<i>ANT1</i>, an Aromatic and Neutral Amino Acid Transporter in Arabidopsis

Chen, Lishan; Ortíz‐López, Adriana; Jung, Alan P.; Bush, Daniel R.

doi:10.1104/pp.125.4.1813

Cited by 114 publications

(91 citation statements)

References 32 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BLAST analysis revealed that the rice Bh4 encoded an amino acid transporter. Phylogenetic comparison of BH4 protein and other amino acid transporters showed that BH4 was closely related to AtANT1, an aromatic and neutral amino acid transporter in Arabidopsis (Arabidopsis thaliana; Chen et al, 2001; Fig. 4A; Supplemental Fig.…”

Section: Bh4 Encodes An Amino Acid Transporter Protein and Is Specifimentioning

confidence: 99%

Genetic Control of a Transition from Black to Straw-White Seed Hull in Rice Domestication

et al. 2011

View full text Add to dashboard Cite

The genetic mechanism involved in a transition from the black-colored seed hull of the ancestral wild rice (Oryza rufipogon and Oryza nivara) to the straw-white seed hull of cultivated rice (Oryza sativa) during grain ripening remains unknown. We report that the black hull of O. rufipogon was controlled by the Black hull4 (Bh4) gene, which was fine-mapped to an 8.8-kb region on rice chromosome 4 using a cross between O. rufipogon W1943 (black hull) and O. sativa indica cv Guangluai 4 (straw-white hull). Bh4 encodes an amino acid transporter. A 22-bp deletion within exon 3 of the bh4 variant disrupted the Bh4 function, leading to the straw-white hull in cultivated rice. Transgenic study indicated that Bh4 could restore the black pigment on hulls in cv Guangluai 4 and Kasalath. Bh4 sequence alignment of all taxa with the outgroup Oryza barthii showed that the wild rice maintained comparable levels of nucleotide diversity that were about 70 times higher than those in the cultivated rice. The results from the maximum likelihood Hudson-Kreitman-Aguade test suggested that the significant reduction in nucleotide diversity in rice cultivars could be caused by artificial selection. We propose that the straw-white hull was selected as an important visual phenotype of nonshattered grains during rice domestication.

show abstract

Section: Bh4 Encodes An Amino Acid Transporter Protein and Is Specifimentioning

confidence: 99%

Genetic Control of a Transition from Black to Straw-White Seed Hull in Rice Domestication

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Phylogenetic relationship between the AtProTs and related proteins. The analysis was performed using the aligned protein sequences of the Arabidopsis ATF gene family that, in addition to the ProTs, contains several groups of amino acid permeases (AAPs, LHTs, ANT-like proteins) as well as potential auxin transporters (AUX1; Fischer et al, 1998;Chen et al, 2001;Wipf et al, 2002). Proteins from other plant species were included for the ProT-like and the At1g08230-like proteins.…”

Section: Atprot3 Is a Novel Member Of The Aprot Gene Familymentioning

confidence: 99%

The AtProT Family. Compatible Solute Transporters with Similar Substrate Specificity But Differential Expression Patterns

Grallath

Weimar²,

Meyer³

et al. 2005

Plant Physiology

159

149

View full text Add to dashboard Cite

Proline transporters (ProTs) mediate transport of the compatible solutes Pro, glycine betaine, and the stress-induced compound g-aminobutyric acid. A new member of this gene family, AtProT3, was isolated from Arabidopsis (Arabidopsis thaliana), and its properties were compared to AtProT1 and AtProT2. Transient expression of fusions of AtProT and the green fluorescent protein in tobacco (Nicotiana tabacum) protoplasts revealed that all three AtProTs were localized at the plasma membrane. Expression in a yeast (Saccharomyces cerevisiae) mutant demonstrated that the affinity of all three AtProTs was highest for glycine betaine (K m 5 0.1-0.3 mM), lower for Pro (K m 5 0.4-1 mM), and lowest for g-aminobutyric acid (K m 5 4-5 mM). Relative quantification of the mRNA level using real-time PCR and analyses of transgenic plants expressing the b-glucuronidase (uidA) gene under control of individual AtProT promoters showed that the expression pattern of AtProTs are complementary. AtProT1 expression was found in the phloem or phloem parenchyma cells throughout the whole plant, indicative of a role in long-distance transport of compatible solutes. b-Glucuronidase activity under the control of the AtProT2 promoter was restricted to the epidermis and the cortex cells in roots, whereas in leaves, staining could be demonstrated only after wounding. In contrast, AtProT3 expression was restricted to the above-ground parts of the plant and could be localized to the epidermal cells in leaves. These results showed that, although intracellular localization, substrate specificity, and affinity are very similar, the transporters fulfill different roles in planta.

show abstract

“…The ATF1 superfamily (amino acid transporter family 1) consists of several subfamilies: the AAPs (amino acid permeases), the LHTs (lysine histidine transporters), the ProTs (proline and compatible solutes transporters), the AUXs (putative auxin transporter) and several new subfamilies (25). One transporter, ANT1, of this new subfamily has been characterized recently as a transporter for neutral and aromatic amino acids (26). The CAT subfamily (cationic amino acid transporters) is a member of the second plant amino acid transporter family, namely the APC-superfamily (24,27).…”

mentioning

confidence: 99%

High Affinity Amino Acid Transporters Specifically Expressed in Xylem Parenchyma and Developing Seeds of Arabidopsis

Okumoto¹,

Schmidt²,

Tegeder³

et al. 2002

Journal of Biological Chemistry

176

167

View full text Add to dashboard Cite

ANT1, an Aromatic and Neutral Amino Acid Transporter in Arabidopsis

Cited by 114 publications

References 32 publications

Genetic Control of a Transition from Black to Straw-White Seed Hull in Rice Domestication

Genetic Control of a Transition from Black to Straw-White Seed Hull in Rice Domestication

The AtProT Family. Compatible Solute Transporters with Similar Substrate Specificity But Differential Expression Patterns

High Affinity Amino Acid Transporters Specifically Expressed in Xylem Parenchyma and Developing Seeds of Arabidopsis

Contact Info

Product

Resources

About