloom equations [81] can be used to fit the data.…”
Section: Cpmgmentioning
confidence: 99%
“…The exchange rate constants k EX of A75, A88, and A90 were very close to one another within experimental error, whereas the rate parameters of A76 andA 95 were roughly one magnitude higher.T itration with miR-1224 resulted in similar chemicalshift changes for A90 C1' as determined DW for excited states, indicating miR-1224 binding site at A88-A90 bulge. [85] Analysis:B loch-McConnell equations can be used to describe evolution of magnetization in at wo-state intermediate exchange process and model relaxationr ates can be obtained by numerical integration of the matrix and solutions. This procedure has been used for 1 HC PMG data of RNA.…”
Section: Cpmgmentioning
confidence: 99%
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“…The expected micro-to milliseconds timescale of nucleoside dynamics in the active site, and of nucleosides at tertiary interactions should be accessible by using selective 13 C labelling patterns and relaxation dispersion NMR experiments. [306][307][308][309][310] In this way, populations of nucleoside conformations (concerning e.g. ribose pucker, glycosidic bond angles, etc.)…”
Section: Outlook On Further Fundamental Studies On Ribozymes and Deoxmentioning
confidence: 99%