<i>Arabidopsis thaliana BOR4</i>is upregulated under high boron conditions and confers tolerance to high boron

Miwa, Kyoko; Aibara, Izumi; Fujiwara, Toru

doi:10.1080/00380768.2013.866524

Cited by 49 publications

(36 citation statements)

References 23 publications

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“…Thus, tolerant barley cultivars (‘Sahara’) to B toxicity are characterized by a lower leaf B concentration than sensitive cultivars (‘Clipper’) (Roessner et al , Sutton et al ). In Arabidopsis roots, BOR4 gene, which encodes a B‐efflux transporter, plays a crucial role in the mechanism of B exclusion under B toxicity conditions (Miwa et al , , Takano et al ). In agreement with the higher transpiration rate of the nced3‐2 mutant (Fig.…”

Section: Discussionmentioning

confidence: 99%

Abscisic acid and transpiration rate are involved in the response to boron toxicity in Arabidopsis plants

Macho-Rivero

Camacho-Cristóbal

Herrera-Rodríguez

et al. 2017

Physiologia Plantarum

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Boron (B) is an essential microelement for vascular plant development, but its toxicity is a major problem affecting crop yields in arid and semi-arid areas of the world. In the literature, several genes involved in abscisic acid (ABA) signalling and responses are upregulated in Arabidopsis roots after treatment with excess B. It is known that the AtNCED3 gene, which encodes a crucial enzyme for ABA biosynthesis, plays a key role in the plant response to drought stress. In this study, root AtNCED3 expression and shoot ABA content were rapidly increased in wild-type plants upon B-toxicity treatment. The Arabidopsis ABA-deficient nced3-2 mutant had higher transpiration rate, stomatal conductance and accumulated more B in their shoots than wild-type plants, facts that were associated with the lower levels of ABA in this mutant. However, in wild-type plants, B toxicity caused a significant reduction in stomatal conductance, resulting in a decreased transpiration rate. This response could be a mechanism to limit the transport of excess B from the roots to the leaves under B toxicity. In agreement with the higher transpiration rate of the nced3-2 mutant, this genotype showed an increased leaf B concentration and damage upon exposure to 5 mM B. Under B toxicity, ABA application decreased B accumulation in wild-type and nced3-2 plants. In summary, this work shows that excess B applied to the roots leads to rapid changes in AtNCED3 expression and gas exchange parameters that would contribute to restrain the B entry into the leaves, this effect being mediated by ABA.

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Section: Discussionmentioning

confidence: 99%

Abscisic acid and transpiration rate are involved in the response to boron toxicity in Arabidopsis plants

Macho-Rivero

Camacho-Cristóbal

Herrera-Rodríguez

et al. 2017

Physiologia Plantarum

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“…Reduced expression lowers the passive in ux of B from the root surface into the root xylem when the concentration of B in soil is high. Also, increased expression of boron e ux transporter gene BOR4 removes excess B from their roots, reducing B transport to the foliage [20,21,22,23,24,25]. A similar mechanism has been demonstrated in B. rapa [26,27].…”

Section: Introductionmentioning

confidence: 57%

Boron Sensitivity and Storage in Brassica Napus and Its Potential for Clubroot Management

Gossen¹,

Wang

McLean

et al. 2020

Preprint

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Clubroot [Plasmodiophora brassica] severity in canola (Brassica napus) can be suppressed by addition of boron (B), but excess B produces phytotoxicity. However, some lines of a closely related species, B. rapa, are relatively insensitive to high levels of B. Assessment of 150 accessions of B. napus and B. rapa treated with 0, 8 and 16 kg B ha-1 as disodium octaborate tetrahydrate (trade name Solubor) identified several lines that were relatively insensitive to high levels of B. In 2015, 88 lines were planted in a clubroot-infested plot in Ontario, Canada in a replicated split-plot design, where the subplot treatments were 8 kg ha-1 of B versus a non-treated control. Boron-insensitive lines generally had slightly lower clubroot severity than sensitive lines, even in the control with no added B. Application of B reduced clubroot slightly in the most sensitive lines, but produced a large and significant reduction in severity in the most insensitive lines. Assessment of five insensitive lines under controlled conditions supported the observation that application of B substantially reduced clubroot severity in insensitive lines. Comparison of two sensitive and two insensitive lines each of B. napus and B. rapa using Boron K-edge X-ray absorption near-edge structure (XANES) spectra from a synchrotron-based soft-X-ray beamline showed that some insensitive lines were able to extract B efficiently from a soil that contained very low levels of B. The spectra of sensitive and insensitive lines differed when additional B was applied, which demonstrated that the insensitive lines stored B (and other elements such as S and P) differently than the sensitive lines. Differences in the amount and type of storage likely affect the susceptibility to clubroot, and merits further study.

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“…Kaur et al. () discussed the aim to find a candidate B transporter gene like those isolated in Arabidopsis (Miwa, Aibara, & Fujiwara, ; Miwa & Fujiwara, ). Studies in Arabidopsis , wheat, rice and M. truncatula have revealed genes for efflux‐type B transporters and major intrinsic protein (MIP) family controlling B toxicity tolerance (Bogacki et al., ; Takano et al., , ).…”

Section: Discussionmentioning

confidence: 99%

“…The whole genome sequencing data available were also used to assist in the identification of potential candidate genes at this locus, which would enable the development of a diagnostic marker. Kaur et al (2014) discussed the aim to find a candidate B transporter gene like those isolated in Arabidopsis (Miwa, Aibara, & Fujiwara, 2014;Miwa & Fujiwara, 2011). Studies in Arabidopsis, wheat, rice and M. truncatula have revealed genes for efflux-type B transporters and major intrinsic protein (MIP) family controlling B toxicity tolerance (Bogacki et al, 2013;Takano et al, 2002Takano et al, , 2006.…”

Section: Genetic Mapping Qtl Analysis and Marker Validationmentioning

confidence: 99%

Breeding for boron tolerance in lentil (Lens culinaris Medik.) using a high‐throughput phenotypic assay and molecular markers

et al. 2018

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This study describes the identification of a quantitative trait locus (QTL) in the recombinant inbred line population of ILL2024 × ILL6788 and subsequent validation of associated molecular markers. A high‐quality genetic linkage map was constructed with 758 markers that cover 1,057 cM, with an average intermarker distance of 2 cM. QTL analysis revealed a single genomic region on Lc2 to be associated with B tolerance and accounted for up to 76% of phenotypic variation (Vp). The best markers for B tolerance were assessed for their utility in routine breeding applications using validation panels of diverse lentil germplasm and breeding material derived from ILL2024. A marker generated from the dense genetic map of this study was found to be the most accurate of all markers available for B tolerance in lentil, with a success rate of 93% within a large breeding pool derived from ILL2024. However, given the number of the unrelated lines for which the marker–trait association was not conserved, B tolerance screening is still required at later stages to confirm predicted phenotypes.

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Arabidopsis thaliana BOR4is upregulated under high boron conditions and confers tolerance to high boron

Cited by 49 publications

References 23 publications

Abscisic acid and transpiration rate are involved in the response to boron toxicity in Arabidopsis plants

Abscisic acid and transpiration rate are involved in the response to boron toxicity in Arabidopsis plants

Boron Sensitivity and Storage in Brassica Napus and Its Potential for Clubroot Management

Breeding for boron tolerance in lentil (Lens culinaris Medik.) using a high‐throughput phenotypic assay and molecular markers

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