<i>Babesia divergens</i>
            in human in Gansu province, China

Wang, Jinming; Zhang, Shangdi; Yang, Jiajing; Liu, Junlong; Zhang, Dekui; Li, Youquan; Luo, Jianxun; Guan, Guiquan; Yin, Hong

doi:10.1080/22221751.2019.1635431

Cited by 27 publications

(33 citation statements)

References 14 publications

(13 reference statements)

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“…have been reported to be responsible for human babesiosis, including B. microti, B. divergens, B. duncani, B. venatorum, B. crass, and Babesia sp. XXB/HangZhou [29,30,31,32,33]. As a causative agent responsible for human babesiosis, the first case caused by B. motasi-like was reported in Korea in 2005 [31].…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Babesia motasi Responsible for Human Babesiosis by Cross-priming Amplification Combined with a Vertical Flow

Wang

Gao

Zhang

et al. 2020

Preprint

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Background Babesia motasi is known as an etiological agent of human and ovine babesiosis. Diagnosis of babesiosis is traditionally performed by microscopy, examining Giemsa-stained thin peripheral blood smears. Rapid detection and accurate identification of species are desirable for clinical care and epidemiological studies. Methods An easy to operate molecular method, which requires less capital equipment and incorporates cross priming amplification combined with a vertical flow (CPA-VF) visualization strip for rapid detection and identification of B. motasi. Results The CPA-VF targets the 18S rRNA gene and has a detection limit of 50 fg per reaction; no cross reaction was observed with other piroplasms infective to sheep or Babesia infective to humans. CPA-VF and real-time (RT)-PCR had sensitivities of 95.2% (95% confidence interval [CI], 78.1-99.4%) and 90.5% (72-97.6%) and specificities of 95.8 (80.5-99.5%) and 97.9 (83.5-99.9%), respectively, versus microscopy and nested (n) PCR combined with gene sequencing. The clinical performance of the CPA-VF assay was evaluated with field blood samples from sheep (n = 240) in Jintai county, Gansu Province, and clinical specimens (n = 492) obtained from patients bitten by ticks. Conclusions Our results indicate that the CPA-VF is a rapid, accurate, nearly instrument-free molecular diagnostic approach for identification of B. motasi. Therefore, it could be an alternative technique for epidemiological investigations and diagnoses of ovine and/or human babesiosis caused by B. motasi, especially in resource-limited regions.

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Section: Discussionmentioning

confidence: 99%

Rapid Detection of Babesia motasi Responsible for Human Babesiosis by Cross-priming Amplification Combined with a Vertical Flow

Wang

Gao

Zhang

et al. 2020

Preprint

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“…Predominately, three Babesia spp., Babesia microti, B. divergens and B. duncani, have been described to be involved in human infections in the USA, Europe and Asia [4,5]. Recently, two newly emerging Babesia species, named as B. motasi and B. crassa, which were previously reported as causative agents of ovine babesiaosis, have been sporadically reported in cases of human babesiosis in Asia [6][7][8][9][10]. As a causative agent responsible for human babesiosis, the first case caused by B. motasi-like was reported in Korea in 2005 [6].…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Babesia motasi responsible for human babesiosis by cross-priming amplification combined with a vertical flow

et al. 2020

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View full text Add to dashboard Cite

Background: Babesia motasi is known as an etiological agent of human and ovine babesiosis. Diagnosis of babesiosis is traditionally performed by microscopy, examining Giemsa-stained thin peripheral blood smears. Rapid detection and accurate identification of species are desirable for clinical care and epidemiological studies. Methods: An easy to operate molecular method, which requires less capital equipment and incorporates crosspriming amplification combined with a vertical flow (CPA-VF) visualization strip for rapid detection and identification of B. motasi. Results: The CPA-VF targets the 18S rRNA gene and has a detection limit of 50 fg per reaction; no cross reaction was observed with other piroplasms infective to sheep or Babesia infective to humans. CPA-VF and real-time (RT)-PCR had sensitivities of 95.2% (95% confidence interval, CI 78.1-99.4%) and 90.5% (95% CI 72-97.6%) and specificities of 95.8 (95% CI 80.5-99.5%) and 97.9 (95% CI 83.5-99.9%), respectively, versus microscopy and nested (n) PCR combined with gene sequencing. The clinical performance of the CPA-VF assay was evaluated with field blood samples from sheep (n = 340) in Jintai county, Gansu Province, and clinical specimens (n = 492) obtained from patients bitten by ticks. Conclusions: Our results indicate that the CPA-VF is a rapid, accurate, nearly instrument-free molecular diagnostic approach for identification of B. motasi. Therefore, it could be an alternative technique for epidemiological investigations and diagnoses of ovine and/or human babesiosis caused by B. motasi, especially in resource-limited regions.

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“…Predominately, three Babesia spp., Babesia microti, B. divergens, and B. duncani, have been described to be involved in human infections in the United States, Europe, and Asia [4,5]. Recently, two newly emerging Babesia species, named as B. motasi and B. crassa, which were previously reported as causative agents of ovine babesiaosis, have been sporadically reported in cases of human babesiosis in Asia [6,7,8,9,10]. As a causative agent responsible for human babesiosis, the rst case caused by B. motasi-like was reported in Korea in 2005 [11].…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Babesia motasi Responsible for Human Babesiosis by Cross-priming Amplification Combined with a Vertical Flow

Wang

Gao

Zhang

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Babesia motasi is known as an etiological agent of human and ovine babesiosis. Diagnosis of babesiosis is traditionally performed by microscopy, examining Giemsa-stained thin peripheral blood smears. Rapid detection and accurate identification of species are desirable for clinical care and epidemiological studies. Methods: An easy to operate molecular method, which requires less capital equipment and incorporates cross priming amplification combined with a vertical flow (CPA-VF) visualization strip for rapid detection and identification of B. motasi. Results: The CPA-VF targets the 18S rRNA gene and has a detection limit of 50 fg per reaction; no cross reaction was observed with other piroplasms infective to sheep or Babesia infective to humans. CPA-VF and real-time (RT)-PCR had sensitivities of 95.2% (95% confidence interval [CI], 78.1-99.4%) and 90.5% (72-97.6%) and specificities of 95.8 (80.5-99.5%) and 97.9 (83.5-99.9%), respectively, versus microscopy and nested (n) PCR combined with gene sequencing. The clinical performance of the CPA-VF assay was evaluated with field blood samples from sheep (n = 340) in Jintai county, Gansu Province, and clinical specimens (n = 492) obtained from patients bitten by ticks. Conclusions: Our results indicate that the CPA-VF is a rapid, accurate, nearly instrument-free molecular diagnostic approach for identification of B. motasi. Therefore, it could be an alternative technique for epidemiological investigations and diagnoses of ovine and/or human babesiosis caused by B. motasi, especially in resource-limited regions.

show abstract

Babesia divergens in human in Gansu province, China

Cited by 27 publications

References 14 publications

Rapid Detection of Babesia motasi Responsible for Human Babesiosis by Cross-priming Amplification Combined with a Vertical Flow

Rapid Detection of Babesia motasi Responsible for Human Babesiosis by Cross-priming Amplification Combined with a Vertical Flow

Rapid detection of Babesia motasi responsible for human babesiosis by cross-priming amplification combined with a vertical flow

Rapid Detection of Babesia motasi Responsible for Human Babesiosis by Cross-priming Amplification Combined with a Vertical Flow

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