BpGRP1 acts downstream of BpmiR396c/BpGRF3 to confer salt tolerance in Betula platyphylla

Abstract

SummaryGlycine‐rich RNA‐binding proteins (GRPs) have been implicated in the responses of plants to environmental stresses, but the function of GRP genes involved in salt stress and the underlying mechanism remain unclear. In this study, we identified BpGRP1 (glycine‐rich RNA‐binding protein), a Betula platyphylla gene that is induced under salt stress. The physiological and molecular responses to salt tolerance were investigated in both BpGRP1‐overexpressing and suppressed conditions. BpGRF3 (growth‐regulating factor 3) was identified as a regulatory factor upstream of BpGRP1. We demonstrated that overexpression of BpGRF3 significantly increased the salt tolerance of birch, whereas the grf3‐1 mutant exhibited the opposite effect. Further analysis revealed that BpGRF3 and its interaction partner, BpSHMT, function upstream of BpGRP1. We demonstrated that BpmiR396c, as an upstream regulator of BpGRF3, could negatively regulate salt tolerance in birch. Furthermore, we uncovered evidence showing that the BpmiR396c/BpGRF3 regulatory module functions in mediating the salt response by regulating the associated physiological pathways. Our results indicate that BpmiR396c regulates the expression of BpGRF3, which plays a role in salt tolerance by targeting BpGRP1.

How do non‐halophyte locust trees thrive in temperate coastal regions: A study of salinity and multiple environmental factors on water uptake patterns

“…Studies suggest that soil salinization negatively impacts soil microorganisms, soil organisms, and plant metabolism (McDowell et al, 2022;Sahab et al, 2021). Additionally, salinization has adverse effects on soil physical properties, leading to issues such as soil surface crusting, decreased saturated hydraulic conductivity, and indirect impacts on salt accumulation (Liu et al, 2023). These effects, in turn, reduce plant water absorption and evaporation by lowering soil water potential, resulting in significant constraints on water availability.…”

Molecular mechanism of salinity and waterlogging tolerance in mangrove Kandelia obovata

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