<i>Arabidopsis</i> Chloroplast RNA Binding Proteins CP31A and CP29A Associate with Large Transcript Pools and Confer Cold Stress Tolerance by Influencing Multiple Chloroplast RNA Processing Steps

Kupsch, Christiane; Ruwe, Hannes; Gusewski, Sandra; Tillich, Michael; Small, Ian; Schmitz‐Linneweber, Christian

doi:10.1105/tpc.112.103002

Cited by 111 publications

(182 citation statements)

References 58 publications

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“…If we had chosen bulk sequencing to assay the editing extent in the orrm1 mutant plant, there would not have seen any detectable edited peak for the 12 sites whose editing extent in the orrm1 mutant is < 0.1 (Table S1). Recently, Kupsch et al (44) found that CP31A associates with large transcript pools and confers cold stress tolerance by influencing multiple chloroplast RNA processing events (44). The authors indicate that relative to its effect on RNA stability, the effect of CP31A on editing extent is minor.…”

Section: Discussionmentioning

confidence: 99%

An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize

Sun

Germain

Giloteaux

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

133

185

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Plant RNA editing modifies cytidines (C) to uridines (U) at specific sites in the transcripts of both mitochondria and plastids. Specific targeting of particular Cs is achieved by pentatricopeptide proteins that recognize cis elements upstream of the C that is edited. Members of the RNA-editing factor interacting protein (RIP) family in Arabidopsis have recently been shown to be essential components of the plant editosome. We have identified a gene that contains a pair of truncated RIP domains (RIP-RIP). Unlike any previously described RIP family member, the encoded protein carries an RNA recognition motif (RRM) at its C terminus and has therefore been named Organelle RRM protein 1 (ORRM1). ORRM1 is an essential plastid editing factor; in Arabidopsis and maize mutants, RNA editing is impaired at particular sites, with an almost complete loss of editing for 12 sites in Arabidopsis and 9 sites in maize. Transfection of Arabidopsis orrm1 mutant protoplasts with constructs encoding a region encompassing the RIP-RIP domain or a region spanning the RRM domain of ORRM1 demonstrated that the RRM domain is sufficient for the editing function of ORRM1 in vitro. According to a yeast two-hybrid assay, ORRM1 interacts selectively with pentatricopeptide transfactors via its RIP-RIP domain. Phylogenetic analysis reveals that the RRM in ORRM1 clusters with a clade of RRM proteins that are targeted to organelles. Taken together, these results suggest that other members of the ORRM family may likewise function in RNA editing.

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Section: Discussionmentioning

confidence: 99%

An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize

Sun

Germain

Giloteaux

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

133

185

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“…One includes the proteins that bind a large set of chloroplast RNAs with little gene specificity, such as the cpRNP proteins that intervene at multiples steps of gene expression (Kupsch et al, 2012). The other consists of the proteins that bind to a restricted subset of plastid transcripts or to a single one and have more specific roles.…”

Section: Mac1 Is Phosphorylated To Different Extents Depending On Gromentioning

confidence: 99%

A Nucleus-Encoded Chloroplast Phosphoprotein Governs Expression of the Photosystem I Subunit PsaC in Chlamydomonas reinhardtii

Douchi

Longoni

et al. 2016

Plant Cell

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The nucleo-cytoplasmic compartment exerts anterograde control on chloroplast gene expression through numerous proteins that intervene at posttranscriptional steps. Here, we show that the maturation of psaC mutant (mac1) of Chlamydomonas reinhardtii is defective in photosystem I and fails to accumulate psaC mRNA. The MAC1 locus encodes a member of the Half-A-Tetratricopeptide (HAT) family of super-helical repeat proteins, some of which are involved in RNA transactions. The Mac1 protein localizes to the chloroplast in the soluble fraction. MAC1 acts through the 59 untranslated region of psaC transcripts and is required for their stability. Small RNAs that map to the 59end of psaC RNA in the wild type but not in the mac1 mutant are inferred to represent footprints of MAC1-dependent protein binding, and Mac1 expressed in bacteria binds RNA in vitro. A coordinate response to iron deficiency, which leads to dismantling of the photosynthetic electron transfer chain and in particular of photosystem I, also causes a decrease of Mac1. Overexpression of Mac1 leads to a parallel increase in psaC mRNA but not in PsaC protein, suggesting that Mac1 may be limiting for psaC mRNA accumulation but that other processes regulate protein accumulation. Furthermore, Mac 1 is differentially phosphorylated in response to iron availability and to conditions that alter the redox balance of the electron transfer chain.

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“…Chloroplasts from 5-week-old Arabidopsis plants were prepared as described by Kupsch et al (2012). Stroma was separated from membranes by centrifugation at 40,000g for 10 min.…”

Section: Protein Isolation Western Blotting and Rip-chip Analysesmentioning

confidence: 99%

“…Stroma was separated from membranes by centrifugation at 40,000g for 10 min. Membranes were washed twice, and equal volumes of total chloroplasts, stroma, and membranes were loaded onto an SDS-PAGE system and investigated by western blot as described (Kupsch et al, 2012).…”

Section: Protein Isolation Western Blotting and Rip-chip Analysesmentioning

confidence: 99%

A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNA^Ile(GAU)

Romani

Manavski²,

Morosetti

et al. 2015

Plant Physiol.

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Plastid gene expression is crucial for organelle function, but the factors that control it are still largely unclear. Members of the socalled mitochondrial transcription termination factor (mTERF) family are found in metazoans and plants and regulate organellar gene expression at different levels. Arabidopsis (Arabidopsis thaliana) mTERF6 is localized in chloroplasts and mitochondria, and its knockout perturbs plastid development and results in seedling lethality. In the leaky mterf6-1 mutant, a defect in photosynthesis is associated with reduced levels of photosystem subunits, although corresponding messenger RNA levels are unaffected, whereas translational capacity and maturation of chloroplast ribosomal RNAs (rRNAs) are perturbed in mterf6-1 mutants. Bacterial one-hybrid screening, electrophoretic mobility shift assays, and coimmunoprecipitation experiments reveal a specific interaction between mTERF6 and an RNA sequence in the chloroplast isoleucine transfer RNA gene (trnI.2) located in the rRNA operon. In vitro, recombinant mTERF6 bound to its plastid DNA target site can terminate transcription. At present, it is unclear whether disturbed rRNA maturation is a primary or secondary defect. However, it is clear that mTERF6 is required for the maturation of trnI.2. This points to an additional function of mTERFs.

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Arabidopsis Chloroplast RNA Binding Proteins CP31A and CP29A Associate with Large Transcript Pools and Confer Cold Stress Tolerance by Influencing Multiple Chloroplast RNA Processing Steps

Cited by 111 publications

References 58 publications

An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize

An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize

A Nucleus-Encoded Chloroplast Phosphoprotein Governs Expression of the Photosystem I Subunit PsaC in Chlamydomonas reinhardtii

A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNA^Ile(GAU)

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Arabidopsis Chloroplast RNA Binding Proteins CP31A and CP29A Associate with Large Transcript Pools and Confer Cold Stress Tolerance by Influencing Multiple Chloroplast RNA Processing Steps

Cited by 111 publications

References 58 publications

An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize

An RNA recognition motif-containing protein is required for plastid RNA editing in Arabidopsis and maize

A Nucleus-Encoded Chloroplast Phosphoprotein Governs Expression of the Photosystem I Subunit PsaC in Chlamydomonas reinhardtii

A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNAIle(GAU)

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A Member of the Arabidopsis Mitochondrial Transcription Termination Factor Family Is Required for Maturation of Chloroplast Transfer RNA^Ile(GAU)